putA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PutA

Synonyms

fused DNA-binding transcriptional regulator[1], proline dehydrogenase[1], pyrroline-5-carboxylate dehydrogenase[1], B1014[2][1], PutC[2][1], PoaA[2][1], PutA[2][1] , ECK1005, JW0999, poaA, putC, b1014

Product description

PutA bifunctional enzyme and transcriptional regulator[2]; PutA transcriptional repressor, Proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase[3];

Component of PUTA-CPLX[2]; PutA transcriptional repressor, Proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase[3]

Proline dehydrogenase and repressor for the putAP divergon[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

PMID:1618807[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

PMID:18586269[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003842

1-pyrroline-5-carboxylate dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005933

F

Seeded from EcoCyc (v14.0)

complete

GO:0003842

1-pyrroline-5-carboxylate dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.5.1.12

F

Seeded from EcoCyc (v14.0)

complete

GO:0003842

1-pyrroline-5-carboxylate dehydrogenase activity

PMID:1618807[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0004657

proline dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002872

F

Seeded from EcoCyc (v14.0)

complete

GO:0004657

proline dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.5.99.8

F

Seeded from EcoCyc (v14.0)

complete

GO:0004657

proline dehydrogenase activity

PMID:1618807[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16606699[7]

IPI: Inferred from Physical Interaction

UniProtKB:P63389

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006537

glutamate biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002872

P

Seeded from EcoCyc (v14.0)

complete

GO:0006561

proline biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005933

P

Seeded from EcoCyc (v14.0)

complete

GO:0010133

proline catabolic process to glutamate

PMID:7006756[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0010843

promoter binding

PMID:18586269[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016566

specific transcriptional repressor activity

PMID:18586269[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0050660

FAD binding

PMID:1618807[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002872

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005933

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015590

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016160

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016161

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016162

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of PUTA-CPLX

could be indirect

Protein

ydaC

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

eutM

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

yfaS

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

dnaK

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

mukF

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

groL

PMID:16606699[7]

Experiment(s):EBI-1138642

Protein

rplQ

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

yfiD

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplR

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

ahpC

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

groS

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):2.778583

Protein

dnaN

PMID:19402753[9]

MALDI(Z-score):22.046630

Protein

elaB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpmC

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

hns

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

eno

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpmG

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsP

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

tufB

PMID:19402753[9]

MALDI(Z-score):18.820564

Protein

adk

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

phoA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

pgk

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):5.720428

Protein

rplI

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

fbaA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

hslV

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

ribE

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

cspC

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

talB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

leuD

PMID:19402753[9]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MGTTTMGVKL DDATRERIKS AATRIDRTPH WLIKQAIFSY LEQLENSDTL PELPALLSGA
ANESDEAPTP AEEPHQPFLD FAEQILPQSV SRAAITAAYR RPETEAVSML LEQARLPQPV
AEQAHKLAYQ LADKLRNQKN ASGRAGMVQG LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT
RDALIRDKIS NGNWQSHIGR SPSLFVNAAT WGLLFTGKLV STHNEASLSR SLNRIIGKSG
EPLIRKGVDM AMRLMGEQFV TGETIAEALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY
MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY PRLKSLTLLA
RQYDIGINID AEESDRLEIS LDLLEKLCFE PELAGWNGIG FVIQAYQKRC PLVIDYLIDL
ATRSRRRLMI RLVKGAYWDS EIKRAQMDGL EGYPVYTRKV YTDVSYLACA KKLLAVPNLI
YPQFATHNAH TLAAIYQLAG QNYYPGQYEF QCLHGMGEPL YEQVTGKVAD GKLNRPCRIY
APVGTHETLL AYLVRRLLEN GANTSFVNRI ADTSLPLDEL VADPVTAVEK LAQQEGQTGL
PHPKIPLPRD LYGHGRDNSA GLDLANEHRL ASLSSALLNS ALQKWQALPM LEQPVAAGEM
SPVINPAEPK DIVGYVREAT PREVEQALES AVNNAPIWFA TPPAERAAIL HRAAVLMESQ
MQQLIGILVR EAGKTFSNAI AEVREAVDFL HYYAGQVRDD FANETHRPLG PVVCISPWNF
PLAIFTGQIA AALAAGNSVL AKPAEQTPLI AAQGIAILLE AGVPPGVVQL LPGRGETVGA
QLTGDDRVRG VMFTGSTEVA TLLQRNIASR LDAQGRPIPL IAETGGMNAM IVDSSALTEQ
VVVDVLASAF DSAGQRCSAL RVLCLQDEIA DHTLKMLRGA MAECRMGNPG RLTTDIGPVI
DSEAKANIER HIQTMRSKGR PVFQAVRENS EDAREWQSGT FVAPTLIELD DFAELQKEVF
GPVLHVVRYN RNQLPELIEQ INASGYGLTL GVHTRIDETI AQVTGSAHVG NLYVNRNMVG
AVVGVQPFGG EGLSGTGPKA GGPLYLYRLL ANRPESALAV TLARQDAKYP VDAQLKAALT
QPLNALREWA ANRPELQALC TQYGELAQAG TQRLLPGPTG ERNTWTLLPR ERVLCIADDE
QDALTQLAAV LAVGSQVLWP DDALHRQLVK ALPSAVSERI QLAKAENITA QPFDAVIFHG
DSDQLRALCE AVAARDGTIV SVQGFARGES NILLERLYIE RSLSVNTAAA GGNASLMTIG
Length

1,320

Mol. Wt

143.817 kDa

pI

5.9 (calculated)

Extinction coefficient

124,110 - 125,735 (calc based on 39 Y, 12 W, and 13 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

654..1111

PF00171 Aldehyde dehydrogenase family

PMID:19920124[10]

Domain

228..574

PF01619 Proline dehydrogenase

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=putA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128980

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945600

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003424

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09546

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10801

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10801

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945600

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000792

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0794

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Brown, ED & Wood, JM (1992) Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli. J. Biol. Chem. 267 13086-92 PubMed
  6. ↑ 6.0 6.1 6.2 Zhou, Y et al. (2008) Structural basis of the transcriptional regulation of the proline utilization regulon by multifunctional PutA. J. Mol. Biol. 381 174-88 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ Wood, JM & Zadworny, D (1980) Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can. J. Biochem. 58 787-96 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 9.19 9.20 9.21 9.22 9.23 9.24 9.25 9.26 9.27 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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