trxA:Gene: Difference between revisions

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!align=left |Standard name
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''trxA''  
''trxA''
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!align=left |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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Thioredoxin
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!align=left |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK3773, b3781, JW5856, fipA, tsnC, dasC''<ref name='LIB:Riley_2006'/>
''ECK3773, b3781, JW5856, fipA, tsnC, dasC''<ref name='LIB:Riley_2006'/>
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Revision as of 21:50, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

trxA

Mnemonic

Thioredoxin

Synonyms

ECK3773, b3781, JW5856, fipA, tsnC, dasC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.43 minutes, 85.43 minutes 

MG1655: 3963784..3964113
gbrowse_img.php?coord=3963784..3964113&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3927561..3927890
gbrowse_img.php?coord=3927561..3927890&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3670920..3670591
gbrowse_img.php?coord=3670591..3670920&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4062704..4063033
gbrowse_img.php?coord=4062704..4063033&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtrxA (Keio:JW5856)

deletion

deletion

PMID:16738554[2]

Shigen

trxA1(Am)

PMID:337124[3] PMID:3881756[4]

CGSC:5031

amber (UAG) mutation

ΔtrxA307

CGSC:10957

trxA11(ts)

temperature sensitive

PMID:3881756[4]

CGSC:88597


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5856

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGCGATAAAATTATTCACCT

Primer 2:CCtGCCAGGTTAGCGTCGAGGAA

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[6]

est. P1 cotransduction: 69% [7]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[6]

est. P1 cotransduction: 14% [7]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11031

EchoBASE (EcoliWiki Page)

EchoBASE:EB1024

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3781

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4701

EcoGene (EcoliWiki Page)

EcoGene:EG11031

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TRXA

CGSC (EcoliWiki Page)

CGSC:65

ASAP (EcoliWikiPage)

ASAP:ABE-0012357


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Mark, DF et al. (1977) Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol. Gen. Genet. 155 145-52 PubMed
  4. ↑ 4.0 4.1 Russel, M & Model, P (1985) Thioredoxin is required for filamentous phage assembly. Proc. Natl. Acad. Sci. U.S.A. 82 29-33 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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