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{{Tab|Quickview|yffM}}{{Tab|Gene|yffM}}{{Tab|Gene Product(s)|yffM}}{{Tab|Expression|yffM}}{{Tab|Evolution|yffM}}{{Tab On|On One Page|yffM}}{{TabsBottom}}
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Latest revision as of 05:20, 29 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yffM

Gene Synonym(s)

ECK2439, b2444, unique to MG1655[1]

Product Desc.

CPZ-55 prophage; predicted protein[2][3]

Function unknown, Eut/CPZ-55 prophage[4]

Product Synonyms(s)

CPZ-55 prophage; predicted protein[1], B2444[2][1], YffM[2][1] , ECK2439, b2444

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yffM[2]

Regulation/Activity
Quick Links



        

See Help:Quickview for help with entering information in the Quickview table.

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yffM

Mnemonic

Systematic nomenclature

Synonyms

ECK2439, b2444, unique to MG1655[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

55.16 minutes 

MG1655: 2559390..2559635
<gbrowseImage> name=NC_000913:2559390..2559635 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2445195..2445440
<gbrowseImage> name=NC_012759:2445195..2445440 source=BW2952 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2651155..2651400
<gbrowseImage> name=NC_010473:2651155..2651400 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[5]

est. P1 cotransduction: 10% [6]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 25% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7274

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14174

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003848

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946921

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3926

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008061

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YffM

Synonyms

CPZ-55 prophage; predicted protein[1], B2444[2][1], YffM[2][1] , ECK2439, b2444

Product description

CPZ-55 prophage; predicted protein[2][3]

Function unknown, Eut/CPZ-55 prophage[4]

EC number (for enzymes)



Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes


Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MMVIRHECPS YCIAQKRVAL REFSELVLGT LSLLLEQKTN GKCSASLYDC SEEEKLFVKR
LKLIKADIHA QLKACDCDIS E
Length

81

Mol. Wt

9.205 kDa

pI

7.2 (calculated)

Extinction coefficient

2,980 - 3,730 (calc based on 2 Y, 0 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yffM taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130369

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946921

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008061

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P76544

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7274

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14174

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946921

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003848

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3926

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yffM

Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2559370..2559410 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2444 (EcoliWiki Page)

NCBI GEO profiles for yffM

microarray

GenExpDB:b2444 (EcoliWiki Page)

Summary of data for yffM from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

<protect>

Notes

Accessions Related to yffM Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7274

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3926

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2444

EcoGene

EcoGene:EG14174

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003848

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008061

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments



Do-It-Yourself Web Tools


Notes

Families

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Database Accession Notes

PFAM (EcoliWiki Page)

PFAM:PF00589

edit table


Notes

Families

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See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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