pykF:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PykF

Synonyms

pyruvate kinase I[1], B1676[2][1], PykF[2][1] , ECK1672, JW1666, b1676

Product description

pyruvate kinase I[2][3];

Component of pyruvate kinase I[2][3]

Pyruvate kinase I, fructose 1,6-bisphosphate-activated[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001697

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015793

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015794

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015813

F

Seeded from EcoCyc (v14.0)

complete

GO:0004743

pyruvate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001697

F

Seeded from EcoCyc (v14.0)

complete

GO:0004743

pyruvate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015793

F

Seeded from EcoCyc (v14.0)

complete

GO:0004743

pyruvate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015794

F

Seeded from EcoCyc (v14.0)

complete

GO:0004743

pyruvate kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.40

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001697

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015793

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015794

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0324

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001697

F

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015793

F

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015794

F

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0630

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of pyruvate kinase I

could be indirect

Protein

yraI

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

rbsR

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

rpsA

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

infB

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

deoD

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

tyrS

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

cyaA

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

dnaK

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

sxy

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

prs

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

pflB

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

pldA

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

yjcC

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

hybO

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

yheS

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

imp

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

gfcD

PMID:16606699[6]

Experiment(s):EBI-1140456

Protein

rpsS

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

murF

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplQ

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

ybgI

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplI

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):28.698600

Protein

rpsM

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

tig

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):25.306932

Protein

rplM

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplB

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):12.914124

Protein

nrdD

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):6.517026

Protein

yibL

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

hupB

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplY

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rluB

PMID:19402753[7]

MALDI(Z-score):18.719238

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKKTKIVCTI GPKTESEEML AKMLDAGMNV MRLNFSHGDY AEHGQRIQNL RNVMSKTGKT
AAILLDTKGP EIRTMKLEGG NDVSLKAGQT FTFTTDKSVI GNSEMVAVTY EGFTTDLSVG
NTVLVDDGLI GMEVTAIEGN KVICKVLNNG DLGENKGVNL PGVSIALPAL AEKDKQDLIF
GCEQGVDFVA ASFIRKRSDV IEIREHLKAH GGENIHIISK IENQEGLNNF DEILEASDGI
MVARGDLGVE IPVEEVIFAQ KMMIEKCIRA RKVVITATQM LDSMIKNPRP TRAEAGDVAN
AILDGTDAVM LSGESAKGKY PLEAVSIMAT ICERTDRVMN SRLEFNNDNR KLRITEAVCR
GAVETAEKLD APLIVVATQG GKSARAVRKY FPDATILALT TNEKTAHQLV LSKGVVPQLV
KEITSTDDFY RLGKELALQS GLAHKGDVVV MVSGALVPSG TTNTASVHVL
Length

470

Mol. Wt

50.729 kDa

pI

6.0 (calculated)

Extinction coefficient

7,450 - 8,200 (calc based on 5 Y, 0 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Modification Site

36

phosphorylation site at S36

probability greater than 75%

PMID:17938405[8]

Domain

1..345

PF00224 Pyruvate kinase, barrel domain

PMID:19920124[9]

Domain

355..469

PF02887 Pyruvate kinase, alpha/beta domain

PMID:19920124[9]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pykF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129632

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946179

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005600

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AD61

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10804

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10804

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946179

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000795

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0797

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
  9. ↑ 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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