uhpB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

uhpB

Gene Synonym(s)

ECK3659, b3668, JW3643, uhpR[1]

Product Desc.

UhpB[2][3]

Membrane protein controlling UhpA activity, sensor kinase[4]

Product Synonyms(s)

B3668[2], sensor protein UhpB[2], sensor kinase-phosphotransferase UhpB[2], unphosphorylated sensor kinase-phosphotransferase UhpB[2], unmodified sensor kinase-phosphotransferase UhpB[2] , ECK3659, JW3643, b3668

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): uhpABC[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Probable false positive lipoprotein prediction, Cys not conserved, 10 CM TMs predicted.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uhpB

Mnemonic

Utilization hexose phosphate

Synonyms

ECK3659, b3668, JW3643, uhpR[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

82.91 minutes 

MG1655: 3848159..3846657
<gbrowseImage> name=NC_000913:3846657..3848159 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3810068..3808566
<gbrowseImage> name=NC_012967:3808566..3810068 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3734990..3736492
<gbrowseImage> name=NC_012759:3734990..3736492 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3790279..3791781
<gbrowseImage> name=NC_007779:3790279..3791781 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3945743..3944241
<gbrowseImage> name=NC_010473:3944241..3945743 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔuhpB (Keio:JW3643)

deletion

deletion

PMID:16738554

Shigen
CGSC10674[5]

uhpB::Tn5KAN-I-SceI (FB21381)

Insertion at nt 917 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21381

contains pKD46

uhpB::Tn5KAN-I-SceI (FB21382)

Insertion at nt 917 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21382

does not contain pKD46

ΔuhpB773::kan

PMID:16738554

CGSC:103292


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3643

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAGACGTTGTTCTCCCGCTT

Primer 2:CCGACATAGCGTTGAGGTAGAGA

7C4

Kohara Phage

Genobase

PMID:3038334

1D11

Kohara Phage

Genobase

PMID:3038334

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[5]

est. P1 cotransduction: 50% [6]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 35% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11052

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001041

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948195

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1045

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011993

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

UhpB

Synonyms

B3668[2], sensor protein UhpB[2], sensor kinase-phosphotransferase UhpB[2], unphosphorylated sensor kinase-phosphotransferase UhpB[2], unmodified sensor kinase-phosphotransferase UhpB[2] , ECK3659, JW3643, b3668

Product description

UhpB[2][3]

Membrane protein controlling UhpA activity, sensor kinase[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

F

Seeded from EcoCyc (v14.0)

complete

GO:0000155

two-component sensor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011712

F

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011712

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0902

P

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004673

protein histidine kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

F

Seeded from EcoCyc (v14.0)

complete

GO:0004673

protein histidine kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.13.3

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003594

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0007165

signal transduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011712

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0018106

peptidyl-histidine phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005467

P

Seeded from EcoCyc (v14.0)

complete

GO:0046983

protein dimerization activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011712

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MMKTLFSRLI TVIACFFIFS AAWFCLWSIS LHLVERPDMA VLLFPFGLRL GLMLQCPRGY
WPVLLGAEWL LIYWLTQAVG LTHFPLLMIG SLLTLLPVAL ISRYRHQRDW RTLLLQGAAL
TAAALLQSLP WLWHGKESWN ALLLTLTGGL TLAPICLVFW HYLANNTWLP LGPSLVSQPI
NWRGRHLVWY LLLFVISLWL QLGLPDELSR FTPFCLALPI IALAWHYGWQ GALIATLMNA
IALIASQTWR DHPVDLLLSL LVQSLTGLLL GAGIQRLREL NQSLQKELAR NQHLAERLLE
TEESVRRDVA RELHDDIGQT ITAIRTQAGI VQRLAADNAS VKQSGQLIEQ LSLGVYDAVR
RLLGRLRPRQ LDDLTLEQAI RSLMREMELE GRGIVSHLEW RIDESALSEN QRVTLFRVCQ
EGLNNIVKHA DASAVTLQGW QQDERLMLVI EDDGSGLPPG SGQQGFGLTG MRERVTALGG
TLHISCLHGT RVSVSLPQRY V
Length

501

Mol. Wt

56.438 kDa

pI

8.1 (calculated)

Extinction coefficient

116,420 - 117,295 (calc based on 8 Y, 19 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

409..498

PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

PMID:19920124

Domain

10..277

PF05231 MASE1

PMID:19920124

Domain

304..370

PF07730 Histidine kinase

PMID:19920124

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<motif_map/>

tmhmm.php?gene=uhpB&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=uhpB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111633

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948195

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011993

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09835

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11052

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11052

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948195

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001041

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1045

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

32

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

14

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

uhpABC

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3848139..3848179 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3668 (EcoliWiki Page)

NCBI GEO profiles for uhpB

microarray

GenExpDB:b3668 (EcoliWiki Page)

Summary of data for uhpB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to uhpB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11052

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1045

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3668

EcoGene

EcoGene:EG11052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001041

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011993

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

UHPB

From SHIGELLACYC

E. coli O157

UHPB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Panther (EcoliWiki Page)

PTHR24423:SF339

Superfamily (EcoliWiki Page)

SUPERFAMILY:55874

Pfam (EcoliWiki Page)

PF05231 MASE1

Pfam (EcoliWiki Page)

PF07730 Histidine kinase

EcoCyc

EcoCyc:EG11052

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001041

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1045

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011993

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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