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W3110: 3458938..3458393<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|3457393|3459938|GBrowse(W3110)}}  
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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nusG in [[MDS42]]
MDS42 ''nusG(del)''
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deletion
deletion
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fig. 2
see Figure 2A


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10667 ECOCYC:EG10667]
EcoCyc:EG10667
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:EG10667
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10667 EcoGene:EG10667]
RegulonDB:ECK120000660
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0661
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0013026
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:50, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nusG

Mnemonic

N (phage lambda protein) utilization substance

Synonyms

ECK3973, b3982, JW3945[1], JW3945

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90 minutes 

MG1655: 4175766..4176311
<gbrowseImage> name=NC_000913:4175766..4176311 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4157354..4157899
<gbrowseImage> name=NC_012967:4157354..4157899 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4065446..4065991
<gbrowseImage> name=NC_012759:4065446..4065991 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3458938..3458393
<gbrowseImage> name=NC_007779:3458393..3458938 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4275462..4276007
<gbrowseImage> name=NC_010473:4275462..4276007 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4175769

Edman degradation

PMID:9600841[2]
PMID:9740056[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

MDS42 nusG(del)

deletion

Sensitivity to

increases sensitivity to Bicyclomycin

PMID:21183718[4]

see Figure 2A

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3945

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCTGAAGCTCCTAAAAAGCG

Primer 2:CCGGCTTTTTCAACCTGGCTGAA

E11C11

Kohara Phage

Genobase

PMID:3038334[6]

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[7]

est. P1 cotransduction: 42% [8]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[7]

est. P1 cotransduction: 51% [8]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10667

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10667

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000660

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948485

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0661

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013026

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  3. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  4. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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