PMID:12813074
| Citation |
Franke, S, Grass, G, Rensing, C and Nies, DH (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J. Bacteriol. 185:3804-12 |
|---|---|
| Abstract |
The cus determinant of Escherichia coli encodes the CusCFBA proteins that mediate resistance to copper and silver by cation efflux. CusA and CusB were essential for copper resistance, and CusC and CusF were required for full resistance. Replacements of methionine residues 573, 623, and 672 with isoleucine in CusA resulted in loss of copper resistance, demonstrating their functional importance. Substitutions for several other methionine residues of this protein did not have any effect. The small 10-kDa protein CusF (previously YlcC) was shown to be a periplasmic protein. CusF bound one copper per polypeptide. The pink CusF copper protein complex exhibited an absorption maximum at around 510 nm. Methionine residues of CusF were involved in copper binding as shown by site-directed mutagenesis. CusF interacted with CusB and CusC polypeptides in a yeast two-hybrid assay. In contrast to other well-studied CBA-type heavy metal efflux systems, Cus was shown to be a tetrapartite resistance system that involves the novel periplasmic copper-binding protein CusF. These data provide additional evidence for the hypothesis that Cu(I) is directly transported from the periplasm across the outer membrane by the Cus complex. |
| Links | |
| Keywords |
Biological Transport; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Copper/metabolism; Copper/pharmacology; Drug Resistance, Bacterial; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Microbial Sensitivity Tests; Mutagenesis, Site-Directed; Operon; Periplasmic Proteins/chemistry; Periplasmic Proteins/genetics; Periplasmic Proteins/metabolism |
| edit table |
Significance
This paper demonstrates that several methionine residues of CusA are essential to the copper resistance phenotype. Also, it shows that CusF binds 1 Cu atoms per molecule of CusF.
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
