ftsK:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
FtsK |
|---|---|
| Synonyms |
DNA-binding membrane protein required for chromosome resolution and partitioning[1], B0890[2][1], DinH[2][1], FtsK[2][1] , dinH, ECK0881, JW0873, b0890 |
| Product description |
essential cell division protein FtsK [3][2][4]; Component of Xer site-specific recombination system[4][5] DNA translocase at septal ring sorting daughter chromsomes[6][5] Couples cell division and chromosome segregation. [7] Required at an early step in septation. [3] |
| EC number (for enzymes) |
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| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0051301 |
cell division |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0007059 |
chromosome segregation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0051301 |
cell division |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
IMP: Inferred from Mutant Phenotype |
P |
expression of the cytoplasmic domain of FtsK facilitates chromosome segregation in parC(ts) and parE(ts) cells |
complete | |||
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005886 |
plasma membrane |
EXP: Inferred from Experiment |
C |
complete | ||||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051301 |
cell division |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006970 |
response to osmotic stress |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0005887 |
integral to plasma membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0003677 |
DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0007049 |
cell cycle |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0007049 |
cell cycle |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0007059 |
chromosome segregation |
IGI: Inferred from Genetic Interaction |
UniProtKB:P0A734 UniProtKB:P0AEZ3 UniProtKB:P18196
|
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015616 |
DNA translocase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0043565 |
sequence-specific DNA binding |
IMP: Inferred from Mutant Phenotype |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0043565 |
sequence-specific DNA binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0045941 |
positive regulation of transcription |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0071236 |
cellular response to antibiotic |
IMP: Inferred from Mutant Phenotype |
P |
mitomycin C |
complete | |||
| GO:0007059 |
chromosome segregation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0015616 |
DNA translocase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0007059 |
chromosome segregation |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0051301 |
cell division |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0007059 |
chromosome segregation |
IPI: Inferred from Physical Interaction |
UniProtKB:P0A8P6 UniProtKB:P0A8P8
|
P |
complete | |||
| GO:0045893 |
positive regulation of transcription, DNA-dependent |
IMP: Inferred from Mutant Phenotype |
P |
increases PuspA activity in stationary phase |
complete | |||
|
Contributes to |
GO:0006265 |
DNA topological change |
IDA: Inferred from Direct Assay |
P |
stimulates Topo IV's ability to relax positively supercoiled DNA |
complete | ||
| GO:0009651 |
response to salt stress |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
|
Contributes to |
GO:0007059 |
chromosome segregation |
IDA: Inferred from Direct Assay |
P |
stimulates Topo IV's decatenation ability |
complete | ||
| GO:0000920 |
cytokinetic cell separation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0007059 |
chromosome segregation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0007059 |
chromosome segregation |
IGI: Inferred from Genetic Interaction |
UniProtKB:P0A734 UniProtKB:P0AEZ3 UniProtKB:P18196
|
P |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of Xer site-specific recombination system |
could be indirect |
||
|
Protein |
aceE |
Experiment(s):EBI-888719 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):99.6 | ||
|
Protein |
yfiD |
LCMS(ID Probability):99.6 | ||
|
Protein |
ParC subunit of Topo IV |
|||
|
Protein |
Either subunit of the XerCD Recombinase |
Triplex Displacement Assays | ||
|
Protein |
FtsZ and FtsA |
FtsK localization depends on FtsZ and FtsA prior localization |
| |
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
plasma membrane |
C-terminus localized in the cytoplasm with 6 predicted transmembrane domains |
Daley et al. (2005) [23] |
||
|
Inner membrane |
GFP fusion localization |
Signal sequence in the N-terminal domain of the protein |
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|
Inner membrane |
PhoA and beta-gal assays |
|||
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Cytosol |
FtsK (818-1329) |
Microscopy |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSQEYIEDKE VTLTKLSSGR RLLEALLILI VLFAVWLMAA LLSFNPSDPS WSQTAWHEPI HNLGGMPGAW LADTLFFIFG VMAYTIPVII VGGCWFAWRH QSSDEYIDYF AVSLRIIGVL ALILTSCGLA AINADDIWYF ASGGVIGSLL STTLQPLLHS SGGTIALLCV WAAGLTLFTG WSWVTIAEKL GGWILNILTF ASNRTRRDDT WVDEDEYEDD EEYEDENHGK QHESRRARIL RGALARRKRL AEKFINPMGR QTDAALFSGK RMDDDEEITY TARGVAADPD DVLFSGNRAT QPEYDEYDPL LNGAPITEPV AVAAAATTAT QSWAAPVEPV TQTPPVASVD VPPAQPTVAW QPVPGPQTGE PVIAPAPEGY PQQSQYAQPA VQYNEPLQQP VQPQQPYYAP AAEQPAQQPY YAPAPEQPVA GNAWQAEEQQ STFAPQSTYQ TEQTYQQPAA QEPLYQQPQP VEQQPVVEPE PVVEETKPAR PPLYYFEEVE EKRAREREQL AAWYQPIPEP VKEPEPIKSS LKAPSVAAVP PVEAAAAVSP LASGVKKATL ATGAAATVAA PVFSLANSGG PRPQVKEGIG PQLPRPKRIR VPTRRELASY GIKLPSQRAA EEKAREAQRN QYDSGDQYND DEIDAMQQDE LARQFAQTQQ QRYGEQYQHD VPVNAEDADA AAEAELARQF AQTQQQRYSG EQPAGANPFS LDDFEFSPMK ALLDDGPHEP LFTPIVEPVQ QPQQPVAPQQ QYQQPQQPVP PQPQYQQPQQ PVAPQPQYQQ PQQPVAPQQQ YQQPQQPVAP QQQYQQPQQP VAPQPQDTLL HPLLMRNGDS RPLHKPTTPL PSLDLLTPPP SEVEPVDTFA LEQMARLVEA RLADFRIKAD VVNYSPGPVI TRFELNLAPG VKAARISNLS RDLARSLSTV AVRVVEVIPG KPYVGLELPN KKRQTVYLRE VLDNAKFRDN PSPLTVVLGK DIAGEPVVAD LAKMPHLLVA GTTGSGKSVG VNAMILSMLY KAQPEDVRFI MIDPKMLELS VYEGIPHLLT EVVTDMKDAA NALRWCVNEM ERRYKLMSAL GVRNLAGYNE KIAEADRMMR PIPDPYWKPG DSMDAQHPVL KKEPYIVVLV DEFADLMMTV GKKVEELIAR LAQKARAAGI HLVLATQRPS VDVITGLIKA NIPTRIAFTV SSKIDSRTIL DQAGAESLLG MGDMLYSGPN STLPVRVHGA FVRDQEVHAV VQDWKARGRP QYVDGITSDS ESEGGAGGFD GAEELDPLFD QAVQFVTEKR KASISGVQRQ FRIGYNRAAR IIEQMEAQGI VSEQGHNGNR EVLAPPPFD |
| Length |
1,329 |
| Mol. Wt |
146.661 kDa |
| pI |
4.8 (calculated) |
| Extinction coefficient |
173,040 - 173,540 (calc based on 46 Y, 19 W, and 4 C residues) |
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Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/>
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Structure
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Structure figures<protect> | ||||||
Notes
- Membrane topology studies using phoA and lacZ fusions are described in Dorazi et al.(2000)[11]
- Crystal structure of P. aeruginosa FtsK complexed with KOPS with a putative molecular mechanism for loading and translocation may be found in Lowe et al. (2008)[13].
- FtsK links chromosome segregation to septation via its domain structure. The N-terminal domain is required for septation and its C-terminal domain is required for chromosome segregation[27].
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
|
Antibody |
raised against FtsK(447-729) |
isolated from rabbit [3] |
|
Purification protocol |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed
- ↑ 4.0 4.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Capiaux, H et al. (2002) A dual role for the FtsK protein in Escherichia coli chromosome segregation. EMBO Rep. 3 532-6 PubMed
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Robertson, S (1992) Putting pen to paper. Aust Crit Care 5 2 PubMed
- ↑ Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
- ↑ Begg, KJ et al. (1995) A new Escherichia coli cell division gene, ftsK. J. Bacteriol. 177 6211-22 PubMed
- ↑ Grainge, I et al. (2007) Unlinking chromosome catenanes in vivo by site-specific recombination. EMBO J. 26 4228-38 PubMed
- ↑ 11.0 11.1 11.2 11.3 11.4 Dorazi, R & Dewar, SJ (2000) Membrane topology of the N-terminus of the Escherichia coli FtsK division protein. FEBS Lett. 478 13-8 PubMed
- ↑ 12.0 12.1 12.2 12.3 12.4 12.5 Diez, AA et al. (1997) A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA. J. Bacteriol. 179 5878-83 PubMed
- ↑ 13.0 13.1 13.2 13.3 Löwe, J et al. (2008) Molecular mechanism of sequence-directed DNA loading and translocation by FtsK. Mol. Cell 31 498-509 PubMed
- ↑ Levy, O et al. (2005) Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase. Proc. Natl. Acad. Sci. U.S.A. 102 17618-23 PubMed
- ↑ Ptacin, JL et al. (2006) Identification of the FtsK sequence-recognition domain. Nat. Struct. Mol. Biol. 13 1023-5 PubMed
- ↑ Sivanathan, V et al. (2006) The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS. Nat. Struct. Mol. Biol. 13 965-72 PubMed
- ↑ 17.0 17.1 Yu, XC et al. (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol. 180 6424-8 PubMed
- ↑ 18.0 18.1 18.2 18.3 18.4 Graham, JE et al. (2010) FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 38 72-81 PubMed
- ↑ 19.0 19.1 19.2 Bigot, S & Marians, KJ (2010) DNA chirality-dependent stimulation of topoisomerase IV activity by the C-terminal AAA+ domain of FtsK. Nucleic Acids Res. 38 3031-40 PubMed
- ↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 21.0 21.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 22.0 22.1 Espeli, O et al. (2003) A physical and functional interaction between Escherichia coli FtsK and topoisomerase IV. J. Biol. Chem. 278 44639-44 PubMed
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Yu, XC et al. (1998) Localization of cell division protein FtsK to the Escherichia coli septum and identification of a potential N-terminal targeting domain. J. Bacteriol. 180 1296-304 PubMed
- ↑ Barre, FX et al. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14 2976-88 PubMed
- ↑ 26.0 26.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
- ↑ Bigot, S et al. (2007) FtsK, a literate chromosome segregation machine. Mol. Microbiol. 64 1434-41 PubMed
- ↑ Aussel, L et al. (2002) FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases. Cell 108 195-205 PubMed
Categories
- GO:0051301 ! cell division
- GO:0007059 ! chromosome segregation
- GO:0000166 ! nucleotide binding
- GO:0005886 ! plasma membrane
- GO:0005524 ! ATP binding
- GO:0006970 ! response to osmotic stress
- GO:0005887 ! integral component of plasma membrane
- GO:0003677 ! DNA binding
- GO:0007049 ! cell cycle
- GO:0016887 ! ATPase activity
- GO:0015616 ! DNA translocase activity
- GO:0043565 ! sequence-specific DNA binding
- GO:0045941 ! positive regulation of transcription
- GO:0016020 ! membrane
- GO:0071236 ! cellular response to antibiotic
- GO:0045893 ! positive regulation of transcription, DNA-templated
- GO:0006265 ! DNA topological change
- GO:0009651 ! response to salt stress
- GO:0000920 ! septum digestion after cytokinesis
- TMHMM Prediction
- Proteins
