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W3110: 2660187..2658973<br /><gbrowseImage>
W3110: 2660187..2658973<br /><gbrowseImage>
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name=NC_007779:2658973..2660187
source=W3110
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{{GBrowseLink|W3110|AC_000091|2657973|2661187|GBrowse(W3110)}}  
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{{GBrowseFastaLink|W3110|AC_000091|2658973|2660187|FASTA Dump}}  
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a9e0388a635"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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''&#916;iscS776::kan''
''ΔiscS776::kan''
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deletion-replacement
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fig 2  
fig 2  
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iscS(del)::kan
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deletion-replacement
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Auxotrophies
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requires NAD for aerobic growth
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PMID:10908675
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NadA is inactive in the iscS(del) mutant because it contains an oxygen-sensitive [4Fe-4S] cluster that is required for activity.


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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:G7325
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7325 ECOCYC:G7325]
EcoGene:EG12677
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG12677 EcoGene:EG12677]
RegulonDB:ECK120003897
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB2542
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0008324
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:43, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

iscS

Mnemonic

Iron-sulfur cluster

Synonyms

ECK2527, b2530, JW2514, csdB, yfhO, nifS, yzzO, nuvC?[1][2], nuvC

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.3 minutes 

MG1655: 2659553..2658339
<gbrowseImage> name=NC_000913:2658339..2659553 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2582230..2581016
<gbrowseImage> name=NC_012967:2581016..2582230 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2544144..2545358
<gbrowseImage> name=NC_012759:2544144..2545358 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2660187..2658973
<gbrowseImage> name=NC_007779:2658973..2660187 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2751318..2750104
<gbrowseImage> name=NC_010473:2750104..2751318 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2658339

Edman degradation

PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔiscS (Keio:JW2514)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10002[5]

iscSC328A

C328A

Loss of cysteine desulfurization

seeded from UniProt:P0A6B7

ΔiscS776::kan

deletion-replacement

PMID:16738554[4]

CGSC:101588

iscS

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484[6]

fig 2

iscS(del)::kan

deletion-replacement

Auxotrophies

requires NAD for aerobic growth

PMID:10908675[7]

NadA is inactive in the iscS(del) mutant because it contains an oxygen-sensitive [4Fe-4S] cluster that is required for activity.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2514

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAATTACCGATTTATCTCGA

Primer 2:CCATGATGAGCCCATTCGATGCT

5E10

Kohara Phage

Genobase

PMID:3038334[9]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 3% [10]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 85% [10]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7325

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12677

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003897

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947004

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2542

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008324

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  7. ↑ Schwartz, CJ et al. (2000) The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97 9009-14 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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