cusF:Gene: Difference between revisions

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Revision as of 19:52, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cusF

Mnemonic

Cu sensor

Synonyms

ECK0565, b0573, JW0562, cusX, ylcC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

12.85 minutes 

MG1655: 596354..596686
<gbrowseImage> name=NC_000913:596354..596686 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 578034..578366
<gbrowseImage> name=NC_012967:578034..578366 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 499114..499446
<gbrowseImage> name=NC_012759:499114..499446 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 596354..596686
<gbrowseImage> name=AC_000091:596354..596686 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 535686..536018
<gbrowseImage> name=NC_010473:535686..536018 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 648946..649278
<gbrowseImage> name=NC_010473:648946..649278 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

596417

Edman degradation

PMID:12813074[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

cusFM71I

M71I

Loss of copper resistance and strong decrease in copper binding; when associated with I-69

seeded from UniProt:P77214

cusFF73Y

F73Y

No change in copper resistance

seeded from UniProt:P77214

cusFHH57QQ

HH57QQ

Slight decrease in copper resistance

seeded from UniProt:P77214

cusFM69I

M69I

Loss of copper resistance and strong decrease in copper binding; when associated with I-71

seeded from UniProt:P77214

cusFHHH25QQQ

HHH25QQQ

No change in copper resistance

seeded from UniProt:P77214

cusFD48N

D48N

No change in copper resistance

seeded from UniProt:P77214

ΔcusF (Keio:JW0562)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8687[4]

ΔcusF782::kan

PMID:16738554[3]

CGSC:100444


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0562

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAAAAGCACTGCAAGTCGC

Primer 2:CCCTGGCTGACTTTAATATCCTG

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[4]

est. P1 cotransduction: 14% [6]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[4]

est. P1 cotransduction: 19% [6]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6321

EchoBASE (EcoliWiki Page)

EchoBASE:EB3985

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0573

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0659

EcoGene (EcoliWiki Page)

EcoGene:EG14234

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YLCC

ASAP (EcoliWikiPage)

ASAP:ABE-0001967


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Franke, S et al. (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J. Bacteriol. 185 3804-12 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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