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Standard Name	uxuB
Gene Synonym(s)	ECK4314, b4323, JW4286^[1], JW4286
Product Desc.	MANNONOXIDOREDUCT-
Product Synonyms(s)	D-mannonate oxidoreductase, NAD-binding^[1], B4323^[2]^[1], UxuB^[2]^[1], fructuronate reductase product^[2]^[1], D-mannonate:NAD+ 5-oxidoreductase^[2]^[1] , ECK4314, JW4286, b4323
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): uxuAB^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	uxuB
Mnemonic	Utilization of heXUronide
Synonyms	ECK4314, b4323, JW4286^[1], JW4286
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	98.09 minutes, 98.09 minutes	MG1655: 4550924..4552384 <gbrowseImage> name=NC_000913:4550924..4552384 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4533815..4535275 <gbrowseImage> name=NC_012967:4533815..4535275 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4489409..4490869 <gbrowseImage> name=NC_012759:4489409..4490869 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4557581..4559041 <gbrowseImage> name=NC_007779:4557581..4559041 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
uxuB(del) (Keio:JW4286)	deletion	deletion	PMID:16738554	Shigen CGSC11071^[3]
uxuB108			PMID:4603847	CGSC:6450
uxuB720(del)::kan			PMID:16738554	CGSC:103360
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4286	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACTACTATTGTTGACAGCAA Primer 2:CCaAGCGCAGCCACACACTCGCG
1D6	Kohara Phage	Genobase	PMID:3038334
5G8	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[3]	est. P1 cotransduction: % ^[4] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[3]	est. P1 cotransduction: 16% ^[4] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG20248	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20248	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002446	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946795	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4150	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014175	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	UxuB
Synonyms	D-mannonate oxidoreductase, NAD-binding^[1], B4323^[2]^[1], UxuB^[2]^[1], fructuronate reductase product^[2]^[1], D-mannonate:NAD+ 5-oxidoreductase^[2]^[1] , ECK4314, JW4286, b4323
Product description	MANNONOXIDOREDUCT-
EC number (for enzymes)	1.1.1.57^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005488	binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008866	fructuronate reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.1.1.57	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050662	coenzyme binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013118	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050662	coenzyme binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013328	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000669	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013118	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	phoR	PMID:16606699	Experiment(s):EBI-1147864
Protein	nadE	PMID:16606699	Experiment(s):EBI-1147864
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTTIVDSNLP VARPSWDHSR LESRIVHLGC GAFHRAHQAL YTHHLLESTD SDWGICEVNL MPGNDRVLIE NLKKQQLLYT VAEKGAESTE LKIIGSMKEA LHPEIDGCEG ILNAMARPQT AIVSLTVTEK GYCADAASGQ LDLNNPLIKH DLENPTAPKS AIGYIVEALR LRREKGLKAF TVMSCDNVRE NGHVAKVAVL GLAQARDPQL AAWIEENVTF PCTMVDRIVP AATPETLQEI ADQLGVYDPC AIACEPFRQW VIEDNFVNGR PDWDKVGAQF VADVVPFEMM KLRMLNGSHS FLAYLGYLGG YETIADTVTN PAYRKAAFAL MMQEQAPTLS MPEGTDLNAY ATLLIERFSN PSLRHRTWQI AMDGSQKLPQ RLLDPVRLHL QNGGSWRHLA LGVAGWMRYT QGVDEQGNAI DVVDPMLAEF QKINAQYQGA DRVKALLGLS GIFADDLPQN ADFVGAVTAA YQQLCERGAR ECVAAL
Length	486
Mol. Wt	53.58 kDa
pI	5.3 (calculated)
Extinction coefficient	63,370 - 64,620 (calc based on 13 Y, 8 W, and 10 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	23..188	PF01232 Mannitol dehydrogenase Rossmann domain	PMID:19920124
Domain	216..463	PF08125 Mannitol dehydrogenase C-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=uxuB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132144	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946795	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014175	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39160	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG20248	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20248	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946795	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002446	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4150	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	364	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	90	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	213	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
uxuAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4550904..4550944 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4323 (EcoliWiki Page)	NCBI GEO profiles for uxuB
microarray	GenExpDB:b4323 (EcoliWiki Page)	Summary of data for uxuB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to uxuB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG20248	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4150	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4323
EcoGene	EcoGene:EG20248	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002446	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014175	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Saccharomyces cerevisiae	YEL070W (score: 1.000; bootstrap: 100%) YNR073C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000040196 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	UXUB	From SHIGELLACYC
E. coli O157	UXUB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01232 Mannitol dehydrogenase Rossmann domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:48179
Superfamily (EcoliWiki Page)	SUPERFAMILY:51735
Pfam (EcoliWiki Page)	PF08125 Mannitol dehydrogenase C-terminal domain
EcoCyc	EcoCyc:EG20248	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20248	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002446	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4150	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014175	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 CGSC: The Coli Genetics Stock Center
↑ ^4.0 ^4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

uxuB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to uxuB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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