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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	tyrA
Gene Synonym(s)	ECK2597, b2600, JW2581^[1], JW2581
Product Desc.	TyrA^[2]^[3]; Component of CHORISMUTPREPHENDEHYDROG-CPLX^[2] Tyrosine synthesis: chorismate mutase/prephenate dehydratase^[4]
Product Synonyms(s)	fused chorismate mutase T^[1], prephenate dehydrogenase^[1], B2600^[2]^[1], TyrA^[2]^[1] , ECK2597, JW2581, b2600
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aroF-tyrA^[2], OP00010, aroF
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Bifunctional protein, mutase is N-terminal domain. TyrR regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tyrA
Mnemonic	Tyrosine
Synonyms	ECK2597, b2600, JW2581^[1], JW2581
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.99 minutes, 58.99 minutes	MG1655: 2738091..2736970 <gbrowseImage> name=NC_000913:2736970..2738091 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2661476..2660355 <gbrowseImage> name=NC_012967:2660355..2661476 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2622782..2623903 <gbrowseImage> name=NC_012759:2622782..2623903 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2738725..2737604 <gbrowseImage> name=NC_007779:2737604..2738725 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2829856..2828735 <gbrowseImage> name=NC_010473:2828735..2829856 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
tyrA(del) (Keio:JW2581)	deletion	deletion	PMID:16738554	Shigen CGSC10049^[5]
tyrA::Tn5KAN-I-SceI (FB20865)	Insertion at nt 491 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20865	contains pKD46
tyrA4				CGSC:4851
tyrA2				CGSC:5299
tyrA14				CGSC:7359
tyrA15(Am)				CGSC:8790	amber (UAG) mutation
tyrA16::Tn10				CGSC:8868
tyrA6				CGSC:8934
tyrA1::Mu				CGSC:23654
tyrA117::Mu cts				CGSC:73503
tyrA763(del)::kan			PMID:16738554	CGSC:103260
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2581	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGTTGCTGAATTGACCGCATT Primer 2:CCtTGGCGATTGTCATTCGCCTG
E8F2	Kohara Phage	Genobase	PMID:3038334
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 35% ^[6]
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: 99% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001028	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947115	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008547	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TyrA
Synonyms	fused chorismate mutase T^[1], prephenate dehydrogenase^[1], B2600^[2]^[1], TyrA^[2]^[1] , ECK2597, JW2581, b2600
Product description	TyrA^[2]^[3]; Component of CHORISMUTPREPHENDEHYDROG-CPLX^[2] Tyrosine synthesis: chorismate mutase/prephenate dehydratase^[4]
EC number (for enzymes)	5.4.99.5^[1] 1.3.1.12^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004106	chorismate mutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008244	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011277	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.4.99.5	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004665	prephenate dehydrogenase (NADP+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003099	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008244	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011277	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006571	tyrosine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003099	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006571	tyrosine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008244	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006571	tyrosine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011277	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006571	tyrosine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0827	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008977	prephenate dehydrogenase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008244	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008977	prephenate dehydrogenase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.3.1.12	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042803	protein homodimerization activity	PMID:6395895	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008244	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011277	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of CHORISMUTPREPHENDEHYDROG-CPLX	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MVAELTALRD QIDEVDKALL NLLAKRLELV AEVGEVKSRF GLPIYVPERE ASMLASRRAE AEALGVPPDL IEDVLRRVMR ESYSSENDKG FKTLCPSLRP VVIVGGGGQM GRLFEKMLTL SGYQVRILEQ HDWDRAADIV ADAGMVIVSV PIHVTEQVIG KLPPLPKDCI LVDLASVKNG PLQAMLVAHD GPVLGLHPMF GPDSGSLAKQ VVVWCDGRKP EAYQWFLEQI QVWGARLHRI SAVEHDQNMA FIQALRHFAT FAYGLHLAEE NVQLEQLLAL SSPIYRLELA MVGRLFAQDP QLYADIIMSS ERNLALIKRY YKRFGEAIEL LEQGDKQAFI DSFRKVEHWF GDYAQRFQSE SRVLLRQAND NRQ
Length	373
Mol. Wt	42.042 kDa
pI	5.9 (calculated)
Extinction coefficient	42,400 - 42,775 (calc based on 10 Y, 5 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	124..345	PF02153 Prephenate dehydrogenase	PMID:19920124
Domain	9..89	PF01817 Chorismate mutase type II	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tyrA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130521	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947115	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008547	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P07023	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11039	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947115	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001028	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1032	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	12.826+/-0.092	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.037466548	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	610	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	561	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	446	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aroF-tyrA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2738071..2738111 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2600 (EcoliWiki Page)	NCBI GEO profiles for tyrA
microarray	GenExpDB:b2600 (EcoliWiki Page)	Summary of data for tyrA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to tyrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11039	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1032	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2600
EcoGene	EcoGene:EG11039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001028	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008547	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000026463 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G34930 (score: 1.000; bootstrap: 100%) AT1G15710 (score: 0.270)	From Inparanoid:20070104
Oryza gramene	Q5Z9H3 (score: 1.000; bootstrap: 100%) Q5Z9H5 (score: 0.633) Q5Z6Y1 (score: 0.381)	From Inparanoid:20070104
Shigella flexneri	TYRA	From SHIGELLACYC
E. coli O157	TYRA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01817 Chorismate mutase type II
Pfam (EcoliWiki Page)	PF02153 Prephenate dehydrogenase
Superfamily (EcoliWiki Page)	SUPERFAMILY:48179
Superfamily (EcoliWiki Page)	SUPERFAMILY:48600
Panther (EcoliWiki Page)	PTHR21363:SF0
EcoCyc	EcoCyc:EG11039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001028	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008547	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

tyrA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tyrA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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