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Standard Name	yicO
Gene Synonym(s)	ECK3655, b3664, JW5636^[1], JW5636, pbuA
Product Desc.	putative membrane protein with possible relationship to novobiocin and deoxycholate resistance^[2]^[3] Probable adenine permease^[4]
Product Synonyms(s)	predicted xanthine/uracil permase^[1], B3664^[2]^[1], YicO^[2]^[1] , ECK3655, JW5636, pbuA, b3664
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yicO^[2]
Regulation/Activity
Quick Links
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Notes

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Paralogs YicO and PurP are 74% identical. PurP seems to account for all the high-affinity permease activity and it is unlikley that YicO is the low affinity activity, so YicO may need to be induced by BaeSR to get activity. yicO and the adajcent adenine deaminase gene ade may constitute an inducible adenine utilization divergon, however the convergent gene arrangement is not conserved outside of E. coli: the genes are adjacent in Klebsiella and Serratia in an apparent operon arrangement; this difference may reflect horizontal acquisition of ade, which is predicted to be an HT gene although yicO is a core gene, so their association may be a relatively recent evolutionary development. BaeSR regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yicO
Mnemonic	Systematic nomenclature
Synonyms	ECK3655, b3664, JW5636^[1], JW5636, pbuA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	82.77 minutes	MG1655: 3841812..3840478 <gbrowseImage> name=NC_000913:3840478..3841812 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3803721..3802387 <gbrowseImage> name=NC_012967:3802387..3803721 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3728811..3730145 <gbrowseImage> name=NC_012759:3728811..3730145 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3796626..3797960 <gbrowseImage> name=NC_007779:3796626..3797960 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3939396..3938062 <gbrowseImage> name=NC_010473:3938062..3939396 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔyicO (Keio:JW5636)	deletion	deletion	PMID:16738554	Shigen CGSC11493^[5]
ΔyicO769::kan			PMID:16738554	CGSC:105330
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5636	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAATGACAATACCGATTA Primer 2:CCATCCACCAGAATAATCTTCAG
1D11	Kohara Phage	Genobase	PMID:3038334
12D11	Kohara Phage	Genobase	PMID:3038334
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 64% ^[6]
zid-501::Tn10	Linked marker	CAG18499 = CGSC7458^[5]	est. P1 cotransduction: 26% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11691	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11691	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001634	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948174	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1642	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011975	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YicO
Synonyms	predicted xanthine/uracil permase^[1], B3664^[2]^[1], YicO^[2]^[1] , ECK3655, JW5636, pbuA, b3664
Product description	putative membrane protein with possible relationship to novobiocin and deoxycholate resistance^[2]^[3] Probable adenine permease^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006043	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006043	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006043	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006043	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized to the periplasm with 13 predicted transmembrane domains	Daley et al. (2005) ^[7]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIIITEPLLS FVLQKQGIKS PPMDKKMNND NTDYVSNESG TLSRLFKLPQ HGTTVRTELI AGMTTFLTMV YIVFVNPQIL GAAQMDPKVV FVTTCLIAGI GSIAMGIFAN LPVALAPAMG LNAFFAFVVV GAMGISWQTG MGAIFWGAVG LFLLTLFRIR YWMISNIPLS LRIGITSGIG LFIALMGLKN TGVIVANKDT LVMIGDLSSH GVLLGILGFF IITVLSSRHF HAAVLVSIVV TSCCGLFFGD VHFSGVYSIP PDISGVIGEV DLSGALTLEL AGIIFSFMLI NLFDSSGTLI GVTDKAGLID GNGKFPNMNK ALYVDSVSSV AGAFIGTSSV TAYIESTSGV AVGGRTGLTA VVVGVMFLLV MFFSPLVAIV PPYATAGALI FVGVLMTSSL ARVNWDDFTE SVPAFITTVM MPFTFSITEG IALGFMSYCI MKVCTGRWRD LNLCVVVVAA LFALKIILVD
Length	470
Mol. Wt	49.872 kDa
pI	6.6 (calculated)
Extinction coefficient	39,420 - 40,170 (calc based on 8 Y, 5 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	30..407	PF00860 Permease family		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yicO taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111631	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948174	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011975	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31440	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11691	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11691	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948174	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001634	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1642	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yicO
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3841792..3841832 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3664 (EcoliWiki Page)	NCBI GEO profiles for yicO
microarray	GenExpDB:b3664 (EcoliWiki Page)	Summary of data for yicO from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yicO Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11691	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1642	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3664
EcoGene	EcoGene:EG11691	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001634	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011975	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	YP_026186 (score: 0.095) AT3G10960 (score: 1.000; bootstrap: 100%) AT5G50300 (score: 0.353)	From Inparanoid:20070104
Oryza gramene	YP_026186 (score: 0.129) Q53MW1 (score: 1.000; bootstrap: 100%) Q5W735 (score: 0.144)	From Inparanoid:20070104
Schizosaccharomyces pombe	YP_026186 (score: 0.125) SPBC8877 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000019753 (score: 1.000; bootstrap: 100%) ENSXETP00000043294 (score: 0.084) ENSXETP00000041345 (score: 0.082) ENSXETP00000043295 (score: 0.056) YP_026186 (score: 0.836)	From Inparanoid:20070104
Shigella flexneri	YICO	From SHIGELLACYC
E. coli O157	Z5154	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00860 Permease family
EcoCyc	EcoCyc:EG11691	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11691	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001634	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1642	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011975	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

yicO:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yicO Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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