recF:Gene: Difference between revisions

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Revision as of 20:20, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recF

Mnemonic

Recombination

Synonyms

ECK3692, b3700, JW3677, uvrF[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.59 minutes, 83.59 minutes 

MG1655: 3879244..3878171
<gbrowseImage> name=NC_000913:3878171..3879244 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3841582..3840509
<gbrowseImage> name=NC_012967:3840509..3841582 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3766504..3767577
<gbrowseImage> name=NC_012759:3766504..3767577 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3759194..3760267
<gbrowseImage> name=AC_000091:3759194..3760267 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3976828..3975755
<gbrowseImage> name=NC_010473:3975755..3976828 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3878174

Edman degradation

PMID:2228960[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecF (Keio:JW3677)

deletion

deletion

PMID:16738554[3]

Shigen

recF::Tn5KAN-I-SceI (FB21402)

Insertion at nt 370 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21402

does not contain pKD46

recF::Tn5KAN-I-SceI (FB21403)

Insertion at nt 370 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21403

contains pKD46

recFK36R

K36R

Weakly active

seeded from UniProt:P0A7H0

recF143

CGSC:10318

recF400::Tn5

PMID:2841294[5]

CGSC:67402



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3677

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCCCTCACCCGCTTGTTGAT

Primer 2:CCATCCGTTATTTTACCCTTTTC

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[7]

est. P1 cotransduction: 87% [8]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[7]

est. P1 cotransduction: 9% [8]
Synonyms:zie-296::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10828

EchoBASE (EcoliWiki Page)

EchoBASE:EB0821

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3700

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4622

EcoGene (EcoliWiki Page)

EcoGene:EG10828

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RECF

CGSC (EcoliWiki Page)

CGSC:308

ASAP (EcoliWikiPage)

ASAP:ABE-0012095



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Griffin, TJ 4th & Kolodner, RD (1990) Purification and preliminary characterization of the Escherichia coli K-12 recF protein. J. Bacteriol. 172 6291-9 PubMed
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Thoms, B & Wackernagel, W (1988) Suppression of the UV-sensitive phenotype of Escherichia coli recF mutants by recA(Srf) and recA(Tif) mutations requires recJ+. J. Bacteriol. 170 3675-81 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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