PMID:19854947
| Citation |
Graham, JE, Sivanathan, V, Sherratt, DJ and Arciszewska, LK (2010) FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 38:72-81 |
|---|---|
| Abstract |
Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes. |
| Links |
PubMed PMC2800217 Online version:10.1093/nar/gkp843 |
| Keywords |
Adenosine Triphosphatases/metabolism; Binding Sites; DNA/metabolism; DNA Cleavage; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Integrases/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Protein Structure, Tertiary; Protein Transport; Recombination, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
