putA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
putA |
|---|---|
| Mnemonic |
Proline utilization |
| Synonyms |
ECK1005, b1014, JW0999, poaA, putC, proline utilization A[1][2] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
23.15 minutes |
MG1655: 1078105..1074143 |
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NC_012967: 1095166..1091204 |
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NC_012759: 977111..981073 |
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W3110 |
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W3110: 1079304..1075342 |
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DH10B: 1132033..1128071 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
putA(del) (Keio:JW0999) |
deletion |
deletion |
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putA11 |
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putA1::Tn5 |
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put(del)A758::kan |
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putAP(del)101 |
Auxotrophies |
Proline Auxotroph |
Strains: RM2 |
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putP putA + |
Resistant to |
Dehydroproline resistance |
Strain:CSH4 |
table 2 | |||
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putP putA + |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain:CSH4 |
table 2 | |||
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putP in strain C4 |
Resistant to |
Dehydroproline resistance |
Strain: C4 |
Table 1 | |||
|
putP in strain C4 |
Resistant to |
L-Azetidine-2-carboxylate resistance |
Strain: C4 |
Table 1 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0999 |
Plasmid clone |
Status:Clone OK Primer 1:gccGGAACCACCACCATGGGGGTTAAGCTG Primer 2:ccACCTATAGTCATTAAGCTGGCGTTACCG | |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 67% [10] | ||
|
Linked marker |
est. P1 cotransduction: % [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 Wood, JM (1981) Genetics of L-proline utilization in Escherichia coli. J. Bacteriol. 146 895-901 PubMed
- ↑ Stalmach, ME et al. (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156 481-6 PubMed
- ↑ 7.0 7.1 Tristram, H & Neale, S (1968) The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J. Gen. Microbiol. 50 121-37 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
