fbp:Gene: Difference between revisions

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[[cgscmutation:100792|CGSC:100792]]
[[cgscmutation:100792|CGSC:100792]]
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Δfbp::kan
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deletion
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[[Biolog]]:respiration
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unable to respire [[Carbon sources|Glycerol]]
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PMID:16095938
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Revision as of 17:12, 26 April 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fbp

Mnemonic

Fructose-bisphosphatase

Synonyms

ECK4227, b4232, JW4191, fdp[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

95.97 minutes, 95.97 minutes 

MG1655: 4453632..4452634
gbrowse_img.php?coord=4452634..4453632&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4438859..4437861
gbrowse_img.php?coord=4437861..4438859&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4460289..4459291
gbrowse_img.php?coord=4459291..4460289&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4555440..4554442
gbrowse_img.php?coord=4554442..4555440&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 4391369..4392367
gbrowse_img.php?coord=4391369..4392367&genome=BW2952&name=NC_012759&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δfbp (Keio:JW4191)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11014[3]

Δfbp-287

CGSC:11279

fbp-5

CGSC:11723

Δfbp-779::kan

PMID:16738554[2]

CGSC:100792

Δfbp::kan

deletion

Biolog:respiration

unable to respire Glycerol

PMID:16095938[4]



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4191

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAACGTTAGGTGAATTTAT

Primer 2:CCgGCGTCCGGGAACTCACGGAT

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[3]

est. P1 cotransduction: 77% [6]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[3]

est. P1 cotransduction: % [6]
Synonyms:zjj-202::Tn10, zji-202::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10283

EchoBASE (EcoliWiki Page)

EchoBASE:EB0279

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4232

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5329

EcoGene (EcoliWiki Page)

EcoGene:EG10283

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:FBP

CGSC (EcoliWiki Page)

CGSC:784

ASAP (EcoliWikiPage)

ASAP:ABE-0013842



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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