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Expression|

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Standard Name	uxuA
Gene Synonym(s)	ECK4313, b4322, JW4285^[1], JW4285
Product Desc.	MANNONDEHYDRAT- D-Mannonate dehydratase (hydrolase); Fe(2)-requiring; glucuronate branch of hexuronate pathway^[2]
Product Synonyms(s)	mannonate hydrolase^[1], B4322^[3]^[1], UxuA^[3]^[1], D-mannonate hydro-lyase^[3]^[1] , ECK4313, JW4285, b4322
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): uxuAB^[3]
Regulation/Activity
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Notes

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UxuR regulon.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	uxuA
Mnemonic	Utilization of heXUronide
Synonyms	ECK4313, b4322, JW4285^[1], JW4285
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	98.06 minutes	MG1655: 4549659..4550843 <gbrowseImage> name=NC_000913:4549659..4550843 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4532550..4533734 <gbrowseImage> name=NC_012967:4532550..4533734 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4488144..4489328 <gbrowseImage> name=NC_012759:4488144..4489328 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4556316..4557500 <gbrowseImage> name=NC_007779:4556316..4557500 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
uxuA(del) (Keio:JW4285)	deletion	deletion		PMID:16738554	Shigen CGSC11070^[4]
uxuAI52V		I52V	(in strain: MT78)			Strain variation; seeded from UniProt:P24215
uxuA1					CGSC:6439
uxuA790(del)::kan				PMID:16738554	CGSC:103358
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4285	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAACAGACCTGGCGCTGGTA Primer 2:CCACGGCTAAAGAAAGCGCGCTG
3F1	Kohara Phage	Genobase	PMID:3038334
1D6	Kohara Phage	Genobase	PMID:3038334
5G8	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[4]	est. P1 cotransduction: % ^[5] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[4]	est. P1 cotransduction: 15% ^[5] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11066	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11066	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001055	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947082	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1059	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	UxuA
Synonyms	mannonate hydrolase^[1], B4322^[3]^[1], UxuA^[3]^[1], D-mannonate hydro-lyase^[3]^[1] , ECK4313, JW4285, b4322
Product description	MANNONDEHYDRAT- D-Mannonate dehydratase (hydrolase); Fe(2)-requiring; glucuronate branch of hexuronate pathway^[2]
EC number (for enzymes)	4.2.1.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008927	mannonate dehydratase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00106	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008927	mannonate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004628	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008927	mannonate dehydratase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.8	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	nadE		PMID:16606699	Experiment(s):EBI-1147861
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR
Length	394
Mol. Wt	44.838 kDa
pI	5.5 (calculated)
Extinction coefficient	56,380 - 56,755 (calc based on 12 Y, 7 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..389	PF03786 D-mannonate dehydratase (UxuA)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=uxuA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132143	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947082	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014173	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P24215	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11066	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11066	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947082	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001055	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1059	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	595	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	144	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	325	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
uxuAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4549639..4549679 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4322 (EcoliWiki Page)	NCBI GEO profiles for uxuA
microarray	GenExpDB:b4322 (EcoliWiki Page)	Summary of data for uxuA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4548769..4549274) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ15; Well:H9^[6]
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Notes

Accessions Related to uxuA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11066	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1059	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4322
EcoGene	EcoGene:EG11066	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001055	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	UXUA	From SHIGELLACYC
E. coli O157	UXUA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:51658
Pfam (EcoliWiki Page)	PF03786 D-mannonate dehydratase (UxuA)
EcoCyc	EcoCyc:EG11066	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11066	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001055	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1059	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

uxuA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to uxuA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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