ftsK:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|   id="D46a9bb0157a6c" class=" tableEdit Gene_nomenclature_table"


|-
|-
!align=left {{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''ftsK''  
''ftsK''
|-
|-
!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
filamentation temperature sensitive
Filamentation, temperature sensitive
|-
|-
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK0881, b0890, JW0873, dinH''<ref name='LIB:Riley_2006'/>
''ECK0881, b0890, JW0873, dinH''<ref name='LIB:Riley_2006'/>, dinH'',
usoM <ref name='PMID:9294449'/>


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*  
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****************************************************************************************** -->{{Gene_location_help}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9bb01cfcb0"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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20.1 minutes&nbsp;
20.1 minutes&nbsp;
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|
MG1655: 932447..936436<br />{{GBrowseFigureByCoord|931447|937436|ecoli|MG1655}}
MG1655: 932447..936436<br /><gbrowseImage>
name=NC_000913:932447..936436
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6464 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6464 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|931447|937436|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|931447|937436|GBrowse(MG1655)}}
   
   
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{{GBrowseFastaLink|ecoli|NC_000913|932447|936436|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|932447|936436|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G6464 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G6464 EcoCyc Sequence Dump]  
|-
|
[[REL606]]
|
|
NC_012967: 950282..954310<br /><gbrowseImage>
name=NC_012967:950282..954310
source=REL606
preset=GeneLocation
</gbrowseImage>
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{{GBrowseLink|REL606|NC_012967|950282|954310|GBrowse (REL606)}}
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{{GBrowseFastaLink|REL606|NC_012967|950282|954310|FASTA Dump}}
|-
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[[BW2952]]
|
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NC_012759: 835415..839404<br /><gbrowseImage>
name=NC_012759:835415..839404
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|NC_012759|835415|839404|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|NC_012759|835415|839404|FASTA Dump}}
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&nbsp;
&nbsp;
|
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W3110: 933646..937635<br />{{GBrowseFigureByCoord|932646|938635|ecoli_W3110|W3110}}
W3110: 933646..937635<br /><gbrowseImage>
name=NC_007779:933646..937635
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|932646|938635|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|932646|938635|GBrowse(W3110)}}  
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{{GBrowseFastaLink|W3110|NC_007779|933646|937635|FASTA Dump}}
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[[DH10B]]
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|933646|937635|FASTA Dump}}


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DH10B: 986375..990364<br /><gbrowseImage>
name=NC_010473:986375..990364
source=DH10B
preset=GeneLocation
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{{GBrowseLink|DH10B|NC_010473|978397|998342|GBrowse(DH10B)}}
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*  
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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9bb02b9f16"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
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|
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deltaLC
''LC(del)''
|
|


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|
|
 
|
|


|[[PMID:15522074]]<ref name=PMID:15522074/>
|
PMID:15522074
|
|


|
|
Carries a deletion of FtsK <sub>L</sub> and FtsK <sub>C</sub>  
Carries a deletion of FtsK <sub>L</sub> and FtsK <sub>C</sub>  
|-
|
''ftsK''G80A
|
|
G80A
|
|
In Toe44; loss of function under extreme conditions
|
|
|
seeded from [http://www.uniprot.org/uniprot/P46889 UniProt:P46889]
|-
|
ftsKE58A
|
|
E58A
|
|
Loss of function
|
|
|
seeded from [http://www.uniprot.org/uniprot/P46889 UniProt:P46889]
|-  
|-  
|
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|
|
 
|
|


|[[PMID:15522074]]<ref name=PMID:15522074/>
|
PMID:15522074
|
|
Barre
Barre
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|
|
 
|
|


|[[PMID:15522074]]<ref name=PMID:15522074/>
|
PMID:15522074
|
|
Barre
Barre
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K997A
K997A
|
|
 
|
|


|[[PMID:15522074]]<ref name=PMID:15522074/>
|
PMID:15522074
|
|
Barre
Barre
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|
|
 
|
|


|[[PMID:15522074]]<ref name=PMID:15522074/>
|
PMID:15522074
|
|
Barre
Barre
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|
|
 
|
 
|
|
only expresses the NTD due to a Cm resistance cassette at nt 678 of ''ftsK''
PMID:9721304
|[[PMID:9721304]]<ref name=PMID:9721304/>
|
|
Donachie
Donachie
Sheratt  
Sheratt  
|
only expresses the NTD due to a Cm resistance cassette at nt 678 of ''ftsK''
|-
|
''ftsk44''
|
missense mutation
|
G80A
|
Growth Phenotype
|
temperature sensitive late stage block in division, but does not affect chromosome replication or segregation
forms long filamentous cells at 42C
|
PMID:7592387
PMID:9721304
|
Donachie
|
this is the allele from the TOE44 strain listed in this table.
|-
|
''ftsK1::cat''
|
|
|
|
abnormal nucleoid segregation- asymmetrically positioned nucleoids and large anucleate regions
inhibited cell-cell separation
|
* PMID:9294448
* PMID:9829960
|
|
defect in the C-terminus of FtsK
|-
|
''ftsK1::cat mukB(del)''
|
|
|
Growth Phenotype
|
synthetically lethal
|
PMID:9829960
|
|
shown that it must be complemented in trans 
|-
|
''ftsK::cat-2''
|
Δ54-2201
|
|
|
|
PMID:9721304
|
|
almost the entire N-terminal membrane-spanning domain is deleted, and the C-terminal fragment is out of frame with the ''cat'' gene. Lethal- must be complemented ''in trans''
|-
|
''ftsK::cat-Δ5''
|
Δ54-3651
|
|
|
|
PMID:9721304
|
|
this is a deletion of 90% of ''ftsK''
|-
|
''ftsK3531''
|
missense mutation
|
|
Growth Phenotype
|
same phenotype as ''ftsK44''- temperature sensitive late stage block in division, but does not affect chromosome replication or segregation
|
PMID:9721304
|
|
suppressed by high salt (1% NaCl) in the medium or by insertional inactivation of the ''dacA'' gene, encoding PBP5.
|-
|
''ftsK::cat-2(del) dacA(del)::kan''
|
|
|
Cell Shape
|
*forms small colonies on plates grows well
*produces a population of misshapen cells in liquid culture
|
PMID:9721304
|
|
|-
|
''ftsK::cat-5(del) dac(del)::kan''
|
|
|
|
synthetically lethal
|
PMID:9721304
|


|
|
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|-  
|-  
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ftsk44
''ftsK<sub>C</sub> priA2::kan''
|
|


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Growth Phenotype
Growth Phenotype
|
|
temperature sensitive late stage block in division, but does not affect chromosome replication or segregation
synthetically lethal
|[[PMID:7592387]]<ref name=PMID:7592387/>
|
PMID:11459954
 
|
 
|
|-
|
''ftsK1::cat minB(del)''
|
 
|
 
|
Growth Phenotype
|
chains of minicells generated (many of which contained DNA)
|
PMID:9829960
|
 
|
|-
|
''ftsK100''
|
 
|
K997A
|
 
|
ATP mutant
|
PMID:18363794
|
 
|
|-
|
ftsK10
|
 
|
 
|
Growth Phenotype
|
affects expression form the uspA promoter, results in salt sensitivity, stationary-phase survival is impaired, and cell-cell separation is blocked
|
PMID:9294449
|
 
|
Also called usoM10
|-
|
ftsK
|
deletion in the c-terminal
|
 
|
Growth Phenotype
|
inhibits FtsK activity and increases recombination near dif
|
PMID:15916604
PMID:9294449
|
|
Donachie
 
|
|
   
   


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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===
Deletions that leave the membrane segments intact are viable.<ref name='PMID: 9721304'/>
Deletions that leave the membrane segments intact are viable<ref name='PMID: 9721304'/>. Suppressors of the ''ftsK'' chaining/filamentation phenotype are discussed in Geissler and Margolin (2005)<ref name='PMID:16194242'/>.
 
 
==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12339.X4e04bb9e6c34f-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X4e04bb9e6c34f"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
 
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<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R46a9bb0364216"  class=" tableEdit Gene_resource_table"   
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|[[PMID:16769691]]<ref name=PMID:16769691/>
|
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCGTCAAACGGCGGTGGGGCCAG  
Primer 2:CCGTCAAACGGCGGTGGGGCCAG  
|-
|
[[:Category:Kohara_Phage:1F10|1F10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=214 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:E6H3|E6H3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=215 Genobase]
|
PMID:3038334
|-  
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|
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est. P1 cotransduction: 61% <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: 61% <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:zbj-1230::Tn10  
Synonyms:zbj-1230::Tn10  
|-
|
W3110::P''lac-ftsK''
|
Strain
|
|
Depletion Strain<ref name='PMID:9723913'/> 
|-
|
pWL71
|
Plasmid Clone
|
Pbad-FtsK(1-202)-GFP
|
Successfully complements the W3110::P''lac-ftsK'' depletion strain<ref name='PMID:9723913'/>
|-
|
pWL72
|
Plasmid Clone
|
Pbad-FtsK(1-211)-GFP
|
Successfully complements the W3110::P''lac-ftsK'' depletion strain <ref name='PMID:9723913'/> 
|-
|
pWL73
|
Plasmid Clone
|
Pbad-FtsK(1-251)-GFP
|
Successfully complements the W3110::P''lac-ftsK'' depletion strain <ref name='PMID:9723913'/> 
|-
|
pWL74
|
Plasmid Clone
|
Pbad-FtsK(1-644)
|
Successfully complements the W3110::P''lac-ftsK'' depletion strain <ref name='PMID:9723913'/> 
|-
|
pWL75
|
Plasmid Clone
|
Pbad-FtsK(1-1329)
|
Successfully complements the W3110::P''lac-ftsK'' depletion strain <ref name='PMID:9723913'/> 
|-
|
pWL76
|
Plasmid Clone
|
Pbad-FtsK(1-19 and 637-1329)- N-terminal 19 amino acids fused in phase to amino acid residue 637 of FtsK
|
Cannot complement the W3110::P''lac-ftsK'' depletion strain <ref name='PMID:9723913'/> 
|-
|
pFtsK-PhoA plasmids
|
Plasmid Clone
|
|
This represents multiple plasmids with ''phoA'' inserted at various positions<ref name='PMID:10922461'/>.
|-
|
pFtsK-LacZ plasmids
|
Plasmid Clone
|


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This represents multiple plasmids with ''lacZ'' inserted at various positions<ref name='PMID:10922461'/>.
|-
|
pFLAG-FtsK plasmids
|
Plasmid Clone
|
 
|
This reference describes plasmids with various FtsK truncations N-terminally tagged to FLAG<ref name='PMID:11114887'/>.
|-
|
MA3
|
Strain
|
AB1157''ftsK''
|
PMID:10523315
|-
|
GR51
|
Strain
|
AB1157''xerCftsK''
|
PMID:10523315
|-
|
GR59
|
Strain
|
AB1157''ftsK recA56 srl::Tn10''
|
PMID:10523315
|-
|
FC1
|
Strain
|
AB1157, ''ftsK100'' K997A (ATP mutant), Cm<sup>R</sup>
|
PMID:18363794
|-
|
FtsK-YFP
|
Plasmid Clone
|
*low-copy expression vectors derived from pSC101
*Spectinomycin resistance gene
|
PMID:18363794
|-
|
FtsKΔγ
|
Plasmid Clone
|
FtsK<sub>50C</sub> deleted from residue 1248
|
PMID:19854947
 
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6464 EcoCyc:G6464]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3016 EchoBASE:EB3016]
EcoCyc:G6464
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0890 EcoliGenExpDB:b0890]
EcoGene:EG13226
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
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[http://BioCyc.org/ECOL199310/new-image?object=C1027 ECOL199310CYC:C1027]
RegulonDB:ECK120003064
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG13226 EcoGene:EG13226]
[http://www.ncbi.nlm.nih.gov/gene?term=945102 GeneID:945102]
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Escherichia coli str. K-12 substr. MG1655
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
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[http://BioCyc.org/ECOO157/new-image?object=FTSK ECOO157CYC:FTSK]
EchoBASE:EB3016
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003027&formSubmitReturn=1 ASAP:ABE-0003027]
ASAP:ABE-0003027
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
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===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:fts genes]]
[[Category:fts genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:32, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ftsK

Mnemonic

filamentation temperature sensitive Filamentation, temperature sensitive

Synonyms

ECK0881, b0890, JW0873, dinH[1], dinH, usoM [2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

20.1 minutes 

MG1655: 932447..936436
<gbrowseImage> name=NC_000913:932447..936436 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 950282..954310
<gbrowseImage> name=NC_012967:950282..954310 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 835415..839404
<gbrowseImage> name=NC_012759:835415..839404 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 933646..937635
<gbrowseImage> name=NC_007779:933646..937635 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 986375..990364
<gbrowseImage> name=NC_010473:986375..990364 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

LC(del)

PMID:15522074[3]

Carries a deletion of FtsK L and FtsK C

ftsKG80A

G80A

In Toe44; loss of function under extreme conditions

seeded from UniProt:P46889

ftsKE58A

E58A

Loss of function

seeded from UniProt:P46889

NC

PMID:15522074[3]

Barre

Deletion of the FtsK Linker

NL hi C

PMID:15522074[3]

Barre

Carries swaps b/t E.coli and H. influenzae FtsK regions

ftsK A

K997A

PMID:15522074[3]

Barre

Carries a single point mutation in the walker A motif of FtsK C

NLC hi

PMID:15522074[3]

Barre

Carries swaps b/t E.coli and H.influenzae FtsK regions

ftsk::cat1

PMID:9721304[4]

Donachie Sheratt

only expresses the NTD due to a Cm resistance cassette at nt 678 of ftsK

ftsk44

missense mutation

G80A

Growth Phenotype

temperature sensitive late stage block in division, but does not affect chromosome replication or segregation

forms long filamentous cells at 42C

PMID:7592387[5]

PMID:9721304[4]

Donachie

this is the allele from the TOE44 strain listed in this table.

ftsK1::cat

abnormal nucleoid segregation- asymmetrically positioned nucleoids and large anucleate regions

inhibited cell-cell separation

defect in the C-terminus of FtsK

ftsK1::cat mukB(del)

Growth Phenotype

synthetically lethal

PMID:9829960[7]

shown that it must be complemented in trans

ftsK::cat-2

Δ54-2201

PMID:9721304[4]

almost the entire N-terminal membrane-spanning domain is deleted, and the C-terminal fragment is out of frame with the cat gene. Lethal- must be complemented in trans

ftsK::cat-Δ5

Δ54-3651

PMID:9721304[4]

this is a deletion of 90% of ftsK

ftsK3531

missense mutation

Growth Phenotype

same phenotype as ftsK44- temperature sensitive late stage block in division, but does not affect chromosome replication or segregation

PMID:9721304[4]

suppressed by high salt (1% NaCl) in the medium or by insertional inactivation of the dacA gene, encoding PBP5.

ftsK::cat-2(del) dacA(del)::kan

Cell Shape

  • forms small colonies on plates grows well
  • produces a population of misshapen cells in liquid culture

PMID:9721304[4]

ftsK::cat-5(del) dac(del)::kan

synthetically lethal

PMID:9721304[4]

ftsKC priA2::kan

Growth Phenotype

synthetically lethal

PMID:11459954[8]

ftsK1::cat minB(del)

Growth Phenotype

chains of minicells generated (many of which contained DNA)

PMID:9829960[7]

ftsK100

K997A

ATP mutant

PMID:18363794[9]

ftsK10

Growth Phenotype

affects expression form the uspA promoter, results in salt sensitivity, stationary-phase survival is impaired, and cell-cell separation is blocked

PMID:9294449[2]

Also called usoM10

ftsK

deletion in the c-terminal

Growth Phenotype

inhibits FtsK activity and increases recombination near dif

PMID:15916604[10] PMID:9294449[2]


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Notes

Deletions that leave the membrane segments intact are viable[11]. Suppressors of the ftsK chaining/filamentation phenotype are discussed in Geissler and Margolin (2005)[12].


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0873

Plasmid clone

Shigen

PMID:16769691[13]

Status:Clone OK

Primer 1:GCCAGCCAGGAATACATTGAAGA

Primer 2:CCGTCAAACGGCGGTGGGGCCAG

1F10

Kohara Phage

Genobase

PMID:3038334[14]

E6H3

Kohara Phage

Genobase

PMID:3038334[14]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[15]

est. P1 cotransduction: % [16]
Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[15]

est. P1 cotransduction: 61% [16]
Synonyms:zbj-1230::Tn10

W3110::Plac-ftsK

Strain

Depletion Strain[17]

pWL71

Plasmid Clone

Pbad-FtsK(1-202)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain[17]

pWL72

Plasmid Clone

Pbad-FtsK(1-211)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL73

Plasmid Clone

Pbad-FtsK(1-251)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL74

Plasmid Clone

Pbad-FtsK(1-644)

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL75

Plasmid Clone

Pbad-FtsK(1-1329)

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL76

Plasmid Clone

Pbad-FtsK(1-19 and 637-1329)- N-terminal 19 amino acids fused in phase to amino acid residue 637 of FtsK

Cannot complement the W3110::Plac-ftsK depletion strain [17]

pFtsK-PhoA plasmids

Plasmid Clone

This represents multiple plasmids with phoA inserted at various positions[18].

pFtsK-LacZ plasmids

Plasmid Clone

This represents multiple plasmids with lacZ inserted at various positions[18].

pFLAG-FtsK plasmids

Plasmid Clone

This reference describes plasmids with various FtsK truncations N-terminally tagged to FLAG[19].

MA3

Strain

AB1157ftsK

PMID:10523315[20]

GR51

Strain

AB1157xerCftsK

PMID:10523315[20]

GR59

Strain

AB1157ftsK recA56 srl::Tn10

PMID:10523315[20]

FC1

Strain

AB1157, ftsK100 K997A (ATP mutant), CmR

PMID:18363794[9]

FtsK-YFP

Plasmid Clone

  • low-copy expression vectors derived from pSC101
  • Spectinomycin resistance gene

PMID:18363794[9]

FtsKΔγ

Plasmid Clone

FtsK50C deleted from residue 1248

PMID:19854947[21]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6464

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13226

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003064

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945102

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3016

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003027

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 Arnau, J et al. (1997) Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase. J. Bacteriol. 179 5884-91 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 3.4 Bigot, S et al. (2004) FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol. Microbiol. 54 876-86 PubMed
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
  5. ↑ Begg, KJ et al. (1995) A new Escherichia coli cell division gene, ftsK. J. Bacteriol. 177 6211-22 PubMed
  6. ↑ Diez, AA et al. (1997) A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA. J. Bacteriol. 179 5878-83 PubMed
  7. ↑ 7.0 7.1 7.2 Yu, XC et al. (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol. 180 6424-8 PubMed
  8. ↑ McCool, JD & Sandler, SJ (2001) Effects of mutations involving cell division, recombination, and chromosome dimer resolution on a priA2::kan mutant. Proc. Natl. Acad. Sci. U.S.A. 98 8203-10 PubMed
  9. ↑ 9.0 9.1 9.2 Kennedy, SP et al. (2008) Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli. Mol. Microbiol. 68 1018-28 PubMed
  10. ↑ Corre, J & Louarn, JM (2005) Extent of the activity domain and possible roles of FtsK in the Escherichia coli chromosome terminus. Mol. Microbiol. 56 1539-48 PubMed
  11. ↑ Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
  12. ↑ Geissler, B & Margolin, W (2005) Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol. Microbiol. 58 596-612 PubMed
  13. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  14. ↑ 14.0 14.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  15. ↑ 15.0 15.1 CGSC: The Coli Genetics Stock Center
  16. ↑ 16.0 16.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  17. ↑ 17.0 17.1 17.2 17.3 17.4 17.5 17.6 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed
  18. ↑ 18.0 18.1 Dorazi, R & Dewar, SJ (2000) Membrane topology of the N-terminus of the Escherichia coli FtsK division protein. FEBS Lett. 478 13-8 PubMed
  19. ↑ Barre, FX et al. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14 2976-88 PubMed
  20. ↑ 20.0 20.1 20.2 Recchia, GD et al. (1999) FtsK-dependent and -independent pathways of Xer site-specific recombination. EMBO J. 18 5724-34 PubMed
  21. ↑ Graham, JE et al. (2010) FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 38 72-81 PubMed

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