ftsK:Gene: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Convert to gbrowse_img.php extension.
touch
 
(21 intermediate revisions by 5 users not shown)
Line 16: Line 16:
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
Line 46: Line 47:
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK0881, b0890, JW0873, dinH''<ref name='LIB:Riley_2006'/>
''ECK0881, b0890, JW0873, dinH''<ref name='LIB:Riley_2006'/>, dinH'',
usoM <ref name='PMID:9294449'/>


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 67: Line 69:
*  
*  
****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|   id="V46a9bb01cfcb0"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9bb01cfcb0"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
Line 78: Line 80:
MG1655: 932447..936436<br /><gbrowseImage>
MG1655: 932447..936436<br /><gbrowseImage>
name=NC_000913:932447..936436
name=NC_000913:932447..936436
source=ecoli
source=MG1655
preset=GeneLocation
preset=GeneLocation
</gbrowseImage>
</gbrowseImage>
Line 84: Line 86:
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6464 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6464 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|931447|937436|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|931447|937436|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|932447|936436|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|932447|936436|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G6464 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G6464 EcoCyc Sequence Dump]  
|-  
|-  
Line 118: Line 120:


|
|
{{GBrowseLink|BW2952|012759|835415|NC_839404|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|835415|839404|GBrowse (BW2952)}}
|
|
{{GBrowseFastaLink|BW2952|012759|835415|NC_839404|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|835415|839404|FASTA Dump}}  
|-  
|-  
|
|
Line 128: Line 130:
|
|
W3110: 933646..937635<br /><gbrowseImage>
W3110: 933646..937635<br /><gbrowseImage>
name=AC_000091:933646..937635
name=NC_007779:933646..937635
source=ecoli_W3110
source=W3110
preset=GeneLocation
preset=GeneLocation
</gbrowseImage>
</gbrowseImage>


|
|
{{GBrowseLink|ecoli_W3110|AC_000091|932646|938635|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|932646|938635|GBrowse(W3110)}}  
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|933646|937635|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|933646|937635|FASTA Dump}}  
|-  
|-  
|
|
Line 145: Line 147:
DH10B: 986375..990364<br /><gbrowseImage>
DH10B: 986375..990364<br /><gbrowseImage>
name=NC_010473:986375..990364
name=NC_010473:986375..990364
source=ecoli_DH10B
source=DH10B
preset=GeneLocation
preset=GeneLocation
</gbrowseImage>
</gbrowseImage>


|
|
{{GBrowseLink|ecoli_DH10B|NC_010473|978397|998342|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|978397|998342|GBrowse(DH10B)}}
|
|
{{GBrowseFastaLink|ecoli_DH10B|NC_010473|978397|998342|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|978397|998342|FASTA Dump}}  


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 172: Line 174:
*  
*  
****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|   id="U46a9bb0279a81"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9bb0279a81"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
Line 194: Line 196:
*  
*  
****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{| {{Prettytable}} id="B46a9bb02b9f16"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9bb02b9f16"  class=" tableEdit Gene_allele_table dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
|-  
|
|
&Delta;LC
''LC(del)''
|
|


Line 256: Line 258:


|
|
 
|
|


Line 273: Line 275:


|
|
 
|
|


Line 290: Line 292:
K997A
K997A
|
|
 
|
|


Line 307: Line 309:


|
|
 
|
|


Line 339: Line 341:
''ftsk44''
''ftsk44''
|
|
 
missense mutation
|
|
G80A
G80A
Line 346: Line 348:
|
|
temperature sensitive late stage block in division, but does not affect chromosome replication or segregation
temperature sensitive late stage block in division, but does not affect chromosome replication or segregation
forms long filamentous cells at 42C
|
|
PMID:7592387
PMID:7592387
PMID:9721304
|
|
Donachie
Donachie
Line 360: Line 367:


|
|
Other
 
|
|
abnormal nucleoid segregation- asymmetrically positioned nucleoids and large anucleate regions
abnormal nucleoid segregation- asymmetrically positioned nucleoids and large anucleate regions
inhibited cell-cell separation
|
|
* PMID:9294448
* PMID:9294448
Line 372: Line 381:
|-  
|-  
|
|
''ftsK1::cat ΔmukB''
''ftsK1::cat mukB(del)''
|
|


Line 440: Line 449:
|-  
|-  
|
|
''ftsK::cat-Δ2 ΔdacA::kan''
''ftsK::cat-2(del) dacA(del)::kan''
|
|


Line 458: Line 467:
|-  
|-  
|
|
''ftsK::cat-Δ5 &Delta;dac::kan''
''ftsK::cat-5(del) dac(del)::kan''
|
|


Line 464: Line 473:


|
|
Other
 
|
|
synthetically lethal
synthetically lethal
Line 493: Line 502:
|-  
|-  
|
|
''ftsK1::cat ΔminB''
''ftsK1::cat minB(del)''
|
|


Line 521: Line 530:
|
|
PMID:18363794
PMID:18363794
|
|
|-
|
ftsK10
|
|
|
Growth Phenotype
|
affects expression form the uspA promoter, results in salt sensitivity, stationary-phase survival is impaired, and cell-cell separation is blocked
|
PMID:9294449
|
|
Also called usoM10
|-
|
ftsK
|
deletion in the c-terminal
|
|
Growth Phenotype
|
inhibits FtsK activity and increases recombination near dif
|
PMID:15916604
PMID:9294449
|
|


Line 534: Line 578:
===Notes===
===Notes===
Deletions that leave the membrane segments intact are viable<ref name='PMID: 9721304'/>.  Suppressors of the ''ftsK'' chaining/filamentation phenotype are discussed in Geissler and Margolin (2005)<ref name='PMID:16194242'/>.
Deletions that leave the membrane segments intact are viable<ref name='PMID: 9721304'/>.  Suppressors of the ''ftsK'' chaining/filamentation phenotype are discussed in Geissler and Margolin (2005)<ref name='PMID:16194242'/>.
==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12339.X4e04bb9e6c34f-->
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X4e04bb9e6c34f"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.12339.X4e04bb9e6c34f&page=12339&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
|}
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12339.X4e04bb9e6c34f--></protect>
<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
Line 544: Line 610:
*  
*  
****************************************************************************************** -->{{Gene_resources_help}}
****************************************************************************************** -->{{Gene_resources_help}}
{|   id="R46a9bb0364216"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R46a9bb0364216"  class=" tableEdit Gene_resource_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
Line 562: Line 628:


Primer 2:CCGTCAAACGGCGGTGGGGCCAG  
Primer 2:CCGTCAAACGGCGGTGGGGCCAG  
|-
|
[[:Category:Kohara_Phage:1F10|1F10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=214 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:E6H3|E6H3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=215 Genobase]
|
PMID:3038334
|-  
|-  
|
|
Line 718: Line 802:
|
|
PMID:18363794  
PMID:18363794  
|-
|
FtsKΔγ
|
Plasmid Clone
|
FtsK<sub>50C</sub> deleted from residue 1248
|
PMID:19854947


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 737: Line 830:
*  
*  
****************************************************************************************** -->{{Gene_accessions_help}}
****************************************************************************************** -->{{Gene_accessions_help}}
{|   id="G46a9bb03edc16"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46a9bb03edc16"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6464 EcoCyc:G6464]
EcoCyc:G6464
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3016 EchoBASE:EB3016]
EcoGene:EG13226
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0890 EcoliGenExpDB:b0890]
|
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C1027 ECOL199310CYC:C1027]
RegulonDB:ECK120003064
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG13226 EcoGene:EG13226]
[http://www.ncbi.nlm.nih.gov/gene?term=945102 GeneID:945102]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=FTSK ECOO157CYC:FTSK]
EchoBASE:EB3016
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003027&formSubmitReturn=1 ASAP:ABE-0003027]
ASAP:ABE-0003027
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
Line 808: Line 894:
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|   id="Y46a6d85840482"  class=" tableEdit Links_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Y46a6d85840482"  class=" tableEdit Links_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Name!!URL!!Comments
!|Name!!URL!!Comments

Latest revision as of 20:32, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ftsK

Mnemonic

filamentation temperature sensitive Filamentation, temperature sensitive

Synonyms

ECK0881, b0890, JW0873, dinH[1], dinH, usoM [2]

edit table


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

20.1 minutes 

MG1655: 932447..936436
<gbrowseImage> name=NC_000913:932447..936436 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 950282..954310
<gbrowseImage> name=NC_012967:950282..954310 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 835415..839404
<gbrowseImage> name=NC_012759:835415..839404 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 933646..937635
<gbrowseImage> name=NC_007779:933646..937635 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 986375..990364
<gbrowseImage> name=NC_010473:986375..990364 source=DH10B preset=GeneLocation </gbrowseImage>

edit table


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
edit table


Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

LC(del)

PMID:15522074[3]

Carries a deletion of FtsK L and FtsK C

ftsKG80A

G80A

In Toe44; loss of function under extreme conditions

seeded from UniProt:P46889

ftsKE58A

E58A

Loss of function

seeded from UniProt:P46889

NC

PMID:15522074[3]

Barre

Deletion of the FtsK Linker

NL hi C

PMID:15522074[3]

Barre

Carries swaps b/t E.coli and H. influenzae FtsK regions

ftsK A

K997A

PMID:15522074[3]

Barre

Carries a single point mutation in the walker A motif of FtsK C

NLC hi

PMID:15522074[3]

Barre

Carries swaps b/t E.coli and H.influenzae FtsK regions

ftsk::cat1

PMID:9721304[4]

Donachie Sheratt

only expresses the NTD due to a Cm resistance cassette at nt 678 of ftsK

ftsk44

missense mutation

G80A

Growth Phenotype

temperature sensitive late stage block in division, but does not affect chromosome replication or segregation

forms long filamentous cells at 42C

PMID:7592387[5]

PMID:9721304[4]

Donachie

this is the allele from the TOE44 strain listed in this table.

ftsK1::cat

abnormal nucleoid segregation- asymmetrically positioned nucleoids and large anucleate regions

inhibited cell-cell separation

defect in the C-terminus of FtsK

ftsK1::cat mukB(del)

Growth Phenotype

synthetically lethal

PMID:9829960[7]

shown that it must be complemented in trans

ftsK::cat-2

Δ54-2201

PMID:9721304[4]

almost the entire N-terminal membrane-spanning domain is deleted, and the C-terminal fragment is out of frame with the cat gene. Lethal- must be complemented in trans

ftsK::cat-Δ5

Δ54-3651

PMID:9721304[4]

this is a deletion of 90% of ftsK

ftsK3531

missense mutation

Growth Phenotype

same phenotype as ftsK44- temperature sensitive late stage block in division, but does not affect chromosome replication or segregation

PMID:9721304[4]

suppressed by high salt (1% NaCl) in the medium or by insertional inactivation of the dacA gene, encoding PBP5.

ftsK::cat-2(del) dacA(del)::kan

Cell Shape

  • forms small colonies on plates grows well
  • produces a population of misshapen cells in liquid culture

PMID:9721304[4]

ftsK::cat-5(del) dac(del)::kan

synthetically lethal

PMID:9721304[4]

ftsKC priA2::kan

Growth Phenotype

synthetically lethal

PMID:11459954[8]

ftsK1::cat minB(del)

Growth Phenotype

chains of minicells generated (many of which contained DNA)

PMID:9829960[7]

ftsK100

K997A

ATP mutant

PMID:18363794[9]

ftsK10

Growth Phenotype

affects expression form the uspA promoter, results in salt sensitivity, stationary-phase survival is impaired, and cell-cell separation is blocked

PMID:9294449[2]

Also called usoM10

ftsK

deletion in the c-terminal

Growth Phenotype

inhibits FtsK activity and increases recombination near dif

PMID:15916604[10] PMID:9294449[2]


edit table


Notes

Deletions that leave the membrane segments intact are viable[11]. Suppressors of the ftsK chaining/filamentation phenotype are discussed in Geissler and Margolin (2005)[12].


Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
edit table


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0873

Plasmid clone

Shigen

PMID:16769691[13]

Status:Clone OK

Primer 1:GCCAGCCAGGAATACATTGAAGA

Primer 2:CCGTCAAACGGCGGTGGGGCCAG

1F10

Kohara Phage

Genobase

PMID:3038334[14]

E6H3

Kohara Phage

Genobase

PMID:3038334[14]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[15]

est. P1 cotransduction: % [16]
Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[15]

est. P1 cotransduction: 61% [16]
Synonyms:zbj-1230::Tn10

W3110::Plac-ftsK

Strain

Depletion Strain[17]

pWL71

Plasmid Clone

Pbad-FtsK(1-202)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain[17]

pWL72

Plasmid Clone

Pbad-FtsK(1-211)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL73

Plasmid Clone

Pbad-FtsK(1-251)-GFP

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL74

Plasmid Clone

Pbad-FtsK(1-644)

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL75

Plasmid Clone

Pbad-FtsK(1-1329)

Successfully complements the W3110::Plac-ftsK depletion strain [17]

pWL76

Plasmid Clone

Pbad-FtsK(1-19 and 637-1329)- N-terminal 19 amino acids fused in phase to amino acid residue 637 of FtsK

Cannot complement the W3110::Plac-ftsK depletion strain [17]

pFtsK-PhoA plasmids

Plasmid Clone

This represents multiple plasmids with phoA inserted at various positions[18].

pFtsK-LacZ plasmids

Plasmid Clone

This represents multiple plasmids with lacZ inserted at various positions[18].

pFLAG-FtsK plasmids

Plasmid Clone

This reference describes plasmids with various FtsK truncations N-terminally tagged to FLAG[19].

MA3

Strain

AB1157ftsK

PMID:10523315[20]

GR51

Strain

AB1157xerCftsK

PMID:10523315[20]

GR59

Strain

AB1157ftsK recA56 srl::Tn10

PMID:10523315[20]

FC1

Strain

AB1157, ftsK100 K997A (ATP mutant), CmR

PMID:18363794[9]

FtsK-YFP

Plasmid Clone

  • low-copy expression vectors derived from pSC101
  • Spectinomycin resistance gene

PMID:18363794[9]

FtsKΔγ

Plasmid Clone

FtsK50C deleted from residue 1248

PMID:19854947[21]

edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6464

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13226

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003064

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945102

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3016

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003027

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 Arnau, J et al. (1997) Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase. J. Bacteriol. 179 5884-91 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 3.4 Bigot, S et al. (2004) FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol. Microbiol. 54 876-86 PubMed
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
  5. ↑ Begg, KJ et al. (1995) A new Escherichia coli cell division gene, ftsK. J. Bacteriol. 177 6211-22 PubMed
  6. ↑ Diez, AA et al. (1997) A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA. J. Bacteriol. 179 5878-83 PubMed
  7. ↑ 7.0 7.1 7.2 Yu, XC et al. (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol. 180 6424-8 PubMed
  8. ↑ McCool, JD & Sandler, SJ (2001) Effects of mutations involving cell division, recombination, and chromosome dimer resolution on a priA2::kan mutant. Proc. Natl. Acad. Sci. U.S.A. 98 8203-10 PubMed
  9. ↑ 9.0 9.1 9.2 Kennedy, SP et al. (2008) Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli. Mol. Microbiol. 68 1018-28 PubMed
  10. ↑ Corre, J & Louarn, JM (2005) Extent of the activity domain and possible roles of FtsK in the Escherichia coli chromosome terminus. Mol. Microbiol. 56 1539-48 PubMed
  11. ↑ Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
  12. ↑ Geissler, B & Margolin, W (2005) Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol. Microbiol. 58 596-612 PubMed
  13. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  14. ↑ 14.0 14.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  15. ↑ 15.0 15.1 CGSC: The Coli Genetics Stock Center
  16. ↑ 16.0 16.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  17. ↑ 17.0 17.1 17.2 17.3 17.4 17.5 17.6 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed
  18. ↑ 18.0 18.1 Dorazi, R & Dewar, SJ (2000) Membrane topology of the N-terminus of the Escherichia coli FtsK division protein. FEBS Lett. 478 13-8 PubMed
  19. ↑ Barre, FX et al. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14 2976-88 PubMed
  20. ↑ 20.0 20.1 20.2 Recchia, GD et al. (1999) FtsK-dependent and -independent pathways of Xer site-specific recombination. EMBO J. 18 5724-34 PubMed
  21. ↑ Graham, JE et al. (2010) FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 38 72-81 PubMed

Categories

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=ftsK:Gene&oldid=1476903"