yiaK:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yiaK

Gene Synonym(s)

ECK3564, b3575, JW3547[1], JW3547

Product Desc.

2,3-diketo-L-gulonate reductase[2][3];

Component of 2,3-diketo-L-gulonate reductase[3]

L-dehydroascorbate (2,3-diketo-L-gulonate) reductase, NADH-dependent[4]

Product Synonyms(s)

2,3-diketo-L-gulonate dehydrogenase, NADH-dependent[1], B3575[2][1], YiaK[2][1] , ECK3564, JW3547, b3575

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yiaKLMNO-lyxK-sgbHUE[2], yiaKLMNOPQRS

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yiaK

Mnemonic

Systematic nomenclature

Synonyms

ECK3564, b3575, JW3547[1], JW3547

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

80.63 minutes, 80.63 minutes 

MG1655: 3740756..3741754
<gbrowseImage> name=NC_000913:3740756..3741754 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3673189..3674187
<gbrowseImage> name=NC_012967:3673189..3674187 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3629082..3630080
<gbrowseImage> name=NC_012759:3629082..3630080 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3897682..3896684
<gbrowseImage> name=NC_007779:3896684..3897682 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3838333..3839331
<gbrowseImage> name=NC_010473:3838333..3839331 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyiaK (Keio:JW3547)

deletion

deletion

PMID:16738554

Shigen
CGSC10617[5]

yiaK::Tn5KAN-I-SceI (FB21304)

Insertion at nt 234 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21304

contains pKD46

yiaK::Tn5KAN-I-SceI (FB21305)

Insertion at nt 234 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21305

does not contain pKD46

ΔyiaK758::kan

PMID:16738554

CGSC:105284


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3547

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAGTGACATTTGAGCAGTT

Primer 2:CCTAACGCCTGGATTTTGGCCCA

9B3

Kohara Phage

Genobase

PMID:3038334

7C3

Kohara Phage

Genobase

PMID:3038334

10C8

Kohara Phage

Genobase

PMID:3038334

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[5]

est. P1 cotransduction: % [6]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[5]

est. P1 cotransduction: % [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12279

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12279

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002160

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948096

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2187

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011682

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YiaK

Synonyms

2,3-diketo-L-gulonate dehydrogenase, NADH-dependent[1], B3575[2][1], YiaK[2][1] , ECK3564, JW3547, b3575

Product description

2,3-diketo-L-gulonate reductase[2][3];

Component of 2,3-diketo-L-gulonate reductase[3]

L-dehydroascorbate (2,3-diketo-L-gulonate) reductase, NADH-dependent[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00820

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0047559

3-dehydro-L-gulonate 2-dehydrogenase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00820

F

Seeded from EcoCyc (v14.0)

complete

GO:0047559

3-dehydro-L-gulonate 2-dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.130

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003767

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of 2,3-diketo-L-gulonate reductase

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKVTFEQLKA AFNRVLISRG VDSETADACA EMFARTTESG VYSHGVNRFP RFIQQLENGD
IIPDAQPKRI TSLGAIEQWD AQRSIGNLTA KKMMDRAIEL AADHGIGLVA LRNANHWMRG
GSYGWQAAEK GYIGICWTNS IAVMPPWGAK ECRIGTNPLI VAIPSTPITM VDMSMSMFSY
GMLEVNRLAG RQLPVDGGFD DEGNLTKEPG VIEKNRRILP MGYWKGSGMS IVLDMIATLL
SDGASVAEVT QDNSDEYGIS QIFIAIEVDK LIDGPTRDAK LQRIMDYVTS AERADENQAI
RLPGHEFTTL LAENRRNGIT VDDSVWAKIQ AL
Length

332

Mol. Wt

36.573 kDa

pI

5.1 (calculated)

Extinction coefficient

48,930 - 49,305 (calc based on 7 Y, 7 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..332

PF02615 Malate/L-lactate dehydrogenase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yiaK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131446

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948096

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011682

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P37672

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12279

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12279

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948096

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002160

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2187

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

8a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yiaKLMNO-lyxK-sgbHUE

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3740736..3740776 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3575 (EcoliWiki Page)

NCBI GEO profiles for yiaK

microarray

GenExpDB:b3575 (EcoliWiki Page)

Summary of data for yiaK from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to yiaK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12279

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2187

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3575

EcoGene

EcoGene:EG12279

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002160

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011682

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

YIAK

From SHIGELLACYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02615 Malate/L-lactate dehydrogenase

Superfamily (EcoliWiki Page)

SUPERFAMILY:89733

EcoCyc

EcoCyc:EG12279

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12279

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002160

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2187

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011682

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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