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Expression|

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Standard Name	yeiP
Gene Synonym(s)	ECK2165, b2171, JW5362^[1], JW5362
Product Desc.	predicted dehydrogenase, NAD-dependent^[2]^[3] Function unknown, low abundance protein^[4]
Product Synonyms(s)	predicted elongtion factor^[1], B2171^[2]^[1], YeiP^[2]^[1] , ECK2165, JW5362, b2171
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yeiP^[2]
Regulation/Activity
Quick Links
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Notes

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yeiP is a non-essential gene.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yeiP
Mnemonic	Systematic nomenclature
Synonyms	ECK2165, b2171, JW5362^[1], JW5362
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	48.79 minutes	MG1655: 2263472..2264044 <gbrowseImage> name=NC_000913:2263472..2264044 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2216638..2217210 <gbrowseImage> name=NC_012967:2216638..2217210 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 2268784..2269356 <gbrowseImage> name=NC_007779:2268784..2269356 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2354460..2355032 <gbrowseImage> name=NC_010473:2354460..2355032 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔyeiP (Keio:JW5362)	deletion	deletion	PMID:16738554	Shigen CGSC11342^[5]
yeiP::Tn5KAN-I-SceI (FB20668)	Insertion at nt 504 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20668	contains pKD46
ΔyeiP728::kan			PMID:16738554	CGSC:104582
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5362	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCAAGAGCGAACGAAATTAA Primer 2:CCGTCAGCACGCCCCATATAACG
12C12	Kohara Phage	Genobase	PMID:3038334
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
napA722::Tn10	Linked marker	CAG12098 = CGSC7400^[5]	est. P1 cotransduction: 21% ^[6] Synonyms:zeg-722, zei-722::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001952	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946674	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1970	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007187	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YeiP
Synonyms	predicted elongtion factor^[1], B2171^[2]^[1], YeiP^[2]^[1] , ECK2165, JW5362, b2171
Product description	predicted dehydrogenase, NAD-dependent^[2]^[3] Function unknown, low abundance protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003746	translation elongation factor activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00646	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003746	translation elongation factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001059	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006414	translational elongation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00646	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006414	translational elongation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001059	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQGVLAGLPS GIPGIGLEIE GAVQHAPQPG RHSIRCSLKV NHRIDERAQY HLFCTSKSAN TTCPKGPSRV LCRDIFDIFD YRNFSMPRAN EIKKGMVLNY NGKLLLVKDI DIQSPTARGA ATLYKMRFSD VRTGLKVEER FKGDDIVDTV TLTRRYVDFS YVDGNEYVFM DKEDYTPYTF TKDQIEEELL FMPEGGMPDM QVLTWDGQLL ALELPQTVDL EIVETAPGIK GASASARNKP ATLSTGLVIQ VPEYLSPGEK IRIHIEERRY MGRAD
Length	275
Mol. Wt	30.895 kDa
pI	6.1 (calculated)
Extinction coefficient	21,890 - 22,390 (calc based on 11 Y, 1 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	69..125	PF01132 Elongation factor P (EF-P) OB domain	PMID:19920124
Domain	3..62	PF08207 Elongation factor P (EF-P) KOW-like domain	PMID:19920124
Domain	133..188	PF09285 Elongation factor P, C-terminal	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yeiP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111399	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946674	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007187	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6N8	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12035	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946674	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001952	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1970	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	113.635+/-0.578	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.068181818	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	8692	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1752	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	3751	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yeiP
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2263452..2263492 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2171 (EcoliWiki Page)	NCBI GEO profiles for yeiP
microarray	GenExpDB:b2171 (EcoliWiki Page)	Summary of data for yeiP from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2262993..2263269) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ05; Well:B10^[7]
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Notes

Accessions Related to yeiP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12035	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1970	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2171
EcoGene	EcoGene:EG12035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001952	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007187	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Dictyostelium discoideum	DDB0217192 (score: 1.000; bootstrap: 100%) DDB0168187 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YEIP	From SHIGELLACYC
E. coli O157	YEIP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01132 Elongation factor P (EF-P) OB domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:50104
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Pfam (EcoliWiki Page)	PF08207 Elongation factor P (EF-P) KOW-like domain
Pfam (EcoliWiki Page)	PF09285 Elongation factor P, C-terminal
EcoCyc	EcoCyc:EG12035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001952	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1970	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007187	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

yeiP:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yeiP Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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