recF:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
recF |
|---|---|
| Mnemonic |
Recombination |
| Synonyms |
ECK3692, b3700, JW3677, uvrF[1], uvrF |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
83.59 minutes, 83.59 minutes |
MG1655: 3879244..3878171 |
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NC_012967: 3841582..3840509 |
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NC_012759: 3766504..3767577 |
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W3110 |
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W3110: 3759194..3760267 |
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DH10B: 3976828..3975755 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3878174 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
recF(del) (Keio:JW3677) |
deletion |
deletion |
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recF::Tn5KAN-I-SceI (FB21402) |
Insertion at nt 370 in Plus orientation |
does not contain pKD46 | |||||
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recF::Tn5KAN-I-SceI (FB21403) |
Insertion at nt 370 in Plus orientation |
contains pKD46 | |||||
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recFK36R |
K36R |
Weakly active |
seeded from UniProt:P0A7H0 | ||||
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recF143 |
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recF400::Tn5 |
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recF |
deletion |
Sensitivity to |
decreases sensitivity to Bicyclomycin |
fig 2 | |||
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recF349 |
Growth Phenotype |
Mutations show approximately 50% decrease in viability relative to the wild type, table 1. |
JC18536 |
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recF4101 priA2:kan |
Growth Phenotype |
Double mutation decreases cell viability from wildtype, table 1. |
experimental strain: JC18991 |
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recF4115 priA2:kan |
Growth Phenotype |
Double mutation decreases cell viability, table 1. |
JC18996 |
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recF143 priA2::kan |
Growth Phenotype |
Double mutation causes decreased cell viability, table 1. |
JC18989 |
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recF143 |
Sensitivity to |
mutant shows attenuated UV-inducible SOS expression compared to wildtype, fig 1B. |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3677 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCCCTCACCCGCTTGTTGAT Primer 2:CCATCCGTTATTTTACCCTTTTC | |
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Kohara Phage |
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zid-501::Tn10 |
Linked marker |
est. P1 cotransduction: 87% [11] | |
|
Linked marker |
est. P1 cotransduction: 9% [11] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Griffin, TJ 4th & Kolodner, RD (1990) Purification and preliminary characterization of the Escherichia coli K-12 recF protein. J. Bacteriol. 172 6291-9 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Thoms, B & Wackernagel, W (1988) Suppression of the UV-sensitive phenotype of Escherichia coli recF mutants by recA(Srf) and recA(Tif) mutations requires recJ+. J. Bacteriol. 170 3675-81 PubMed
- ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
- ↑ 7.0 7.1 7.2 7.3 7.4 Sandler, SJ (1996) Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12. Mol. Microbiol. 19 871-80 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 CGSC: The Coli Genetics Stock Center
- ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
