ydiU:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

ydiU

Gene Synonym(s)

ECK1704, b1706, JW1696[1], JW1696

Product Desc.

conserved protein[2][3]

Function unknown[4]

Product Synonyms(s)

conserved protein[1], B1706[2][1], YdiU[2][1] , ECK1704, JW1696, b1706

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ydiU[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ydiU

Mnemonic

Systematic nomenclature

Synonyms

ECK1704, b1706, JW1696[1], JW1696

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.53 minutes 

MG1655: 1789268..1787832
<gbrowseImage> name=NC_000913:1787832..1789268 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1768512..1767076
<gbrowseImage> name=NC_012967:1767076..1768512 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1679891..1681327
<gbrowseImage> name=NC_012759:1679891..1681327 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1792958..1791522
<gbrowseImage> name=NC_007779:1791522..1792958 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1879839..1878403
<gbrowseImage> name=NC_010473:1878403..1879839 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔydiU (Keio:JW1696)

deletion

deletion

PMID:16738554

Shigen
CGSC9435[5]

ΔydiU780::kan

PMID:16738554

CGSC:104338


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1696

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACCCTGTCTTTTGTTACCCG

Primer 2:CCACTCGAGCAGCTGACTTCCAG

20F5

Kohara Phage

Genobase

PMID:3038334

20H8

Kohara Phage

Genobase

PMID:3038334

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 69% [6]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[5]

est. P1 cotransduction: 14% [6]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6924

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13980

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003512

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946219

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3737

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005694

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YdiU

Synonyms

conserved protein[1], B1706[2][1], YdiU[2][1] , ECK1704, JW1696, b1706

Product description

conserved protein[2][3]

Function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699

Experiment(s):EBI-1140553

Protein

ydiZ

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTLSFVTRWR DELPETYTAL SPTPLNNARL IWHNTELANT LSIPSSLFKN GAGVWGGEAL
LPGMSPLAQV YSGHQFGVWA GQLGDGRGIL LGEQLLADGT TMDWHLKGAG LTPYSRMGDG
RAVLRSTIRE SLASEAMHYL GIPTTRALSI VTSDSPVYRE TAEPGAMLMR VAPSHLRFGH
FEHFYYRRES EKVRQLADFA IRHYWSHLAD DEDKYRLWFS DVVARTASLI AQWQTVGFAH
GVMNTDNMSL LGLTLDYGPF GFLDDYEPGF ICNHSDHQGR YSFDNQPAVA LWNLQRLAQT
LSPFVAVDAL NEALDSYQQV LLTHYGERMR QKLGFMTEQK EDNALLNELF SLMARERSDY
TRTFRMLSLT EQHSAASPLR DEFIDRAAFD DWFARYRGRL QQDEVSDSER QQLMQSVNPA
LVLRNWLAQR AIEAAEKGDM TELHRLHEAL RNPFSDRDDD YVSRPPDWGK RLEVSCSS
Length

478

Mol. Wt

54.382 kDa

pI

5.7 (calculated)

Extinction coefficient

91,330 - 91,580 (calc based on 17 Y, 12 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..454

PF02696 Uncharacterized ACR, YdiU/UPF0061 family

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ydiU taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129662

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946219

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005694

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77649

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6924

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13980

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946219

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003512

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3737

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

205

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

153

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

153

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ydiU

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1789248..1789288 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GenExpDB:b1706 (EcoliWiki Page)

Summary of data for ydiU from multiple microarray studies

microarray

PMID: 16677314

ydiU is activated by IscR

reporter genes

PMID: 16677314

a chromosomal lacZ fusion to the ydiU promoter region was constructed by replacing the chromosomal lac promoter

microarray

GEO Profiles:b1706 (EcoliWiki Page)

NCBI GEO profiles for ydiU


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1789198..1789386) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ17; Well:A11[7]

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Notes

Accessions Related to ydiU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6924

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3737

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1706

EcoGene

EcoGene:EG13980

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003512

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005694

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000028394 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G13030 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000039297 (score: 1.000; bootstrap: 97%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000000988 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005632 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-041210-110 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000014135 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000248845 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000018531 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000006822 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1919007 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q5ZA96 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000041724 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000007695 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YPL222W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC20G45c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000129590 (score: 1.000; bootstrap: 69%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00015914001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000018757 (score: 1.000; bootstrap: 56%)
  • ENSXETP00000048181 (score: 1.000; bootstrap: 57%)

From Inparanoid:20070104

Shigella flexneri

S1642

From SHIGELLACYC

E. coli O157

Z2735

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Panther (EcoliWiki Page)

PTHR12153:SF15

Pfam (EcoliWiki Page)

PF02696 Uncharacterized ACR, YdiU/UPF0061 family

EcoCyc

EcoCyc:G6924

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13980

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003512

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3737

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005694

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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