PMID:9829960
| Citation |
Yu, XC, Weihe, EK and Margolin, W (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol. 180:6424-8 |
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| Abstract |
FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null mutation resulted in a synthetic lethal phenotype. When the truncated ftsK was combined with a minCDE deletion, chains of minicells were generated, many of which contained DNA. These results suggest that the C terminus of FtsK has an important role in chromosome partitioning. |
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| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Cell Division/genetics; Cell Division/physiology; Chromosomes, Bacterial/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Genes, Bacterial; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/physiology; Mutation; Phenotype; Sequence Deletion |
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Significance
This paper presents data suggesting that:
- the FtsK CTD is involved in chromosome positioning
- the FtsK CTD and MukB have redundant functions due to the synthetically lethal phenotype.
Useful Materials and Methods
Cephalexin is used to induce filamentation (by inhibiting FtsI). Chromosome segregation was easier to study in filamented cells.
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