yegE:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
yegE |
|---|---|
| Gene Synonym(s) |
ECK2061, b2067, JW2052[1], JW2052 |
| Product Desc. |
putative sensor-type protein[2][3] Putative c-di-GMP dual activity enzyme, function unknown[4] |
| Product Synonyms(s) |
predicted diguanylate cyclase, GGDEF domain signalling protein[1], B2067[2][1], YegE[2][1] , ECK2061, JW2052, b2067 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links |
|
| edit table |
</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
A yegE null mutation partially suppresses the motility defect of a yhjH mutation; yegE partial suppression is best at 28C, full yhjH suppression requires yegE and yedQ mutations; a yeaJ null mutation is a better yhjH suppressor at 37C; full yhjH suppression requires yegE, yedQ and yeaJ null mutations when grown at 37C (Pesavento, 2008). YegE has an N-terminal MASE1 and three PAS domains, then a diguanylate cyclase GGDEF domain, followed by a C-terminal cyclic-di-GMP phosphodiesterase EAL domain. RpoS regulon (Pesavento, 2008).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
yegE |
|---|---|
| Mnemonic |
Systematic nomenclature |
| Synonyms |
ECK2061, b2067, JW2052[1], JW2052 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
46.15 minutes |
MG1655: 2141290..2144607 |
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NC_012967: 2076472..2079777 |
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NC_012759: 2033773..2037090 |
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W3110 |
|
W3110: 2145403..2148720 |
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DH10B: 2232298..2235615 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔyegE (Keio:JW2052) |
deletion |
deletion |
PMID:16738554 |
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|
ΔyegE::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
PMID:16095938 |
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|
ΔyegE-765::kan |
PMID:16738554 |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2052 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAGCAAACAATCACAGCATGT Primer 2:CCGTTAATCGCGAAATAACTACT | |
|
Kohara Phage |
PMID:3038334 | ||
|
Kohara Phage |
PMID:3038334 | ||
|
zef-3129::Tn10 |
Linked marker |
est. P1 cotransduction: 5% [6] | |
|
Linked marker |
est. P1 cotransduction: 25% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002259 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EchoBASE:EB2297 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0006845 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
YegE |
|---|---|
| Synonyms |
predicted diguanylate cyclase, GGDEF domain signalling protein[1], B2067[2][1], YegE[2][1] , ECK2061, JW2052, b2067 |
| Product description |
putative sensor-type protein[2][3] Putative c-di-GMP dual activity enzyme, function unknown[4] |
| EC number (for enzymes) |
|
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
GO:0000155 |
two-component sensor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000700 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000700 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0004871 |
signal transducer activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000014 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001610 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000014 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0007165 |
signal transduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001610 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
plasma membrane |
C-terminus localized in the cytoplasm with 10 predicted transmembrane domains |
Daley et al. (2005) [7] |
| |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSKQSQHVLI ALPHPLLHLV SLGLVSFIFT LFSLELSQFG TQLAPLWFPT SIMMVAFYRH AGRMWPGIAL SCSLGNIAAS ILLFSTSSLN MTWTTINIVE AVVGAVLLRK LLPWYNPLQN LADWLRLALG SAIVPPLLGG VLVVLLTPGD DPLRAFLIWV LSESIGALAL VPLGLLFKPH YLLRHRNPRL LFESLLTLAI TLTLSWLSML YLPWPFTFII VLLMWSAVRL PRMEAFLIFL TTVMMVSLMM AADPSLLATP RTYLMSHMPW LPFLLILLPA NIMTMVMYAF RAERKHISES ETHFRNAMEY SAIGMALVGT EGQWLQTNKA LCQFLGYSQE ELRGLTFQQL TWPEDLNKDL QQVEKLISGE INTYSMEKRY YNRNGDVVWA LLAVSLVRHT DGTPLYFIAQ IEDINELKRT EQVNQQLMER ITLANEAGGI GIWEWELKPN IFSWDKRMFE LYEIPPHIKP NWQVWYECVL PEDRQHAEKV IRDSLQSRSP FKLEFRITVK DGIRHIRALA NRVLNKEGEV ERLLGINMDM TEVKQLNEAL FQEKERLHIT LDSIGEAVVC IDMAMKITFM NPVAEKMSGW TQEEALGVPL LTVLHITFGD NGPLMENIYS ADTSRSAIEQ DVVLHCRSGG SYDVHYSITP LSTLDGSNIG SVLVIQDVTE SRKMLRQLSY SASHDALTHL ANRASFEKQL RILLQTVNST HQRHALVFID LDRFKAVNDS AGHAAGDALL RELASLMLSM LRSSDVLARL GGDEFGLLLP DCNVESARFI ATRIISAVND YHFIWEGRVH RVGASAGITL IDDNNHQAAE VMSQADIACY ASKNGGRGRV TVYEPQQAAA HSERAAMSLD EQWRMIKENQ LMMLAHGVAS PRIPEARNLW LISLKLWSCE GEIIDEQTFR RSFSDPALSH ALDRRVFHEF FQQAAKAVAS KGISISLPLS VAGLSSATLV NDLLEQLENS PLPPRLLHLI IPAEAILDHA ESVQKLRLAG CRIVLSQVGR DLQIFNSLKA NMADYLLLDG ELCANVQGNL MDEMLITIIQ GHAQRLGMKT IAGPVVLPLV MDTLSGIGVD LIYGEVIADA QPLDLLVNSS YFAIN |
| Length |
1,105 |
| Mol. Wt |
123.891 kDa |
| pI |
6.2 (calculated) |
| Extinction coefficient |
162,260 - 163,510 (calc based on 24 Y, 23 W, and 10 C residues) |
| edit table |
Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/>
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Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
ASAP:ABE-0006845 |
Escherichia coli str. K-12 substr. MG1655 | |
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Escherichia coli str. K-12 substr. MG1655 | ||
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EcoCyc:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
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EcoGene:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
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RegulonDB:ECK120002259 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB2297 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
Ecoli K-12 |
2.345+/-0.116 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
|
mRNA |
Ecoli K-12 |
0.014470907 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
|
Protein |
E. coli K-12 MG1655 |
12 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
26 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
2a |
molecules/cell/generation |
|
Ribosome Profiling |
Low confidence in the sequencing data set. |
PMID: 24766808 |
| edit table |
Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
 Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2141270..2141310
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for yegE | |
|
microarray |
Summary of data for yegE from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
|
GFP Fusion |
Intergenic region (2140925..2141313) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
| edit table |
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Notes
Accessions Related to yegE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB2297 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002259 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0006845 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
|
E. coli O157 |
Z3236 |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG12396 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002259 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB2297 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0006845 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
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