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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	ybfL
Gene Synonym(s)	ECK0694, b0705, JW0695^[1], JW0695
Product Desc.	predicted transposase^[2]; predicted transposase; receptor protein^[3]
Product Synonyms(s)	B0705^[2], YbfL^[2] , ECK0694, JW0695, b0705
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ybfL^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ybfL
Mnemonic
Synonyms	ECK0694, b0705, JW0695^[1], JW0695
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	15.87 minutes	MG1655: 736123..737184 <gbrowseImage> name=NC_000913:736123..737184 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 737322..738383 <gbrowseImage> name=NC_007779:737322..738383 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 788640..789776 <gbrowseImage> name=NC_010473:788640..789776 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔybfL (Keio:JW0695)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0695	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCAGGCATCCTACTATTGAC Primer 2:CCCGAAAGCCCGCTCCCCGCAAG
3G6	Kohara Phage	Genobase	PMID:3038334
25C10S	Kohara Phage	Genobase	PMID:3038334
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[4]	est. P1 cotransduction: 23% ^[5] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
nadA57::Tn10	Linked marker	CAG12147 = CGSC7351^[4]	est. P1 cotransduction: 15% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13417	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002977	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947075	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3193	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002407	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YbfL
Synonyms	B0705^[2], YbfL^[2] , ECK0694, JW0695, b0705
Product description	predicted transposase^[2]; predicted transposase; receptor protein^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004803	transposase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006313	transposition, DNA-mediated	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ccmH	PMID:16606699	Experiment(s):EBI-1137727
Protein	ybgA	PMID:16606699	Experiment(s):EBI-1137727
Protein	ydcC	PMID:16606699	Experiment(s):EBI-1137727
Protein	yhhI	PMID:16606699	Experiment(s):EBI-1137727
Protein	dnaJ	PMID:16606699	Experiment(s):EBI-1137727
Protein	groL	PMID:16606699	Experiment(s):EBI-1137727
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRDCHSSNDK DVIAIDGKTL RHSYDKSRRR GAIHVISAFS TMHSLVIGQI KTDKKSNEIT AIPELLNMLD IKGKIITTDA MGCQKDIAEK IQKQGGDYLF AVKGNQGRLN KAFEEKFPLK ELNNPEHDSY AMSEKSHGRE EIRLHIVCDV PDELIDFTFE WKGLKKLCVA VSFRSIIAEQ KKEPEMTVRY YISSADLTAE KFATAIRNHW HVENKLHWRL DVVMNEDDCK IRRGNAAELF SGIRHIAINI LTNDKVFKAG LRRKMRKAAM DRNYLASVLA GSGLS
Length	285
Mol. Wt	32.417 kDa
pI	9.4 (calculated)
Extinction coefficient	25,440 - 26,065 (calc based on 6 Y, 3 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	100..343	PF01609 Transposase DDE domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ybfL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
ASAP	ASAP:ABE-0002407	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947075	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P75741	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13417	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947075	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002977	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3193	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ybfL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:736103..736143 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0705 (EcoliWiki Page)	NCBI GEO profiles for ybfL
microarray	GenExpDB:b0705 (EcoliWiki Page)	Summary of data for ybfL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ybfL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6377	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3193	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0705
EcoGene	EcoGene:EG13417	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002977	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002407	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000038119 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	Z2253	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01609 Transposase DDE domain
EcoCyc	EcoCyc:G6377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13417	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002977	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3193	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002407	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ybfL:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ybfL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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