malF:Gene: Difference between revisions

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Revision as of 21:11, 26 July 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malF

Mnemonic

Maltose

Synonyms

ECK4025, b4033, JW3993[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.42 minutes, 91.42 minutes 

MG1655: 4243098..4241554
<gbrowseImage> name=NC_000913:4241554..4243098 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4224007..4222463
<gbrowseImage> name=NC_012967:4222463..4224007 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4180399..4181943
<gbrowseImage> name=NC_012759:4180399..4181943 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4248665..4247121
<gbrowseImage> name=AC_000091:4247121..4248665 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4342794..4341250
<gbrowseImage> name=NC_010473:4341250..4342794 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmalF (Keio:JW3993)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10874[3]

malFP420A

P420A

No effect

seeded from UniProt:P02916

malFG380C,S

G380C,S

No growth on maltose

seeded from UniProt:P02916

malFE401A,C,K,L

E401A,C,K,L

Reduction of transport rate

seeded from UniProt:P02916

malFS403C,D,K,L

S403C,D,K,L

Reduction of transport rate

seeded from UniProt:P02916

malFG407A,P

G407A,P

No effect

seeded from UniProt:P02916

malFL334W

L334W

Ability to transport lactose in a saturable manner

seeded from UniProt:P02916

malFL372W

L372W

Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose

seeded from UniProt:P02916

malFN376K,H

N376K,H

No growth on maltose

seeded from UniProt:P02916

malF3089::Tn10

Insertion at 4,242,342 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12164 = CGSC7476[3]

Synonyms:

malF64(Oc)

CGSC:7076

ochre (UAA) mutation

malF55::Tn10

CGSC:9952

malF59::Tn5

CGSC:9960

malF3089::Tn10

PMID:2540407[5]

CGSC:40369

malF3180::Tn10kan

PMID:2540407[5]

CGSC:40372

ΔmalF729::kan

PMID:16738554[2]

CGSC:101732


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3993

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGATGTCATTAAAAAGAAACA

Primer 2:CCATCAAACTTCATTCGCGTGGC

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 12% [4]
Synonyms:thi-39::Tn10

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[3]

est. P1 cotransduction: 3% [4]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10555

EchoBASE (EcoliWiki Page)

EchoBASE:EB0550

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4033

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5003

EcoGene (EcoliWiki Page)

EcoGene:EG10555

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MALF

CGSC (EcoliWiki Page)

CGSC:531

ASAP (EcoliWikiPage)

ASAP:ABE-0013195


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ 5.0 5.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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