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Gene |

Product(s) |

Expression|

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Standard Name	yegS
Gene Synonym(s)	ECK2082, b2086, JW2070^[1], JW2070
Product Desc.	conserved protein^[2]; lipid kinase^[3]
Product Synonyms(s)	conserved protein^[1], B2086^[2]^[1], YegS^[2]^[1] , ECK2082, JW2070, b2086
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yegS^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yegS
Mnemonic	Systematic nomenclature
Synonyms	ECK2082, b2086, JW2070^[1], JW2070
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	46.7 minutes	MG1655: 2166736..2167635 <gbrowseImage> name=NC_000913:2166736..2167635 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2123335..2124234 <gbrowseImage> name=NC_012967:2123335..2124234 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2059219..2060118 <gbrowseImage> name=NC_012759:2059219..2060118 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2170849..2171748 <gbrowseImage> name=NC_007779:2170849..2171748 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2257744..2258643 <gbrowseImage> name=NC_010473:2257744..2258643 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔyegS (Keio:JW2070)	deletion	deletion			PMID:16738554	Shigen CGSC9693^[4]
ΔyegS::kan	deletion		Biolog:respiration	unable to respire D-Mannose	PMID:16095938
ΔyegS::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔyegS783::kan					PMID:16738554	CGSC:104522
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2070	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAGAATTTCCCGCCAGCTT Primer 2:CCACGCAACAGTGGACAATCTGG
1H4	Kohara Phage	Genobase	PMID:3038334
10H4	Kohara Phage	Genobase	PMID:3038334
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[4]	est. P1 cotransduction: % ^[5] Synonyms:zee-3129::Tn10
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[4]	est. P1 cotransduction: 73% ^[5] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7123	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14367	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003708	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946626	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4111	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006911	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YegS
Synonyms	conserved protein^[1], B2086^[2]^[1], YegS^[2]^[1] , ECK2082, JW2070, b2086
Product description	conserved protein^[2]; lipid kinase^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01377	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0001727	lipid kinase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01377	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004143	diacylglycerol kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001206	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01377	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01377	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0007205	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001206	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008654	phospholipid biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01377	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008654	phospholipid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0594	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	fur	PMID:16606699	Experiment(s):EBI-1141881
Protein	napC	PMID:16606699	Experiment(s):EBI-1141881
Protein	nrdI	PMID:16606699	Experiment(s):EBI-1141881
Protein	nadE	PMID:16606699	Experiment(s):EBI-1141881
Protein	dapD	PMID:16606699	Experiment(s):EBI-1141881
Protein	gltA	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAEFPASLLI LNGKSTDNLP LREAIMLLRE EGMTIHVRVT WEKGDAARYV EEARKFGVAT VIAGGGDGTI NEVSTALIQC EGDDIPALGI LPLGTANDFA TSVGIPEALD KALKLAIAGD AIAIDMAQVN KQTCFINMAT GGFGTRITTE TPEKLKAALG SVSYIIHGLM RMDTLQPDRC EIRGENFHWQ GDALVIGIGN GRQAGGGQQL CPNALINDGL LQLRIFTGDE ILPALVSTLK SDEDNPNIIE GASSWFDIQA PHDITFNLDG EPLSGQNFHI EILPAALRCR LPPDCPLLR
Length	299
Mol. Wt	32.039 kDa
pI	4.5 (calculated)
Extinction coefficient	19,480 - 20,230 (calc based on 2 Y, 3 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	6..132	PF00781 Diacylglycerol kinase catalytic domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yegS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130026	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946626	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006911	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76407	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7123	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14367	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946626	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003708	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4111	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	18	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	170	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yegS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2166716..2166756 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2086 (EcoliWiki Page)	NCBI GEO profiles for yegS
microarray	GenExpDB:b2086 (EcoliWiki Page)	Summary of data for yegS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yegS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7123	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4111	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2086
EcoGene	EcoGene:EG14367	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003708	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006911	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G46090 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0220132 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA16938-PA (score: 1.000; bootstrap: 100%) GA14519-PA (score: 0.323)	From Inparanoid:20070104
Shigella flexneri	S2274	From SHIGELLACYC
E. coli O157	Z3252	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00781 Diacylglycerol kinase catalytic domain
Panther (EcoliWiki Page)	PTHR12358:SF4
EcoCyc	EcoCyc:G7123	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14367	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003708	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4111	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006911	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yegS:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yegS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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