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Expression|

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Standard Name	yhiN
Gene Synonym(s)	ECK3477, b3492, JW3459^[1], JW3459
Product Desc.	predicted oxidoreductase with FAD/NAD(P)-binding domain^[2]^[3] Function unknown^[4]
Product Synonyms(s)	predicted oxidoreductase with FAD/NAD(P)-binding domain^[1], B3492^[2]^[1], YhiN^[2]^[1] , ECK3477, JW3459, b3492
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yhiN^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yhiN
Mnemonic	Systematic nomenclature
Synonyms	ECK3477, b3492, JW3459^[1], JW3459
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	78.33 minutes	MG1655: 3635433..3634231 <gbrowseImage> name=NC_000913:3634231..3635433 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3568099..3566897 <gbrowseImage> name=NC_012967:3566897..3568099 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3522725..3523927 <gbrowseImage> name=NC_012759:3522725..3523927 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4003005..4004207 <gbrowseImage> name=NC_007779:4003005..4004207 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3733178..3731976 <gbrowseImage> name=NC_010473:3731976..3733178 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔyhiN (Keio:JW3459)	deletion	deletion	PMID:16738554	Shigen CGSC10562^[5]
yhiN::Tn5KAN-I-SceI (FB21236)	Insertion at nt 616 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21236	contains pKD46
yhiN::Tn5KAN-I-SceI (FB21237)	Insertion at nt 616 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21237	does not contain pKD46
ΔyhiN748::kan			PMID:16738554	CGSC:105244
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3459	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAAAGGTTTGATGCCATTAT Primer 2:CCGGACGACTTTGCTGCAATCAA
12E4	Kohara Phage	Genobase	PMID:3038334
6A4	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: 15% ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12229	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12229	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002113	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947996	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2141	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011402	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YhiN
Synonyms	predicted oxidoreductase with FAD/NAD(P)-binding domain^[1], B3492^[2]^[1], YhiN^[2]^[1] , ECK3477, JW3459, b3492
Product description	predicted oxidoreductase with FAD/NAD(P)-binding domain^[2]^[3] Function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplI	PMID:16606699	Experiment(s):EBI-1146016
Protein	nadE	PMID:16606699	Experiment(s):EBI-1146016
Protein	rplB	PMID:16606699	Experiment(s):EBI-1146016
Protein	groL	PMID:16606699	Experiment(s):EBI-1146016
Protein	glmM	PMID:16606699	Experiment(s):EBI-1146016
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1146016
Protein	recF	PMID:16606699	Experiment(s):EBI-1146016
Protein	hsdS	PMID:19402753	LCMS(ID Probability):99.4
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MERFDAIIIG AGAAGMFCSA LAGQAGRRVL LIDNGKKPGR KILMSGGGRC NFTNLYVEPG AYLSQNPHFC KSALARFTQW DFIDLVNKHG IAWHEKTLGQ LFCDDSAQQI VDMLVDECEK GNVTFRLRSE VLSVAKDETG FTLDLNGMTV GCEKLVIATG GLSMPGLGAS PFGYKIAEQF GLNVLPTRAG LVPFTLHKPL LEELQVLAGV AVPSVITAEN GTVFRENLLF THRGLSGPAV LQISSYWQPG EFVSINLLPD VDLETFLNEQ RNAHPNQSLK NTLAVHLPKR LVERLQQLGQ IPDVSLKQLN VRDQQALIST LTDWRVQPNG TEGYRTAEVT LGGVDTNELS SRTMEARKVP GLYFIGEVMD VTGWLGGYNF QWAWSSAWAC AQDLIAAKSS
Length	400
Mol. Wt	43.741 kDa
pI	6.2 (calculated)
Extinction coefficient	54,430 - 55,305 (calc based on 7 Y, 8 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..393	PF03486 HI0933-like protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yhiN taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131364	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947996	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011402	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37631	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12229	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12229	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947996	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002113	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2141	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.47E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	451	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	92	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	138	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yhiN
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3635413..3635453 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3492 (EcoliWiki Page)	NCBI GEO profiles for yhiN
microarray	GenExpDB:b3492 (EcoliWiki Page)	Summary of data for yhiN from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yhiN Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12229	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2141	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3492
EcoGene	EcoGene:EG12229	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002113	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011402	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G39940 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000013940 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YHIN	From SHIGELLACYC
E. coli O157	YHIN	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03486 HI0933-like protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:51905
Panther (EcoliWiki Page)	PTHR10668:SF10
EcoCyc	EcoCyc:EG12229	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12229	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002113	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2141	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011402	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yhiN:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yhiN Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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