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W3110: 4277461..4274639<br /><gbrowseImage>
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Sensitivity to
Sensitivity to
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Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.  
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions.
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PMID:17040104
PMID:17040104
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Strain:
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See Figure 1.
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Sensitivity to
Sensitivity to
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Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA.  Figure 7.
Mutation increases sensitivity to UV-radiation by causing the loss of uvrA's binding specificity between damaged and undamaged DNA.  Figure 7.
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PMID:8444906
PMID:8444906
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Parent Strain: MH1-∆A  
Parent Strain: MH1-∆A  
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''uvrA'' G275A
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Glycine to Alanine
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Resistant to
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Mutation creates "hyper" UV resistance greater than the UV resistance of wild type, figure 4.
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PMID:8444906
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Figure 4
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''uvrA'' C763F
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Cysteine to Phenylalanine
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Change in DNA binding
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Mutation causes loss of single-stranded DNA-binding activity.
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PMID:8444906
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No data shown
Parent Strain: MH1-A(del)
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''uvrA'' C740F H754Y C742Y/K750I
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Cysteine to Phenylalanine & Histidine to Tyrosine & Cysteine to Tyrosine/Lysine to Isoleucine (double mutant)
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Sensitivity to
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Mutation increased UV sensitivity by making the mutant proteins excision repair defective
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PMID:8444906
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No data shown in source.
Parent Strain: MH1-A(del)


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG11061
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
EcoGene:EG11061
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
RegulonDB:ECK120001050
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB1054
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0013290
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:51, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uvrA

Mnemonic

Ultraviolet resistant

Synonyms

ECK4050, b4058, JW4019, dar, dinE[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

92.01 minutes, 92.01 minutes 

MG1655: 4271894..4269072
<gbrowseImage> name=NC_000913:4269072..4271894 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4252803..4249981
<gbrowseImage> name=NC_012967:4249981..4252803 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4207917..4210739
<gbrowseImage> name=NC_012759:4207917..4210739 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4277461..4274639
<gbrowseImage> name=NC_007779:4274639..4277461 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4269072

Edman degradation

PMID:3007478[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

V332L

Nonsynonomous mutation

PMID:18245285[3]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

uvrA(del) (Keio:JW4019)

deletion

deletion

PMID:16738554[4]

Shigen

CGSC10889[5]

uvrAC253A,H,S

C253A,H,S

Reduced activity

seeded from UniProt:P0A698

uvrA6

Sensitivity to

Mutation increases sensitivity to UV-radiation

PMID:5335128[6]

CGSC:4736

Figure 5

Strain AB1157

uvrA157

CGSC:4765

uvrA103

CGSC:7745

uvrA155

CGSC:8494

uvrA277::Tn10

CGSC:8866

uvrA37

Sensitivity to

Mutation increases sensitivity to UV-radiation

PMID:5335128[6]

CGSC:9254

Figure 5

Strain Hfr J2

uvrA14

CGSC:9283

uvrA18

CGSC:9291

uvrA19

CGSC:9293

uvrA281

CGSC:10632

uvrA154

CGSC:11960

uvrA0

CGSC:17383

uvrA215::Mud(Ap,lac)

PMID:6771759[7] PMID:6780917[8]

CGSC:67398

uvrA753(del)::kan

PMID:16738554[4]

CGSC:103342

uvrA(del)::Tn10

deletion

deletion

Sensitivity to

Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.

PMID:17040104[9]

Experimental strain:GY5564

uvrA::Tn10 uvrC34

Sensitivity to

Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions.

PMID:17040104[9]

Strain:

See Figure 1.

uvrA(del)

deletion

deletion

Sensitivity to

Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.

PMID:16970815[10]

figure 2

uvrA K689A

Lysine to Alanine

change in binding affinity

Mutation caused a decrease in binding affinity to DNA, table 1.

PMID:18248777[11]

uvrA K680E

Lysine to Glutamic Acid

Change in binding affinity

Mutation caused a decrease in binding affinity to DNA, table 1.

PMID:18248777[11]

uvrA R691A

Arginine to Alanine

Change in DNA binding affinity

Mutation caused a decrease in binding affinity to DNA, table 1.

PMID:18248777[11]

uvrA R691D

Arginine to Aspartic Acid

Change in binding affinity

Mutation caused a decrease in binding affinity to DNA, table 1.

PMID:18248777[11]

uvrA K680A R691A

Lysine to Alanine & Arginine to Alanine

Change in binding affinity

Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.

PMID:18248777[11]

uvrA::Tn10 uvrC34

DNA repair

Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.

PMID:17040104[9]

Figure 4

uvrAB K45A

Lysine to alanine

change in enzyme activity

ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.

PMID:17630776[12]

ZnG uvrA

11 amino acid change, figure 1.

DNA binding

Mutant shows decreased binding affinity to damaged DNA, table 1.

PMID:16829526[13]

uvrA G502D V508D

Glycine to Aspartic Acid & Valine to Aspartic Acid

Sensitivity to

Mutation increases sensitivity to UV-radiation by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.

PMID:8444906[14]

Figure 7

Parent Strain: MH1-∆A

uvrA G275A

Glycine to Alanine

Resistant to

Mutation creates "hyper" UV resistance greater than the UV resistance of wild type, figure 4.

PMID:8444906[14]

Figure 4

uvrA C763F

Cysteine to Phenylalanine

Change in DNA binding

Mutation causes loss of single-stranded DNA-binding activity.

PMID:8444906[14]

No data shown

Parent Strain: MH1-A(del)

uvrA C740F H754Y C742Y/K750I

Cysteine to Phenylalanine & Histidine to Tyrosine & Cysteine to Tyrosine/Lysine to Isoleucine (double mutant)

Sensitivity to

Mutation increased UV sensitivity by making the mutant proteins excision repair defective

PMID:8444906[14]

No data shown in source.

Parent Strain: MH1-A(del)

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4019

Plasmid clone

Shigen

PMID:16769691[15]

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG

12D1

Kohara Phage

Genobase

PMID:3038334[16]

3F12

Kohara Phage

Genobase

PMID:3038334[16]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[5]

est. P1 cotransduction: 33% [17]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[5]

est. P1 cotransduction: 22% [17]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11061

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11061

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001050

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948559

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1054

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013290

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Husain, I et al. (1986) Sequences of Escherichia coli uvrA gene and protein reveal two potential ATP binding sites. J. Biol. Chem. 261 4895-901 PubMed
  3. ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 Howard-Flanders, P et al. (1966) Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53 1119-36 PubMed
  7. ↑ Kenyon, CJ & Walker, GC (1980) DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 77 2819-23 PubMed
  8. ↑ Kenyon, CJ & Walker, GC (1981) Expression of the E. coli uvrA gene is inducible. Nature 289 808-10 PubMed
  9. ↑ 9.0 9.1 9.2 Vidal, LS et al. (2006) Enhanced sensitivity of Escherichia coli uvrB mutants to mitomycin C points to a UV-C distinct repair for DNA adducts. Chem. Res. Toxicol. 19 1351-6 PubMed
  10. ↑ Crowley, DJ et al. (2006) The uvrA, uvrB and uvrC genes are required for repair of ultraviolet light induced DNA photoproducts in Halobacterium sp. NRC-1. Saline Syst. 2 11 PubMed
  11. ↑ 11.0 11.1 11.2 11.3 11.4 Croteau, DL et al. (2008) Cooperative damage recognition by UvrA and UvrB: identification of UvrA residues that mediate DNA binding. DNA Repair (Amst.) 7 392-404 PubMed
  12. ↑ Malta, E et al. (2007) Dynamics of the UvrABC nucleotide excision repair proteins analyzed by fluorescence resonance energy transfer. Biochemistry 46 9080-8 PubMed
  13. ↑ Croteau, DL et al. (2006) The C-terminal zinc finger of UvrA does not bind DNA directly but regulates damage-specific DNA binding. J. Biol. Chem. 281 26370-81 PubMed
  14. ↑ 14.0 14.1 14.2 14.3 Wang, J & Grossman, L (1993) Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA. J. Biol. Chem. 268 5323-31 PubMed
  15. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  16. ↑ 16.0 16.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  17. ↑ 17.0 17.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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