poxB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
poxB |
|---|---|
| Mnemonic |
Pyruvate oxidase |
| Synonyms |
ECK0862, b0871, JW0855[1], JW0855 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
19.58 minutes |
MG1655: 910272..908554 |
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NC_012967: 927756..926038 |
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NC_012759: 811522..813240 |
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W3110 |
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W3110: 911471..909753 |
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DH10B: 964200..962482 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
908554 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpoxB (Keio:JW0855) |
deletion |
deletion |
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poxBA553V |
A553V |
In poxB14 |
seeded from UniProt:P07003 | ||||
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poxBD560P |
D560P |
In poxB15; normal activity |
seeded from UniProt:P07003 | ||||
|
poxB |
Deletion | ||||||
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poxBE564P |
E564P |
In poxB16; loss of activity |
seeded from UniProt:P07003 | ||||
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poxB |
Deletion | ||||||
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poxBR572G |
R572G |
In poxB10; reduced activity; may interact less with membranes |
seeded from UniProt:P07003 | ||||
|
poxBA533T |
A533T |
In poxB11 |
seeded from UniProt:P07003 | ||||
|
poxB |
Deletion | ||||||
|
ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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ΔpoxB::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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poxB11 |
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poxB14::kan |
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poxB15::lacZ::CmR |
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ΔpoxB772::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0855 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAACAAACGGTTGCAGCTTA Primer 2:CCCCTTAGCCAGTTTGTTTTCGC | |
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Kohara Phage |
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zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: % [10] | |
|
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 21% [10] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Recny, MA et al. (1985) Characterization of the alpha-peptide released upon protease activation of pyruvate oxidase. J. Biol. Chem. 260 14287-91 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Chang, YY & Cronan, JE Jr (1988) Common ancestry of Escherichia coli pyruvate oxidase and the acetohydroxy acid synthases of the branched-chain amino acid biosynthetic pathway. J Bacteriol 170 3937-45 PubMed
- ↑ Chang, YY et al. (1994) Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol. Microbiol. 11 1019-28 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
