pykF:Gene: Difference between revisions
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Primer 2:CCaAGGACGTGAACAGATGCGGT | Primer 2:CCaAGGACGTGAACAGATGCGGT | ||
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[[:Category:Kohara_Phage:13H4|13H4]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=319 Genobase] | |||
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PMID:3038334 | |||
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est. P1 cotransduction: 42% <ref name='LIB:Nichols_linked_Tn'/> <br /> | est. P1 cotransduction: 42% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zdh-925::Tn10 | Synonyms:zdh-925::Tn10 | ||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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Revision as of 21:12, 21 September 2011
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
pykF |
|---|---|
| Mnemonic |
Pyruvate kinase |
| Synonyms |
ECK1672, b1676, JW1666[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
37.8 minutes |
MG1655: 1753722..1755134 |
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NC_012967: 1732965..1734377 |
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NC_012759: 1645781..1647193 |
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|
W3110 |
|
W3110: 1757412..1758824 |
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DH10B: 1844293..1845705 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1753722 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpykF (Keio:JW1666) |
deletion |
deletion |
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|
pykF::Tn5KAN-2 (FB20420) |
Insertion at nt 400 in Plus orientation |
does not contain pKD46 | |||||
|
pykF::Tn5KAN-2 (FB20421) |
Insertion at nt 400 in Plus orientation |
contains pKD46 | |||||
|
ΔpykF751::kan |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1666 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAAGACCAAAATTGTTTG Primer 2:CCaAGGACGTGAACAGATGCGGT | |
|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: % [9] | ||
|
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: 42% [9] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Valentini, G et al. (1991) Bacterial pyruvate kinases have a shorter N-terminal domain. Biol. Chem. Hoppe-Seyler 372 91-3 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).