trpB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
trpB |
|---|---|
| Mnemonic |
Tryptophan |
| Synonyms |
ECK1255, b1261, JW1253[1], JW1253 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
28.35 minutes |
MG1655: 1316439..1315246 |
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NC_012967: 1316009..1314816 |
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NC_012759: 4179047..4180240 |
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NC_012759: 1206095..1207288 |
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W3110 |
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W3110: 1320129..1318936 |
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DH10B: 1405835..1404642 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1315249 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
trpB(del) (Keio:JW1253) |
deletion |
deletion |
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trpB::Tn5KAN-2 (FB20277) |
Insertion at nt 776 in Minus orientation |
does not contain pKD46 | |||||
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trpBG281R |
G281R |
(in mutant TRPB8) |
Strain variation; seeded from UniProt:P0A879 | ||||
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trpB114::Tn10 |
Auxotrophies |
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trpB202 |
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trpB9579(Oc) |
Auxotrophies |
requires tryptophan for growth |
ochre (UAA) mutation | ||||
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trpB0 |
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trpB9601(Am) |
Auxotrophies |
amber (UAG) mutation | |||||
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trpB9676(Oc) |
Auxotrophies |
requires tryptophan for growth |
ochre (UAA) mutation | ||||
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trpB769(del)::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1253 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACAACATTACTTAACCCCTA Primer 2:CCGATTTCCCCTCGTGCTTTCAA | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 67% [12] | ||
|
Linked marker |
est. P1 cotransduction: 92% [12] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Crawford, IP et al. (1978) Assignment of the ends of the beta-chain of E. coli tryptophan synthase to the F1 and F2 domains. Biochem. Biophys. Res. Commun. 85 309-16 PubMed
- ↑ Ahmed, SA et al. (1986) Identification of three sites of proteolytic cleavage in the hinge region between the two domains of the beta 2 subunit of tryptophan synthase of Escherichia coli or Salmonella typhimurium. Biochemistry 25 3118-24 PubMed
- ↑ Higgins, W et al. (1980) Location of three active site residues in the NH2-terminal sequence of the beta 2 subunit tryptophan synthase from Escherichia coli. J. Biol. Chem. 255 512-7 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 9.0 9.1 9.2 Yanofsky, C & Ito, J (1966) Nonsense codons and polarity in the tryptophan operon. J. Mol. Biol. 21 313-34 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
