tnaA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tnaA

Mnemonic

Tryptophanase

Synonyms

ECK3701, b3708, JW3686, ind, tnaR[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.77 minutes 

MG1655: 3886753..3888168
<gbrowseImage> name=NC_000913:3886753..3888168 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3849091..3850506
<gbrowseImage> name=NC_012967:3849091..3850506 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3775086..3776501
<gbrowseImage> name=NC_012759:3775086..3776501 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3750486..3749071
<gbrowseImage> name=NC_007779:3749071..3750486 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3984337..3985752
<gbrowseImage> name=NC_010473:3984337..3985752 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3886753

Edman degradation

PMID:9298646[2]
PMID:9732527[3]
PMID:10094700[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tnaA(del) (Keio:JW3686)

deletion

deletion

PMID:16738554[5]

Shigen

CGSC8309[6]

tnaAC294S

C294S

Identical to wild-type

seeded from UniProt:P0A853

tnaAC298S

C298S

Alters activity

seeded from UniProt:P0A853

tnaA5

CGSC:6240

tnaA6

CGSC:6257

[tnaA300]::Tn10

CGSC:9639

null allele

tnaA1

CGSC:9715

tnaA4

CGSC:11509

tnaA3

CGSC:11522

tnaA739(del)::kan

PMID:16738554[5]

CGSC:99778


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3686

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGAAAACTTTAAACATCTCCC

Primer 2:CCAACTTCTTTAAGTTTTGCGGT

2A2

Kohara Phage

Genobase

PMID:3038334[8]

3F11

Kohara Phage

Genobase

PMID:3038334[8]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[6]

est. P1 cotransduction: 64% [9]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[6]

est. P1 cotransduction: 15% [9]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11005

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11005

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000994

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948221

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0998

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012133

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. Rompf, A et al. (1998) Changes in protein synthesis as a consequence of heme depletion in Escherichia coli. Curr. Microbiol. 37 226-30 PubMed
  4. Blankenhorn, D et al. (1999) Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J. Bacteriol. 181 2209-16 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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