pykF:Gene: Difference between revisions
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10804 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10804 EcoCyc] | ||
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{{GBrowseLink|REL606|NC_012967|1732965|1734377|GBrowse (REL606)}} | {{GBrowseLink|REL606|NC_012967|1732965|1734377|GBrowse (REL606)}} | ||
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{{GBrowseLink|BW2952|012759|1645781|NC_1647193|GBrowse (BW2952)}} | {{GBrowseLink|BW2952|012759|1645781|NC_1647193|GBrowse (BW2952)}} | ||
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W3110: 1757412..1758824<br /> | W3110: 1757412..1758824<br /><gbrowseImage> | ||
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{{GBrowseLink|ecoli_W3110|AC_000091|1756412|1759824|GBrowse(W3110)}} | {{GBrowseLink|ecoli_W3110|AC_000091|1756412|1759824|GBrowse(W3110)}} | ||
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{{GBrowseLink|ecoli_DH10B|NC_010473|1841469|1848529|GBrowse(DH10B)}} | {{GBrowseLink|ecoli_DH10B|NC_010473|1841469|1848529|GBrowse(DH10B)}} | ||
Revision as of 21:36, 26 July 2010
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
pykF |
|---|---|
| Mnemonic |
Pyruvate kinase |
| Synonyms |
ECK1672, b1676, JW1666[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
37.8 minutes |
MG1655: 1753722..1755134 |
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NC_012967: 1732965..1734377 |
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NC_012759: 1645781..1647193 |
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|
W3110 |
|
W3110: 1757412..1758824 |
||
|
DH10B: 1844293..1845705 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1753722 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpykF (Keio:JW1666) |
deletion |
deletion |
|||||
|
At position 400 in Plus orientation, does not contain plasmid pKD46. | |||||||
|
At position 400 in Plus orientation, contains plasmid pKD46. | |||||||
|
ΔpykF751::kan |
| ||||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1666 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAAGACCAAAATTGTTTG Primer 2:CCaAGGACGTGAACAGATGCGGT | |
|
Linked marker |
est. P1 cotransduction: % [8] | ||
|
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: 42% [8] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Valentini, G et al. (1991) Bacterial pyruvate kinases have a shorter N-terminal domain. Biol. Chem. Hoppe-Seyler 372 91-3 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).