trpB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TrpB

Synonyms

tryptophan synthase, beta subunit[1], B1261[2][1], TrpB[2][1], β subunit[2][1] , ECK1255, JW1253, b1261

Product description

tryptophan synthase, β subunit[2][3];

Component of tryptophan synthase, β subunit dimer[3]; tryptophan synthase[2][3]

Tryptophan synthase, beta subunit[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000162

tryptophan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00133

P

Seeded from EcoCyc (v14.0)

complete

GO:0000162

tryptophan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0822

P

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001926

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00133

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006653

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006654

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.1.20

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:378665[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0456

F

Seeded from EcoCyc (v14.0)

complete

GO:0030170

pyridoxal phosphate binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001926

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:4887511[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005515

protein binding

PMID:378665[5]

IDA: Inferred from Direct Assay

F

Tryptophan synthase is a tetramer consisting of a dimer of alpha and a dimer of beta subunits. EcoliWiki:trpA|EcoliWiki:trpB

complete

GO:0030170

pyridoxal phosphate binding

PMID:350880[7]

IDA: Inferred from Direct Assay

F

complete

Contributes to

GO:0004834

tryptophan synthase activity

PMID:8325869[8]

IDA: Inferred from Direct Assay

F

complete

GO:0009073

aromatic amino acid family biosynthetic process

PMID:8325869[8]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000162

tryptophan biosynthetic process

PMID:8325869[8]

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of tryptophan synthase, β subunit dimer

could be indirect

Protein

paaJ

PMID:16606699[9]

Experiment(s):EBI-1139350

Protein

nadE

PMID:16606699[9]

Experiment(s):EBI-1139350

Protein

greB

PMID:16606699[9]

Experiment(s):EBI-1139350

Protein

lacZ

PMID:16606699[9]

Experiment(s):EBI-1139350

Protein

topA

PMID:16606699[9]

Experiment(s):EBI-1139350

Protein

Subunits of tryptophan synthase

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTTLLNPYFG EFGGMYVPQI LMPALRQLEE AFVSAQKDPE FQAQFNDLLK NYAGRPTALT
KCQNITAGTN TTLYLKREDL LHGGAHKTNQ VLGQALLAKR MGKTEIIAET GAGQHGVASA
LASALLGLKC RIYMGAKDVE RQSPNVFRMR LMGAEVIPVH SGSATLKDAC NEALRDWSGS
YETAHYMLGT AAGPHPYPTI VREFQRMIGE ETKAQILERE GRLPDAVIAC VGGGSNAIGM
FADFINETNV GLIGVEPGGH GIETGEHGAP LKHGRVGIYF GMKAPMMQTE DGQIEESYSI
SAGLDFPSVG PQHAYLNSTG RADYVSITDD EALEAFKTLC LHEGIIPALE SSHALAHALK
MMRENPDKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI
Length

397

Mol. Wt

42.983 kDa

pI

6.1 (calculated)

Extinction coefficient

23,380 - 24,005 (calc based on 12 Y, 1 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A879

Domain

50..378

PF00291 Pyridoxal-phosphate dependent enzyme

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=trpB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129222

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945768

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004234

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A879

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11025

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11025

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945768

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001014

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1018

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Gschwind, HP et al. (1979) Affinity chromatography of tryptophan synthase from Escherichia coli. Systematic studies with immobilized tryptophanol phosphate. Eur. J. Biochem. 96 403-16 PubMed
  6. ↑ Murphy, TM & Mills, SE (1969) Immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of Enterobacteriaceae. J. Bacteriol. 97 1310-20 PubMed
  7. ↑ Higgins, W & Miles, EW (1978) Affinity labeling of the pyridoxal phosphate binding site of the beta2 subunit of Escherichia coli tryptophan synthase. J. Biol. Chem. 253 4648-52 PubMed
  8. ↑ 8.0 8.1 8.2 Zhao, GP & Somerville, RL (1993) A single amino acid switch within the "hinge" region of the tryptophan synthase beta subunit of Escherichia coli that leads to diminished association with alpha subunit and arrested conversion of ESII to product. J. Biol. Chem. 268 14921-31 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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