araF:Gene: Difference between revisions
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<noinclude>{{TabsTop}} | <noinclude>{{TabsTop}} | ||
{{Tab|Quickview|araF}}{{Tab On|Gene|araF}}{{Tab|Gene Product(s)|araF}}{{Tab|Expression|araF}}{{Tab|Evolution|araF}}{{Tab|On One Page|araF}}{{TabsBottom}} | {{Tab|Quickview|araF}}{{Tab On|Gene|araF}}{{Tab|Gene Product(s)|araF}}{{Tab|Expression|araF}}{{Tab|Evolution|araF}}{{Tab|On One Page|araF}}{{TabsBottom}} | ||
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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</noinclude><protect> | </noinclude><protect> | ||
==Nomenclature== | ==Nomenclature== | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.. | {{BacktoTop}} | ||
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{| id="P46a9d191204a1" class=" tableEdit Gene_nomenclature_table" | |||
|- | |- | ||
!{{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
| | || | ||
''araF'' | ''araF'' | ||
|- | |||
!align=left {{table_heading_style}} |Mnemonic | |||
|| | |||
Arabinose | |||
|- | |- | ||
!{{table_heading_style}} | | !align=left {{table_heading_style}} |Synonyms | ||
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''ECK1899, b1901, JW1889''<ref name='LIB:Riley_2006'/>, JW1889'' | |||
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===Notes=== | ===Notes=== | ||
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==Location(s) and DNA Sequence== | ==Location(s) and DNA Sequence== | ||
{{BacktoTop}} | |||
<protect> | <protect> | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9d1919c74d" class=" tableEdit Gene_location_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
|- | |- | ||
| | | | ||
MG1655 | MG1655 | ||
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42.74 minutes | 42.74 minutes | ||
| | | | ||
MG1655: 1984152..1983163<br /> | MG1655: 1984152..1983163<br /><gbrowseImage> | ||
name=NC_000913:1983163..1984152 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10057 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10057 EcoCyc] | ||
{{GBrowseLink|MG1655|NC_000913|1982163|1985152|GBrowse(MG1655)}} | |||
| | | | ||
{{GBrowseFastaLink|MG1655|NC_000913|1983163|1984152|FASTA Dump}} | |||
*[http://biocyc.org/ECOLI/sequence?object=EG10057 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10057 EcoCyc Sequence Dump] | ||
|- | |- | ||
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[[REL606]] | |||
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NC_012967: 1964122..1963133<br /><gbrowseImage> | |||
name=NC_012967:1963133..1964122 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|1963133|1964122|GBrowse (REL606)}} | |||
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{{GBrowseFastaLink|REL606|NC_012967|1963133|1964122|FASTA Dump}} | |||
|- | |||
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[[BW2952]] | |||
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NC_012759: 1875646..1876635<br /><gbrowseImage> | |||
name=NC_012759:1875646..1876635 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|1875646|1876635|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|1875646|1876635|FASTA Dump}} | |||
|- | |||
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W3110 | W3110 | ||
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W3110: 1988265..1987276<br />{{ | W3110: 1988265..1987276<br /><gbrowseImage> | ||
name=NC_007779:1987276..1988265 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|W3110|NC_007779|1986276|1989265|GBrowse(W3110)}} | |||
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{{GBrowseFastaLink|W3110|NC_007779|1987276|1988265|FASTA Dump}} | |||
|- | |||
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[[DH10B]] | |||
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DH10B: 2075160..2074171<br /><gbrowseImage> | |||
name=NC_010473:2074171..2075160 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|DH10B|NC_010473|2072193|2077138|GBrowse(DH10B)}} | |||
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{{GBrowseFastaLink|DH10B|NC_010473|2072193|2077138|FASTA Dump}} | |||
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<!--Location_notes--></protect> | <!--Location_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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==Sequence Features== | ==Sequence Features== | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46a9d1925050f" class=" tableEdit Gene_sequence_features_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|-class= | |- | ||
|[{{SERVER}} | | | ||
Coding Start (SO:0000323) | |||
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[[MG1655]] | |||
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1983166 | |||
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Edman degradation | |||
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PMID:326784<br />PMID:9298646<br /> | |||
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<protect><!--Sequence_notes--></protect> | <protect><!--Sequence_notes--></protect> | ||
====Notes==== | ====Notes==== | ||
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==Alleles and Phenotypes== | ==Alleles and Phenotypes== | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46a9d1929439f" class=" tableEdit Gene_allele_table dataTable" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!CGSC | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|-class= | |- | ||
|[{{SERVER}} | | | ||
araFP277G | |||
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P277G | |||
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Improves binding to galactose | |||
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seeded from [http://www.uniprot.org/uniprot/P02924 UniProt:P02924] | |||
|- | |||
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ΔaraF (Keio:JW1889) | |||
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deletion | |||
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deletion | |||
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PMID:16738554 | |||
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107852 CGSC9571]<ref name='LIB:cgsc'/> | |||
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|- | |||
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0006330 araF::Tn5KAN-2] (FB20510) | |||
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Insertion at nt 512 in Minus orientation | |||
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PMID:15262929 | |||
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[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20510] | |||
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contains [[pKD46]] | |||
|- | |||
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0006330 araF::Tn5KAN-2] (FB20511) | |||
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Insertion at nt 512 in Minus orientation | |||
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PMID:15262929 | |||
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[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20511] | |||
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does not contain [[pKD46]] | |||
|- | |||
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''araF432'' | |||
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[[cgscmutation:80485|CGSC:80485]] | |||
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''araF434'' | |||
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[[cgscmutation:80488|CGSC:80488]] | |||
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''ΔaraF751::kan'' | |||
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PMID:16738554 | |||
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[[cgscmutation:100026|CGSC:100026]] | |||
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<protect><!--Alleles_notes--></protect> | <protect><!--Alleles_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.14373.C4e04b770da021--> | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C4e04b770da021" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.. | {{BacktoTop}} | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46a9d1934792b" class=" tableEdit Gene_resource_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
|- | |- | ||
|[{{SERVER}} | | | ||
JW1889 | |||
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Plasmid clone | |||
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | |||
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PMID:16769691 | |||
Status:Clone OK | |||
Primer 1:GCCCACAAATTTACTAAAGCCCT | |||
Primer 2:CCtTTACCGCCTAAACCTTTTTT | |||
|- | |||
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[[:Category:Kohara_Phage:7F11|7F11]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=339 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[:Category:Kohara_Phage:18A9|18A9]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=340 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[:Category:Kohara_Phage:16B12|16B12]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=341 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[eda:Gene|eda-51::Tn10]] | |||
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Linked marker | |||
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CAG18486 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39983 CGSC7392]<ref name='LIB:cgsc'/> | |||
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est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/> | |||
|- | |||
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[[uvrC:Gene|uvrC279::Tn10]] | |||
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Linked marker | |||
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CAG12156 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39987 CGSC7394]<ref name='LIB:cgsc'/> | |||
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est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> | |||
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<protect><!--Gene_resources_notes--></protect> | <protect><!--Gene_resources_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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==Accessions in Other Databases== | ==Accessions in Other Databases== | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.. | {{BacktoTop}} | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V46a9d193d97d0" class=" tableEdit Gene_accessions_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[[EcoCyc]] | |||
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EcoCyc:EG10057 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene:EG10057 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000053 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=946409 GeneID:946409] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0055 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[ASAP]] | |||
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ASAP:ABE-0006330 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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===Notes=== | ===Notes=== | ||
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==Links== | ==Links== | ||
{{BacktoTop}} | |||
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==References== | ==References== | ||
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__NOTOC__ | __NOTOC__ | ||
===Categories=== | |||
[[Category:Gene List:MG1655]] | |||
[[Category:Gene List:W3110]] | |||
[[Category:Genes in the Keio knockout collection]] | |||
[[Category:Genes in the ASKA clone collection]] | |||
[[Category:ara genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | |||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:39, 19 September 2013
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
araF |
|---|---|
| Mnemonic |
Arabinose |
| Synonyms |
ECK1899, b1901, JW1889[1], JW1889 |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
42.74 minutes |
MG1655: 1984152..1983163 |
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NC_012967: 1964122..1963133 |
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NC_012759: 1875646..1876635 |
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W3110 |
|
W3110: 1988265..1987276 |
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|
DH10B: 2075160..2074171 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1983166 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
araFP277G |
P277G |
Improves binding to galactose |
seeded from UniProt:P02924 | ||||
|
ΔaraF (Keio:JW1889) |
deletion |
deletion |
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|
araF::Tn5KAN-2 (FB20510) |
Insertion at nt 512 in Minus orientation |
contains pKD46 | |||||
|
araF::Tn5KAN-2 (FB20511) |
Insertion at nt 512 in Minus orientation |
does not contain pKD46 | |||||
|
araF432 |
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|
araF434 |
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|
ΔaraF751::kan |
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| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1889 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCACAAATTTACTAAAGCCCT Primer 2:CCtTTACCGCCTAAACCTTTTTT | |
|
Kohara Phage |
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|
Kohara Phage |
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|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 8% [9] | ||
|
Linked marker |
est. P1 cotransduction: 78% [9] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Hogg, RW & Hermodson, MA (1977) Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r. J. Biol. Chem. 252 5135-41 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).