poxB:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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* | * | ||
****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="N46a9ba93b1bb4" class=" tableEdit Gene_nomenclature_table" | ||
|- | |- | ||
!align=left {{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
|| | || | ||
''poxB'' | ''poxB'' | ||
|- | |- | ||
!align=left {{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
|| | || | ||
Pyruvate oxidase | |||
|- | |- | ||
!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK0862, b0871, JW0855''<ref name='LIB:Riley_2006'/> | ''ECK0862, b0871, JW0855''<ref name='LIB:Riley_2006'/>, JW0855'' | ||
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* | * | ||
****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9ba9435a7a" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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19.58 minutes | 19.58 minutes | ||
| | | | ||
MG1655: 910272..908554<br /> | MG1655: 910272..908554<br /><gbrowseImage> | ||
name=NC_000913:908554..910272 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10754 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10754 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|907554|911272|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|908554|910272|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10754 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10754 EcoCyc Sequence Dump] | ||
|- | |- | ||
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NC_012967: 927756..926038<br /> | NC_012967: 927756..926038<br /><gbrowseImage> | ||
name=NC_012967:926038..927756 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|REL606|NC_012967|926038|927756|GBrowse (REL606)}} | {{GBrowseLink|REL606|NC_012967|926038|927756|GBrowse (REL606)}} | ||
| | | | ||
{{GBrowseFastaLink|REL606|NC_012967|926038|927756|FASTA Dump}} | {{GBrowseFastaLink|REL606|NC_012967|926038|927756|FASTA Dump}} | ||
|- | |||
| | |||
[[BW2952]] | |||
| | |||
| | |||
NC_012759: 811522..813240<br /><gbrowseImage> | |||
name=NC_012759:811522..813240 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|811522|813240|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|811522|813240|FASTA Dump}} | |||
|- | |- | ||
| | | | ||
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| | ||
| | | | ||
W3110: 911471..909753<br /> | W3110: 911471..909753<br /><gbrowseImage> | ||
name=NC_007779:909753..911471 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|W3110|NC_007779|908753|912471|GBrowse(W3110)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|W3110|NC_007779|909753|911471|FASTA Dump}} | ||
|- | |- | ||
| | | | ||
| Line 110: | Line 143: | ||
| | | | ||
DH10B: 964200..962482<br /> | DH10B: 964200..962482<br /><gbrowseImage> | ||
name=NC_010473:962482..964200 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|959046|967636|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|959046|967636|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9ba94d35f8" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|- | |||
| | |||
Coding Start (SO:0000323) | |||
| | |||
[[MG1655]] | |||
| | |||
908554 | |||
| | |||
Edman degradation | |||
| | |||
PMID:3902830<br /> | |||
| | |||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9ba951f8b9" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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| | | | ||
Deletion | Deletion | ||
|- | |||
| | |||
poxBE564P | |||
| | |||
| | |||
E564P | |||
| | |||
| | |||
In poxB16; loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P07003 UniProt:P07003] | |||
|- | |||
| | |||
poxB | |||
| | |||
| | |||
| | |||
| | |||
| | |||
| | |||
| | |||
Deletion | |||
|- | |||
| | |||
poxBR572G | |||
| | |||
| | |||
R572G | |||
| | |||
| | |||
In poxB10; reduced activity; may interact less with membranes | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P07003 UniProt:P07003] | |||
|- | |- | ||
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|- | |- | ||
| | | | ||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | | | ||
| | |||
[[Biolog]]:respiration | |||
| | |||
unable to respire [[Carbon sources|b-Methyl-D-glucoside]] | |||
| | |||
PMID:16095938 | |||
| | | | ||
| | |||
|- | |||
| | |||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | | | ||
| | |||
[[Biolog]]:respiration | |||
| | |||
unable to respire [[Carbon sources|Acetate]] | |||
| | |||
PMID:16095938 | |||
| | | | ||
| | |||
|- | |||
| | |||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | | | ||
| | | | ||
[[ | [[Biolog]]:respiration | ||
| | |||
unable to respire [[Carbon sources|a-Hydroxybutyrate]] | |||
| | |||
PMID:16095938 | |||
| | |||
| | | | ||
|- | |- | ||
| | | | ||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | |||
| | |||
[[Biolog]]:respiration | |||
| | |||
unable to respire [[Carbon sources|a-Ketobutyrate]] | |||
| | |||
PMID:16095938 | |||
| | | | ||
| | |||
|- | |||
| | |||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | | | ||
| | |||
[[Biolog]]:respiration | |||
| | |||
unable to respire [[Carbon sources|a-Ketoglutarate]] | |||
| | |||
PMID:16095938 | |||
| | | | ||
| | |||
|- | |||
| | |||
ΔpoxB::kan | |||
| | |||
deletion | |||
| | | | ||
| | | | ||
PMID: | [[Biolog]]:respiration | ||
| | |||
unable to respire [[Carbon sources|Bromosuccinate]] | |||
| | |||
PMID:16095938 | |||
| | | | ||
| | | | ||
|- | |- | ||
| | | | ||
'' | ''poxB11'' | ||
| | | | ||
| Line 307: | Line 477: | ||
| | | | ||
| | | | ||
[[cgscmutation: | [[cgscmutation:10582|CGSC:10582]] | ||
| | | | ||
|- | |- | ||
| | | | ||
'' | ''poxB14::kan'' | ||
| | | | ||
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| | | | ||
PMID: | PMID:3045082 | ||
| | | | ||
[[cgscmutation: | [[cgscmutation:64804|CGSC:64804]] | ||
| | | | ||
|- | |- | ||
| | | | ||
''poxB15::lacZ::CmR'' | |||
| | |||
| | |||
| | |||
| | | | ||
| | | | ||
PMID:8022274 | |||
| | |||
[[cgscmutation:64823|CGSC:64823]] | |||
| | |||
|- | |||
| | |||
''ΔpoxB772::kan'' | |||
| | | | ||
| | | | ||
| | | | ||
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PMID:16738554 | |||
| | |||
[[cgscmutation:102414|CGSC:102414]] | |||
| | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R4e04c14e4c5d4" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.12301.R4e04c14e4c5d4&page=12301&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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****************************************************************************************** -->{{Gene_resources_help}} | ****************************************************************************************** -->{{Gene_resources_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9ba95bd503" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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Primer 2:CCCCTTAGCCAGTTTGTTTTCGC | Primer 2:CCCCTTAGCCAGTTTGTTTTCGC | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:14H6|14H6]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=211 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |- | ||
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****************************************************************************************** -->{{Gene_accessions_help}} | ****************************************************************************************** -->{{Gene_accessions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a9ba9652ce1" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[ | [[EcoCyc]] | ||
[ | |||
| | | | ||
EcoCyc:EG10754 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10754 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000745 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=946132 GeneID:946132] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0747 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0002958 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a6d7e7aae74" class=" tableEdit Links_table" | ||
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[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:pox genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | [[Category:pox genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | ||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:33, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
poxB |
|---|---|
| Mnemonic |
Pyruvate oxidase |
| Synonyms |
ECK0862, b0871, JW0855[1], JW0855 |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
19.58 minutes |
MG1655: 910272..908554 |
||
|
NC_012967: 927756..926038 |
||||
|
NC_012759: 811522..813240 |
||||
|
W3110 |
|
W3110: 911471..909753 |
||
|
DH10B: 964200..962482 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
908554 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpoxB (Keio:JW0855) |
deletion |
deletion |
|||||
|
poxBA553V |
A553V |
In poxB14 |
seeded from UniProt:P07003 | ||||
|
poxBD560P |
D560P |
In poxB15; normal activity |
seeded from UniProt:P07003 | ||||
|
poxB |
Deletion | ||||||
|
poxBE564P |
E564P |
In poxB16; loss of activity |
seeded from UniProt:P07003 | ||||
|
poxB |
Deletion | ||||||
|
poxBR572G |
R572G |
In poxB10; reduced activity; may interact less with membranes |
seeded from UniProt:P07003 | ||||
|
poxBA533T |
A533T |
In poxB11 |
seeded from UniProt:P07003 | ||||
|
poxB |
Deletion | ||||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
||||
|
poxB11 |
|||||||
|
poxB14::kan |
|||||||
|
poxB15::lacZ::CmR |
|||||||
|
ΔpoxB772::kan |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0855 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAACAAACGGTTGCAGCTTA Primer 2:CCCCTTAGCCAGTTTGTTTTCGC | |
|
Kohara Phage |
|||
|
zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: % [10] | |
|
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 21% [10] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Recny, MA et al. (1985) Characterization of the alpha-peptide released upon protease activation of pyruvate oxidase. J. Biol. Chem. 260 14287-91 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Chang, YY & Cronan, JE Jr (1988) Common ancestry of Escherichia coli pyruvate oxidase and the acetohydroxy acid synthases of the branched-chain amino acid biosynthetic pathway. J Bacteriol 170 3937-45 PubMed
- ↑ Chang, YY et al. (1994) Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol. Microbiol. 11 1019-28 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).