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Standard Name	yjiA
Gene Synonym(s)	ECK4342, b4352, JW5790, yjiA'^[1]^[2]
Product Desc.	P-loop guanosine triphosphatase^[2]^[3] Putative GTPase, binds GTP, function unknown^[4]
Product Synonyms(s)	predicted GTPase ^[1], B4352 ^[2]^[1], YjiA ^[2]^[1] , ECK4342, JW5790, b4352
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yjiA^[2]
Regulation/Activity
Quick Links
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Notes

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Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yjiA
Mnemonic	Systematic nomenclature
Synonyms	ECK4342, b4352, JW5790, yjiA'^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	98.84 minutes, 98.84 minutes	MG1655: 4586888..4585932 <gbrowseImage> name=NC_000913:4585932..4586888 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4566840..4565884 <gbrowseImage> name=NC_012967:4565884..4566840 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4524416..4525372 <gbrowseImage> name=NC_012759:4524416..4525372 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4593545..4592589 <gbrowseImage> name=NC_007779:4592589..4593545 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔyjiA (Keio:JW5790)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5790	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACCCGATTGCAGTTACCCT Primer 2:CCtTTCCTCAACCCCGCAAACGC
10H12	Kohara Phage	Genobase	PMID:3038334
7C1	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: % ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: 78% ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10021	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10021	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000018	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948882	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0020	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014273	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YjiA
Synonyms	predicted GTPase ^[1], B4352 ^[2]^[1], YjiA ^[2]^[1] , ECK4342, JW5790, b4352
Product description	P-loop guanosine triphosphatase^[2]^[3] Putative GTPase, binds GTP, function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005525	GTP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0342	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	secA	PMID:19402753	LCMS(ID Probability):99.6
Protein	recA	PMID:19402753	LCMS(ID Probability):99.6
Protein	gsiB	PMID:19402753	LCMS(ID Probability):99.6
Protein	uspD	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplL	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIENEFGEVS VDDQLIGDRA TQIKTLTNGC ICCSRSNELE DALLDLLDNL DKGNIQFDRL VIECTGMADP GPIIQTFFSH EVLCQRYLLD GVIALVDAVH ADEQMNQFTI AQSQVGYADR ILLTKTDVAG EAEKLHERLA RINARAPVYT VTHGDIDLGL LFNTNGFMLE ENVVSTKPRF HFIADKQNDI SSIVVELDYP VDISEVSRVM ENLLLESADK LLRYKGMLWI DGEPNRLLFQ GVQRLYSADW DRPWGDEKPH STMVFIGIQL PEEEIRAAFA GLRK
Length	284
Mol. Wt	32.017 kDa
pI	4.4 (calculated)
Extinction coefficient	25,440 - 26,065 (calc based on 6 Y, 3 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	5..184	PF02492 CobW/HypB/UreG, nucleotide-binding domain	PMID:19920124
Domain	224..317	PF07683 Cobalamin synthesis protein cobW C-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yjiA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111738	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948882	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014273	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P24203	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10021	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10021	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948882	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000018	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0020	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.86E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	26.227+/-0.326	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.02039749	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	787	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	320	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	423	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yjiA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4586868..4586908 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4352 (EcoliWiki Page)	NCBI GEO profiles for yjiA
microarray	GenExpDB:b4352 (EcoliWiki Page)	Summary of data for yjiA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4586238..4586526) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ19; Well:E7^[7]
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Notes

Accessions Related to yjiA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10021	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0020	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4352
EcoGene	EcoGene:EG10021	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000018	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014273	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000033277 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G80480 (score: 1.000; bootstrap: 100%) AT1G15730 (score: 0.698) AT1G26520 (score: 0.117)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000000335 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000002857 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040426-2388 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-3335 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0204544 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000016477 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000348915 (score: 1.000; bootstrap: 100%) ENSP00000351418 (score: 0.987) ENSP00000353295 (score: 0.975) ENSP00000323433 (score: 0.972)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000021278 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000003838 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:2385089 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q69IK7 (score: 1.000; bootstrap: 100%) Q7XPT4 (score: 0.361) Q7XAM3 (score: 0.081)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000021209 (score: 1.000; bootstrap: 100%) ENSPTRP00000035891 (score: 0.088)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000021185 (score: 1.000; bootstrap: 100%) ENSRNOP00000000957 (score: 0.643) ENSRNOP00000034186 (score: 0.566)	From Inparanoid:20070104
Saccharomyces cerevisiae	YNR029C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000169738 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00032660001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000003488 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YJIA	From SHIGELLACYC
E. coli O157	Z5951	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02492 CobW/HypB/UreG, nucleotide-binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR13748:SF9
Superfamily (EcoliWiki Page)	SUPERFAMILY:90002
Pfam (EcoliWiki Page)	PF07683 Cobalamin synthesis protein cobW C-terminal domain
EcoCyc	EcoCyc:EG10021	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10021	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000018	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0020	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014273	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

yjiA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yjiA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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