putA:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="R46a9bdbd44f39" class=" tableEdit Gene_nomenclature_table" | ||
|- | |- | ||
!align=left {{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
|| | || | ||
''putA'' | ''putA'' | ||
|- | |- | ||
!align=left {{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
|| | || | ||
Proline utilization | |||
|- | |- | ||
!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
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''ECK1005, b1014, JW0999, poaA, putC, proline utilization A''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/> | ''ECK1005, b1014, JW0999, poaA, putC, proline utilization A''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/> | ||
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****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9bdbdbde7e" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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23.15 minutes | 23.15 minutes | ||
| | | | ||
MG1655: 1078105..1074143<br /> | MG1655: 1078105..1074143<br /><gbrowseImage> | ||
name=NC_000913:1074143..1078105 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10801 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10801 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|1073143|1079105|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|1074143|1078105|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10801 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10801 EcoCyc Sequence Dump] | ||
|- | |||
| | |||
[[REL606]] | |||
| | |||
| | |||
NC_012967: 1095166..1091204<br /><gbrowseImage> | |||
name=NC_012967:1091204..1095166 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|1091204|1095166|GBrowse (REL606)}} | |||
| | |||
{{GBrowseFastaLink|REL606|NC_012967|1091204|1095166|FASTA Dump}} | |||
|- | |||
| | |||
[[BW2952]] | |||
| | |||
| | |||
NC_012759: 977111..981073<br /><gbrowseImage> | |||
name=NC_012759:977111..981073 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|977111|981073|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|977111|981073|FASTA Dump}} | |||
|- | |- | ||
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W3110: 1079304..1075342<br />{{ | W3110: 1079304..1075342<br /><gbrowseImage> | ||
name=NC_007779:1075342..1079304 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|W3110|NC_007779|1074342|1080304|GBrowse(W3110)}} | |||
| | |||
{{GBrowseFastaLink|W3110|NC_007779|1075342|1079304|FASTA Dump}} | |||
|- | |||
| | |||
[[DH10B]] | |||
| | | | ||
| | | | ||
DH10B: 1132033..1128071<br /><gbrowseImage> | |||
name=NC_010473:1128071..1132033 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
|-class= | | | ||
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{{GBrowseFastaLink|DH10B|NC_010473|1120147|1139957|FASTA Dump}} | |||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="W46a9bdbe691c4" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V46a9bdbea9e57" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
| | | | ||
''putA(del)'' (Keio:JW0999) | |||
| | | | ||
deletion | deletion | ||
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PMID:16738554 | PMID:16738554 | ||
| | | | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | ||
[http://cgsc2.biology.yale.edu/Strain.php?ID=107262 CGSC8981]<ref name='LIB:cgsc'/> | |||
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'' | ''put(del)A758::kan'' | ||
| | | | ||
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|- | |||
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''putAP(del)101'' | |||
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| | |||
Auxotrophies | |||
| | |||
Proline Auxotroph | |||
| | |||
PMID:6355059 | |||
| | |||
Strains: RM2 | |||
| | |||
|- | |||
| | |||
''putP putA<sup> +</sup>'' | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Dehydroproline resistance | |||
| | |||
PMID:7016835 | |||
| | |||
Strain:CSH4 | |||
| | |||
table 2 | |||
|- | |||
| | |||
''putP putA<sup> +</sup>'' | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
L-Azetidine-2-carboxylate resistance | |||
| | |||
PMID:7016835 | |||
| | |||
Strain:CSH4 | |||
| | |||
table 2 | |||
|- | |||
| | |||
putP in strain C4 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Dehydroproline resistance | |||
| | |||
PMID:4865475 | |||
| | |||
Strain: C4 | |||
| | |||
Table 1 | |||
|- | |||
| | |||
putP in strain C4 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
L-Azetidine-2-carboxylate resistance | |||
| | |||
PMID:4865475 | |||
| | |||
Strain: C4 | |||
| | |||
Table 1 | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12587.J4e04c1d17f8cc--> | |||
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
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****************************************************************************************** -->{{Genetic_interaction_help}} | |||
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4e04c1d17f8cc" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.12587.J4e04c1d17f8cc&page=12587&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9bdbf550d2" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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Primer 2:ccACCTATAGTCATTAAGCTGGCGTTACCG | Primer 2:ccACCTATAGTCATTAAGCTGGCGTTACCG | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:7B5|7B5]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=228 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |- | ||
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R46a9bdbfdfd74" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[ | [[EcoCyc]] | ||
[ | |||
| | | | ||
EcoCyc:EG10801 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10801 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000792 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=945600 GeneID:945600] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0794 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0003424 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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==References== | ==References== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
{{RefHelp}} | |||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
[[Category:Gene List:MG1655]] | [[Category:Gene List:MG1655]] | ||
| Line 379: | Line 542: | ||
[[Category:Genes in the Keio knockout collection]] | [[Category:Genes in the Keio knockout collection]] | ||
[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:put genes]][[Category:Gene_List:DH10B]] | [[Category:put genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | ||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:31, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
putA |
|---|---|
| Mnemonic |
Proline utilization |
| Synonyms |
ECK1005, b1014, JW0999, poaA, putC, proline utilization A[1][2] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
23.15 minutes |
MG1655: 1078105..1074143 |
||
|
NC_012967: 1095166..1091204 |
||||
|
NC_012759: 977111..981073 |
||||
|
W3110 |
|
W3110: 1079304..1075342 |
||
|
DH10B: 1132033..1128071 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
putA(del) (Keio:JW0999) |
deletion |
deletion |
|||||
|
putA11 |
|||||||
|
putA1::Tn5 |
|||||||
|
put(del)A758::kan |
|||||||
|
putAP(del)101 |
Auxotrophies |
Proline Auxotroph |
Strains: RM2 |
||||
|
putP putA + |
Resistant to |
Dehydroproline resistance |
Strain:CSH4 |
table 2 | |||
|
putP putA + |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain:CSH4 |
table 2 | |||
|
putP in strain C4 |
Resistant to |
Dehydroproline resistance |
Strain: C4 |
Table 1 | |||
|
putP in strain C4 |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain: C4 |
Table 1 | |||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0999 |
Plasmid clone |
Status:Clone OK Primer 1:gccGGAACCACCACCATGGGGGTTAAGCTG Primer 2:ccACCTATAGTCATTAAGCTGGCGTTACCG | |
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 67% [10] | ||
|
Linked marker |
est. P1 cotransduction: % [10] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 Wood, JM (1981) Genetics of L-proline utilization in Escherichia coli. J. Bacteriol. 146 895-901 PubMed
- ↑ Stalmach, ME et al. (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156 481-6 PubMed
- ↑ 7.0 7.1 Tristram, H & Neale, S (1968) The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J. Gen. Microbiol. 50 121-37 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).