yjhI:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yjhI

Gene Synonym(s)

ECK4288, b4299, JW4261[1], JW4261

Product Desc.

KpLE2 phage-like element; predicted DNA-binding transcriptional regulator[2][3]

Function unknown[4]

Product Synonyms(s)

KpLE2 phage-like element; predicted DNA-binding transcriptional regulator[1], B4299[2][1], YjhI[2][1] , ECK4288, JW4261, b4299

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yjhG[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yjhI

Mnemonic

Systematic nomenclature

Synonyms

ECK4288, b4299, JW4261[1], JW4261

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

97.49 minutes 

MG1655: 4523826..4523038
<gbrowseImage> name=NC_000913:4523038..4523826 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4461773..4462561
<gbrowseImage> name=NC_012759:4461773..4462561 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4530483..4529695
<gbrowseImage> name=NC_007779:4529695..4530483 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4625634..4624846
<gbrowseImage> name=NC_010473:4624846..4625634 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyjhI (Keio:JW4261)

deletion

deletion

PMID:16738554

Shigen
CGSC11054[5]

ΔyjhI765::kan

PMID:16738554

CGSC:105586


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4261

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGTTCGCAAAGGGTGTAATTC

Primer 2:CCGTTTCCTTTTCGTAAGTGCTC

9H9

Kohara Phage

Genobase

PMID:3038334

19H10

Kohara Phage

Genobase

PMID:3038334

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[5]

est. P1 cotransduction: % [6]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[5]

est. P1 cotransduction: 1% [6]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7912

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12551

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004407

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949100

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2439

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014092

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YjhI

Synonyms

KpLE2 phage-like element; predicted DNA-binding transcriptional regulator[1], B4299[2][1], YjhI[2][1] , ECK4288, JW4261, b4299

Product description

KpLE2 phage-like element; predicted DNA-binding transcriptional regulator[2][3]

Function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005471

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

PMID:15556475

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P16528

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005471

P

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

PMID:15556475

ISS: Inferred from Sequence or Structural Similarity

Swiss-Prot:P16528

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1147830

Protein

ydfK

PMID:16606699

Experiment(s):EBI-1147830

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1147830

Protein

groL

PMID:16606699

Experiment(s):EBI-1147830

Protein

ynaE

PMID:16606699

Experiment(s):EBI-1147830

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MVRKGCNSLV RAEKILTHIA WVGMASYMEL LNKFQYPKSS LLNLLNVMVD CGFLIKNKNG
YYSLGIKNYE LGCQALHRQN IFEVTKRPMQ ELSLKSGLVC HLGAMESISA IYLDKIESPD
SVPTSKSWIG KKLELHITAL GKALLAWKTR EELDYFLEAL TLTPHTRNTF TDKKLFLEEL
QKTRLRGWAI DNEESTYGAV CLSMPVFNMY NRVNYAISLS GDPVVYSGNK IDSYLELLRK
CAEQISYGLG YRNENEHLRK GN
Length

262

Mol. Wt

29.877 kDa

pI

8.9 (calculated)

Extinction coefficient

42,860 - 43,610 (calc based on 14 Y, 4 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

121..248

PF01614 Bacterial transcriptional regulator

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yjhI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16132120

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949100

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014092

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P39360

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7912

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12551

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949100

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004407

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2439

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

0.185+/-0.033

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.001269036

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

4a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yjhG

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4523806..4523846 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4299 (EcoliWiki Page)

NCBI GEO profiles for yjhI

microarray

GenExpDB:b4299 (EcoliWiki Page)

Summary of data for yjhI from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4523299..4523713) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ05; Well:G3[7]

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Notes

Accessions Related to yjhI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7912

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2439

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4299

EcoGene

EcoGene:EG12551

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004407

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014092

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000005256 (score: 1.000; bootstrap: 62%)

From Inparanoid:20070104


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01614 Bacterial transcriptional regulator

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:55781

EcoCyc

EcoCyc:G7912

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12551

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004407

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2439

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014092

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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