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Product(s) |

Expression|

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Standard Name	ynjE
Gene Synonym(s)	ECK1755, b1757, JW5287^[1], JW5287
Product Desc.	predicted thiosulfate sulfur transferase^[2]^[3] Rhodanese-like protein, function unknown; possible lipoprotein, Cys conserved^[4]
Product Synonyms(s)	predicted thiosulfate sulfur transferase^[1], B1757^[2]^[1], YnjE^[2]^[1] , ECK1755, JW5287, b1757
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ynjE^[2]
Regulation/Activity
Quick Links
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Notes

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YnjE and SseA are paralogs; YnjE may be a second 3-mercaptopyruvate sulfurtransferase. Three rhodanese domains. Ambiguous signal prediction: First 17 aa are predicted to be a type II signal peptide; first 23 aa are predicted to be a type I signal peptide. YnjE is homologous to proteins with strong type II predictions. May reflect signal type II to I evolution.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ynjE
Mnemonic	Systematic nomenclature
Synonyms	ECK1755, b1757, JW5287^[1], JW5287
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	39.6 minutes	MG1655: 1837491..1838798 <gbrowseImage> name=NC_000913:1837491..1838798 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1816695..1818002 <gbrowseImage> name=NC_012967:1816695..1818002 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1729550..1730857 <gbrowseImage> name=NC_012759:1729550..1730857 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1841181..1842488 <gbrowseImage> name=NC_007779:1841181..1842488 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1928062..1929369 <gbrowseImage> name=NC_010473:1928062..1929369 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔynjE (Keio:JW5287)	deletion	deletion	PMID:16738554	Shigen CGSC11294^[5]
ΔynjE755::kan			PMID:16738554	CGSC:105844
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5287	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACGTGTTTCTCAAATGAC Primer 2:CCTTTGCTACTGTCCGGGCCGCG
4B8	Kohara Phage	Genobase	PMID:3038334
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 85% ^[6] Synonyms:zdi-276::Tn10
zea-225::Tn10	Linked marker	CAG18465 = CGSC7388^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:zdj-225::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6952	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14007	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003540	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946505	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3763	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005848	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YnjE
Synonyms	predicted thiosulfate sulfur transferase^[1], B1757^[2]^[1], YnjE^[2]^[1] , ECK1755, JW5287, b1757
Product description	predicted thiosulfate sulfur transferase^[2]^[3] Rhodanese-like protein, function unknown; possible lipoprotein, Cys conserved^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004792	thiosulfate sulfurtransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001307	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004792	thiosulfate sulfurtransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.8.1.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008272	sulfate transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001307	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016783	sulfurtransferase activity	PMID:19798741	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSGFSMKRVS QMTALAMALG LACASSWAAE LAKPLTLDQL QQQNGKAIDT RPSAFYNGWP QTLNGPSGHE LAALNLSASW LDKMSTEQLN AWIKQHNLKT DAPVALYGND KDVDAVKTRL QKAGLTHISI LSDALSEPSR LQKLPHFEQL VYPQWLHDLQ QGKEVTAKPA GDWKVIEAAW GAPKLYLISH IPGADYIDTN EVESEPLWNK VSDEQLKAML AKHGIRHDTT VILYGRDVYA AARVAQIMLY AGVKDVRLLD GGWQTWSDAG LPVERGTPPK VKAEPDFGVK IPAQPQLMLD MEQARGLLHR QDASLVSIRS WPEFIGTTSG YSYIKPKGEI AGARWGHAGS DSTHMEDFHN PDGTMRSADD ITAMWKAWNI KPEQQVSFYC GTGWRASETF MYARAMGWKN VSVYDGGWYE WSSDPKNPVA TGERGPDSSK
Length	440
Mol. Wt	48.738 kDa
pI	6.7 (calculated)
Extinction coefficient	119,860 - 120,110 (calc based on 14 Y, 18 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-23	UniProt Manual:Signal Peptides	UniProt:P78067
Domain	156..264	PF00581 Rhodanese-like domain		PMID:19920124
Domain	295..419	PF00581 Rhodanese-like domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ynjE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

A crystal structure was described by Hanzelman et al ^[7] in 2009.

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111324	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946505	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005848	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P78067	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6952	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14007	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946505	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003540	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3763	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.37E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	215	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	97	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	71	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ynjE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1837471..1837511 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1757 (EcoliWiki Page)	NCBI GEO profiles for ynjE
microarray	GenExpDB:b1757 (EcoliWiki Page)	Summary of data for ynjE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ynjE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6952	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3763	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1757
EcoGene	EcoGene:EG14007	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003540	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005848	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
E. coli O157	Z2789	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00581 Rhodanese-like domain
Pfam (EcoliWiki Page)	PF00581 Rhodanese-like domain
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Hänzelmann, P et al. (2009) Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains. Protein Sci. 18 2480-91 PubMed

ynjE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ynjE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Families

Notes

Links

References

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