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W3110: 4248665..4247121<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|4246121|4249665|GBrowse(W3110)}}  
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Resistant to
Resistant to
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Thio(5)maltose resistance.
Thio(5)maltose increase resistance.
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PMID:6998971
PMID:6998971
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Selection for mutant maltose transport system


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG10555
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10555 ECOCYC:EG10555]
EcoGene:EG10555
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10555 EcoGene:EG10555]
RegulonDB:ECK120000548
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0550
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0013195
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:51, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malF

Mnemonic

Maltose

Synonyms

ECK4025, b4033, JW3993[1], JW3993, malJ

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.42 minutes, 91.42 minutes 

MG1655: 4243098..4241554
<gbrowseImage> name=NC_000913:4241554..4243098 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4224007..4222463
<gbrowseImage> name=NC_012967:4222463..4224007 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4180399..4181943
<gbrowseImage> name=NC_012759:4180399..4181943 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4248665..4247121
<gbrowseImage> name=NC_007779:4247121..4248665 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4342794..4341250
<gbrowseImage> name=NC_010473:4341250..4342794 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

malF(del) (Keio:JW3993)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC10874[3]

malFP420A

P420A

No effect

seeded from UniProt:P02916

malFG380C,S

G380C,S

No growth on maltose

seeded from UniProt:P02916

malFE401A,C,K,L

E401A,C,K,L

Reduction of transport rate

seeded from UniProt:P02916

malFS403C,D,K,L

S403C,D,K,L

Reduction of transport rate

seeded from UniProt:P02916

malFG407A,P

G407A,P

No effect

seeded from UniProt:P02916

malFL334W

L334W

Ability to transport lactose in a saturable manner

seeded from UniProt:P02916

malFL372W

L372W

Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose

seeded from UniProt:P02916

malFN376K,H

N376K,H

No growth on maltose

seeded from UniProt:P02916

malF3089::Tn10

Insertion at 4,242,342 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12164 = CGSC7476[3]

Synonyms:

malF64(Oc)

CGSC:7076

ochre (UAA) mutation

malF55::Tn10

CGSC:9952

malF59::Tn5

CGSC:9960

malF3089::Tn10

PMID:2540407[5]

CGSC:40369

malF3180::Tn10kan

PMID:2540407[5]

CGSC:40372

malF729(del)::kan

PMID:16738554[2]

CGSC:101732

malF(del)

deletion

deletion

Resistant to

Thio(5)maltose increase resistance.

PMID:6998971[6]

Selection for mutant maltose transport system

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3993

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGATGTCATTAAAAAGAAACA

Primer 2:CCATCAAACTTCATTCGCGTGGC

7A4

Kohara Phage

Genobase

PMID:3038334[8]

1F8

Kohara Phage

Genobase

PMID:3038334[8]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 12% [4]
Synonyms:thi-39::Tn10

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[3]

est. P1 cotransduction: 3% [4]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10555

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10555

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000548

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948532

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0550

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013195

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ 5.0 5.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  6. ↑ Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system. J. Bacteriol. 144 7-11 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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