umuD:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
umuD |
|---|---|
| Mnemonic |
UV mutator |
| Synonyms |
ECK1171, b1183, JW1172, uvm[1] |
| edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
26.51 minutes |
MG1655: 1229990..1230409 |
||
|
NC_012967: 1230917..1231336 |
||||
|
NC_012759: 1118711..1119130 |
||||
|
W3110 |
|
W3110: 1232344..1232763 |
||
|
DH10B: 1270231..1270650 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
umuD(del) (Keio:JW1172) |
deletion |
deletion |
|||||
|
umuD::Tn5KAN-2 (FB20262) |
Insertion at nt 77 in Minus orientation |
does not contain pKD46 | |||||
|
umuDP27D |
P27D |
In umuD1; non-cleavable |
seeded from UniProt:P0AG11 | ||||
|
umuDG65R |
G65R |
In umuD44; non-cleavable |
seeded from UniProt:P0AG11 | ||||
|
umuDG92D |
G92D |
In umuD77; non-cleavable |
seeded from UniProt:P0AG11 | ||||
|
umuD44 |
|||||||
|
umuD77 |
|||||||
|
umuD1 |
|||||||
|
umuD772(del)::kan |
|||||||
|
umuDC D8N |
decreased levels of the Beta processitvity clamp |
D to N |
Sensitivity to |
There is a decrease in the sensitivity to cold. |
See Figure 1 | ||
|
(umuDC)595(del)::cat |
Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat) |
Sensitivity to |
Sensitivity toward UV radiation was moderately increased. |
RW82 7280 |
See Figure 1. | ||
|
polB(del)umuDC(del) |
Deletion of araD-polB operon and replaced with omega cassette. Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat) |
Resistant to |
Sensitivity toward UV radiation was increased by 2 to 3 fold. |
See Figure 1. | |||
|
(umuDC)595(del)::cat |
Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat) |
Rate of replication |
there was a slight delay in the resumption of DNA replication after exposure to UV radiation. |
RW82 7280 |
See Figure 2 | ||
|
polB(del)umuDC(del) |
Deletion of araD-polB operon and replaced with omega cassette. Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat) |
rate of replication |
There was a significant delay in the resumption of DNA replication after exposure to UV radiation. |
See Figure 3. | |||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1172 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTTGTTTATCAAGCCTGCGGA Primer 2:CCGCGCATCGCCTTAACGACGTG | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 50% [9] | ||
|
Linked marker |
est. P1 cotransduction: 87% [9] | ||
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Beuning, PJ et al. (2009) Characterization of novel alleles of the Escherichia coli umuDC genes identifies additional interaction sites of UmuC with the beta clamp. J. Bacteriol. 191 5910-20 PubMed
- ↑ 6.0 6.1 6.2 6.3 Rangarajan, S et al. (1999) A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 96 9224-9 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
