iscS:Gene: Difference between revisions
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Primer 2:CCATGATGAGCCCATTCGATGCT | Primer 2:CCATGATGAGCCCATTCGATGCT | ||
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[[:Category:Kohara_Phage:5E10|5E10]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=430 Genobase] | |||
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PMID:3038334 | |||
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est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/> <br /> | est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zff-208::Tn10 | Synonyms:zff-208::Tn10 | ||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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Revision as of 20:31, 21 September 2011
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
iscS |
|---|---|
| Mnemonic |
Iron-sulfur cluster |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
57.3 minutes |
MG1655: 2659553..2658339 |
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NC_012967: 2582230..2581016 |
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NC_012759: 2544144..2545358 |
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W3110 |
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W3110: 2660187..2658973 |
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DH10B: 2751318..2750104 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2658339 |
Edman degradation |
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| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔiscS (Keio:JW2514) |
deletion |
deletion |
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iscSC328A |
C328A |
Loss of cysteine desulfurization |
seeded from UniProt:P0A6B7 | ||||
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ΔiscS776::kan |
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| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2514 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAATTACCGATTTATCTCGA Primer 2:CCATGATGAGCCCATTCGATGCT | |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 3% [8] | ||
|
Linked marker |
est. P1 cotransduction: 85% [8] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).