cusF:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="W46a9b3c362336"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''cusF''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''cusF''
Cu sensor
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK0565, b0573, JW0562, cusX, ylcC''<ref name='LIB:Riley_2006'/>, silF, ylcC''


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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.11707.W46a9b3c362336&page=11707&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK0565, b0573, JW0562, cusX, ylcC''<ref name='LIB:Riley_2006'/>
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===Notes===
===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46a9b3c3da0de"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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12.85 minutes&nbsp;
12.85 minutes&nbsp;
|
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MG1655: 596354..596686<br />{{GBrowseFigureByCoord|595354|597686|ecoli|MG1655}}
MG1655: 596354..596686<br /><gbrowseImage>
name=NC_000913:596354..596686
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6321 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6321 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:595354..597686 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|595354|597686|GBrowse(MG1655)}}
   
   
|
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:596354..596686&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|596354|596686|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G6321 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G6321 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 578034..578366<br /><gbrowseImage>
name=NC_012967:578034..578366
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|578034|578366|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|578034|578366|FASTA Dump}}
|-
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[[BW2952]]
|
 
|
NC_012759: 499114..499446<br /><gbrowseImage>
name=NC_012759:499114..499446
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|499114|499446|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|499114|499446|FASTA Dump}}
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 596354..596686<br />{{GBrowseFigureByCoord|595354|597686|ecoli_W3110|W3110}}  
W3110: 596354..596686<br /><gbrowseImage>
name=NC_007779:596354..596686
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|595354|597686|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|596354|596686|FASTA Dump}}  
|-
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[[DH10B]]
|
 
|
DH10B: 535686..536018<br /><gbrowseImage>
name=NC_010473:535686..536018
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|535022|536682|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|535022|536682|FASTA Dump}}
|-
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[[DH10B]]
|
 
|
DH10B: 648946..649278<br /><gbrowseImage>
name=NC_010473:648946..649278
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:595354..597686 GBrowse(W3110)]
{{GBrowseLink|DH10B|NC_010473|648282|649942|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:596354..596686&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|648282|649942|FASTA Dump}}
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
596417
|
Edman degradation
|
PMID:12813074<br />
|
 
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F46a9b3c4c2e82"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
cusFM71I
|
|
M71I
|
|
Loss of copper resistance and strong decrease in copper binding; when associated with I-69
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-
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cusFF73Y
|
|
F73Y
|
|
No change in copper resistance
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-
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cusFHH57QQ
|
|
HH57QQ
|
|
Slight decrease in copper resistance
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-
|
cusFM69I
|
|
M69I
|
|
Loss of copper resistance and strong decrease in copper binding; when associated with I-71
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-
|
cusFHHH25QQQ
|
|
HHH25QQQ
|
|
No change in copper resistance
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-
|
cusFD48N
|
|
D48N
|
|
No change in copper resistance
|
|
|
seeded from [http://www.uniprot.org/uniprot/P77214 UniProt:P77214]
|-  
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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106968 CGSC8687]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106968 CGSC8687]<ref name='LIB:cgsc'/>  
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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
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[[cgscmutation:100444|CGSC:100444]]
[[cgscmutation:100444|CGSC:100444]]
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.11707.F46a9b3c4c2e82&page=11707&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || ||
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW0562
JW0562
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
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[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCCTGGCTGACTTTAATATCCTG  
Primer 2:CCCTGGCTGACTTTAATATCCTG  
|-
|-
|
[[:Category:Kohara_Phage:2F5|2F5]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=162 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:21A9S|21A9S]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=163 Genobase]
|
PMID:3038334
|-  
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[[purK:Gene|purK79::Tn10]]
[[purK:Gene|purK79::Tn10]]
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CAG12171 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39380 CGSC7342]<ref name='LIB:cgsc'/>
CAG12171 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39380 CGSC7342]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:purE79::Tn10  
Synonyms:purE79::Tn10  
|-
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|
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[[dsbG:Gene|dsbG601::Tn10]]
[[dsbG:Gene|dsbG601::Tn10]]
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CAG12149 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39387 CGSC7346]<ref name='LIB:cgsc'/>
CAG12149 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39387 CGSC7346]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 19% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 19% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zbd-601::Tn10, zbe-601::Tn10


Synonyms:zbd-601::Tn10, zbe-601::Tn10
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6321 EcoCyc:G6321] 
EcoCyc:G6321
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3985 EchoBASE:EB3985] 
EcoGene:EG14234
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0573 EcoliGenExpDB:b0573] 
RegulonDB:ECK120002929
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C0659 ECOL199310CYC:C0659]
[http://www.ncbi.nlm.nih.gov/gene?term=945188 GeneID:945188]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
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|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG14234 EcoGene:EG14234] 
EchoBASE:EB3985
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=YLCC ECOO157CYC:YLCC] 
ASAP:ABE-0001967
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
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===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:cus genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:32, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cusF

Mnemonic

Cu sensor

Synonyms

ECK0565, b0573, JW0562, cusX, ylcC[1], silF, ylcC


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

12.85 minutes 

MG1655: 596354..596686
<gbrowseImage> name=NC_000913:596354..596686 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 578034..578366
<gbrowseImage> name=NC_012967:578034..578366 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 499114..499446
<gbrowseImage> name=NC_012759:499114..499446 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 596354..596686
<gbrowseImage> name=NC_007779:596354..596686 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 535686..536018
<gbrowseImage> name=NC_010473:535686..536018 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 648946..649278
<gbrowseImage> name=NC_010473:648946..649278 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

596417

Edman degradation

PMID:12813074[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

cusFM71I

M71I

Loss of copper resistance and strong decrease in copper binding; when associated with I-69

seeded from UniProt:P77214

cusFF73Y

F73Y

No change in copper resistance

seeded from UniProt:P77214

cusFHH57QQ

HH57QQ

Slight decrease in copper resistance

seeded from UniProt:P77214

cusFM69I

M69I

Loss of copper resistance and strong decrease in copper binding; when associated with I-71

seeded from UniProt:P77214

cusFHHH25QQQ

HHH25QQQ

No change in copper resistance

seeded from UniProt:P77214

cusFD48N

D48N

No change in copper resistance

seeded from UniProt:P77214

ΔcusF (Keio:JW0562)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8687[4]

ΔcusF782::kan

PMID:16738554[3]

CGSC:100444



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0562

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAAAAGCACTGCAAGTCGC

Primer 2:CCCTGGCTGACTTTAATATCCTG

2F5

Kohara Phage

Genobase

PMID:3038334[6]

21A9S

Kohara Phage

Genobase

PMID:3038334[6]

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[4]

est. P1 cotransduction: 14% [7]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[4]

est. P1 cotransduction: 19% [7]
Synonyms:zbd-601::Tn10, zbe-601::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6321

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14234

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002929

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945188

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3985

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001967

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Franke, S et al. (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J. Bacteriol. 185 3804-12 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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