yibB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yibB

Gene Synonym(s)

ECK3608, b3618, JW5644, yibB, G7782, htrL[1][2], htrL

Product Desc.

involved in lipopolysaccharide biosynthesis[2][3]

RfaH-regulated high-temperature gene[4]

Product Synonyms(s)

predicted protein[1], B3618[2][1], YibB[2][1], YibB[2][1] , ECK3608, htrL, JW5644, b3618

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yibB[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yibB

Mnemonic

high temperature (requirement)

Synonyms

ECK3608, b3618, JW5644, yibB, G7782, htrL[1][2], htrL

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

81.71 minutes 

MG1655: 3791706..3790849
<gbrowseImage> name=NC_000913:3790849..3791706 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3679175..3680032
<gbrowseImage> name=NC_012759:3679175..3680032 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3846732..3847589
<gbrowseImage> name=NC_007779:3846732..3847589 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3889283..3888426
<gbrowseImage> name=NC_010473:3888426..3889283 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhtrL (Keio:JW5644)

deletion

deletion

PMID:16738554

Shigen
CGSC11496[5]

htrL::Tn5KAN-I-SceI (FB21319)

Insertion at nt 206 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21350

contains pKD46

ΔhtrL730::kan

PMID:16738554

CGSC:101438


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5644

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAATCATCCACCACAATTAT

Primer 2:CCTTTTCTTGAAAGGAAAATTCT

16B11

Kohara Phage

Genobase

PMID:3038334

5D2

Kohara Phage

Genobase

PMID:3038334

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[5]

est. P1 cotransduction: % [6]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[5]

est. P1 cotransduction: 22% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11188

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1174

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3618

EcoGene

EcoGene:EG11188

Escherichia coli str. K-12 substr. MG1655

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HTRL

ASAP

ASAP:ABE-0011838

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:EG11188

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948137

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001169

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YibB

Synonyms

predicted protein[1], B3618[2][1], YibB[2][1], YibB[2][1] , ECK3608, htrL, JW5644, b3618

Product description

involved in lipopolysaccharide biosynthesis[2][3]

RfaH-regulated high-temperature gene[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0009103

lipopolysaccharide biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0448

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MVLLVMKSST TIITAYFDIG RGDWTANKGF REKLARSVDV YFSYFERLAA LENEMIIFTS
PDLKPRVEAI RNGKPTTVIV IDIKKKFRYI RSRIEKIQKD ESFTNRLEPR QLKNPEYWSP
EYVLVCNLKA YFVNKAINMG LVKTPLVAWI DFGYCHKPNV TRGLKIWDFP FDESKMHLFT
IKKGLTVTSQ QQVFDFMIGN HVYIIGGAIV GSQHKWKEFY KLVLESQKIT LNNNIVDDDQ
GIFVMCYYKR PDLFNLNYLG RGKWFDLFRC FRSNTLGAKM QALRIFLSRK
Length

290

Mol. Wt

34.014 kDa

pI

10.0 (calculated)

Extinction coefficient

52,370 - 52,870 (calc based on 13 Y, 6 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..277

PF09612 Bacterial protein of unknown function (HtrL_YibB)

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=htrL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111622

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948137

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011838

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P25666

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11188

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11188

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948137

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001169

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1174

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

11.33+/-0.093

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.033838973

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

654

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

160

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

273

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

htrL

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3791686..3791726 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3618 (EcoliWiki Page)

NCBI GEO profiles for htrL

microarray

GenExpDB:b3618 (EcoliWiki Page)

Summary of data for htrL from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3791250..3791645) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ19; Well:B9[7]

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Notes

Accessions Related to yibB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11188

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1174

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3618

EcoGene

EcoGene:EG11188

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001169

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011838

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Caenorhabditis briggsae

  • WBGene00041616 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00011713 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

E. coli O157

HTRL

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF09612 Bacterial protein of unknown function (HtrL_YibB)


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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