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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	yfbS
Gene Synonym(s)	ECK2286, b2292, JW2289^[1], JW2289
Product Desc.	putative transport protein^[2]^[3] Putative transporter, function unknown^[4]
Product Synonyms(s)	predicted transporter^[1], B2292^[2]^[1], YfbS^[2]^[1] , ECK2286, JW2289, b2292
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yfbS^[2]
Regulation/Activity
Quick Links
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Notes

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IT (Ion Transporter) superfamily (Prakash, 2003). ST(3) structural class based on hydropathy profile (Lolkema, 2003).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yfbS
Mnemonic	Systematic nomenclature
Synonyms	ECK2286, b2292, JW2289^[1], JW2289
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	51.89 minutes	MG1655: 2409374..2407542 <gbrowseImage> name=NC_000913:2407542..2409374 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2354943..2353111 <gbrowseImage> name=NC_012967:2353111..2354943 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2293347..2295179 <gbrowseImage> name=NC_012759:2293347..2295179 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2416798..2414966 <gbrowseImage> name=NC_007779:2414966..2416798 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔyfbS (Keio:JW2289)	deletion	deletion			PMID:16738554	Shigen CGSC9841^[5]
ΔyfbS::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔyfbS774::kan					PMID:16738554	CGSC:104662
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2289	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACGGTGAATTGATTTGGGT Primer 2:CCAAACGGAAACAGCATCGGGAT
9C2	Kohara Phage	Genobase	PMID:3038334
E9C9	Kohara Phage	Genobase	PMID:3038334
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[5]	est. P1 cotransduction: 52% ^[6] Synonyms:zej-223::Tn10
zfd-1::Tn10	Linked marker	CAG18467 = CGSC7408^[5]	est. P1 cotransduction: % ^[6] Synonyms:zfb-1::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7186	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14103	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003761	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946766	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3856	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007565	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YfbS
Synonyms	predicted transporter^[1], B2292^[2]^[1], YfbS^[2]^[1] , ECK2286, JW2289, b2292
Product description	putative transport protein^[2]^[3] Putative transporter, function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001898	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005488	binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006037	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006814	sodium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001898	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008324	cation transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006037	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015137	citrate transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015746	citrate transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004680	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001898	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004680	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001898	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004680	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized to the periplasm with 10 predicted transmembrane domains	Daley et al. (2005) ^[7]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNGELIWVLS LLAVAIVLFA TGRVRMDAVA LFVIVAFALS GTLTVPEVFS GFSDPNVVLI AALFIIGDGL VRTGVATVMG TWLVKVAGNS EIKMLVLLML TVAGLGAFMS STGVVAIFIP VVLSVAMRMQ TSPSRLMMPL SFAGLISGMM TLVATPPNLV VNSELLREGY HGFSFFSVTP IGLVVLVLGI LYMLVMRFML KGDTQTPQRE GWTRRTFRDL IREYRLTGRA RRLAIRPGSP MIGQRLDDLK LRERYGANVI GVERWRRFRR VIVNVNGVSE FRARDVLLID MSAADVDLRQ FCSEQLLEPM VLRGEYFSDQ ALDVGMAEIS LIPESELIGK SVREIGFRTR YGLNVVGLKR NGVALEGSLA DEPLLLGDII LVVGNWKLIG MLAKQGRDFV ALNLPEEVSE ASPAHSQAPH AIFCLVLMVA LMLTDEIPNP VAAIIACLLM GKFRCIDAES AYKSIHWPSI ILIVGMMPFA VALQKTGGVA LAVKGLMDIG GGYGPHMMLG CLFVLSAVIG LFISNTATAV LMAPIALAAA KTMGVSPYPF AMVVAMAASA AFMTPVSSPV NTLVLGPGNY SFSDFVKLGV PFTIIVMAVC VVMIPMLFPF
Length	610
Mol. Wt	65.872 kDa
pI	8.8 (calculated)
Extinction coefficient	47,900 - 48,650 (calc based on 10 Y, 6 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	9..558	PF03600 Citrate transporter	PMID:19920124
Domain	230..305	PF02080 TrkA-C domain	PMID:19920124
Domain	326..395	PF02080 TrkA-C domain	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yfbS taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130227	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946766	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007565	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFU2	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7186	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14103	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946766	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003761	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3856	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	21	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	13	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	24^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yfbS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2409354..2409394 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2292 (EcoliWiki Page)	NCBI GEO profiles for yfbS
microarray	GenExpDB:b2292 (EcoliWiki Page)	Summary of data for yfbS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2409287..2409528) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:B9^[8]
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Notes

Accessions Related to yfbS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7186	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3856	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2292
EcoGene	EcoGene:EG14103	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003761	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007565	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G47560 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00031317 (score: 1.000; bootstrap: 100%) WBGene00029597 (score: 0.347) WBGene00037575 (score: 0.227) WBGene00029030 (score: 0.174)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00003519 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA20251-PA (score: 1.000; bootstrap: 100%) GA17201-PA (score: 0.146)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000007061 (score: 1.000; bootstrap: 100%) ENSGALP00000005772 (score: 0.343) ENSGALP00000009629 (score: 0.339) ENSGALP00000021021 (score: 0.276) ENSGALP00000014400 (score: 0.270)	From Inparanoid:20070104
Mus musculus	MGI:2442367 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ZFH7 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000038185 (score: 1.000; bootstrap: 100%) ENSRNOP00000020043 (score: 0.446)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC3B84c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000134070 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000183045 (score: 0.364)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000023252 (score: 1.000; bootstrap: 100%) ENSXETP00000049224 (score: 0.545) ENSXETP00000017909 (score: 0.473) ENSXETP00000042738 (score: 0.438) ENSXETP00000051278 (score: 0.424)	From Inparanoid:20070104
Shigella flexneri	YFBS	From SHIGELLACYC
E. coli O157	YFBS	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03600 Citrate transporter
Panther (EcoliWiki Page)	PTHR10283:SF12
Superfamily (EcoliWiki Page)	SUPERFAMILY:116726
Superfamily (EcoliWiki Page)	SUPERFAMILY:116726
Pfam (EcoliWiki Page)	PF02080 TrkA-C domain
Pfam (EcoliWiki Page)	PF02080 TrkA-C domain
EcoCyc	EcoCyc:G7186	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14103	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003761	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3856	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007565	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

yfbS:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yfbS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

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