thrA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

thrA

Gene Synonym(s)

ECK0002, b0002, JW0001, Hs, thrA1, thrA2, thrD[1], thrD

Product Desc.

ThrA[2][3];

Component of aspartate kinase I[2]

Aspartokinase I and homoserine dehydrogenase I, bifunctional[4]

Product Synonyms(s)

fused aspartokinase I[1], homoserine dehydrogenase I[1], B0002[2][1], ThrD[2][1], ThrA[2][1] , ECK0002, Hs, JW0001, thrA1, thrA2, thrD, b0002

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thrLABC[2], OP00248, thr, thrABC

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrA

Mnemonic

Threonine

Synonyms

ECK0002, b0002, JW0001, Hs, thrA1, thrA2, thrD[1], thrD

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.01 minutes 

MG1655: 337..2799
<gbrowseImage> name=NC_000913:337..2799 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 336..2798
<gbrowseImage> name=NC_012967:336..2798 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 337..2799
<gbrowseImage> name=NC_012759:337..2799 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 337..2799
<gbrowseImage> name=NC_007779:337..2799 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 337..2799
<gbrowseImage> name=NC_010473:337..2799 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

337

Edman degradation

PMID:354697
PMID:4562989


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

thrA(del) (Keio:JW0001)

deletion

deletion

PMID:16738554

Shigen

thrA34::Tn10

Insertion at 425 bp in MG1655 (NC_000913)

adapted from Nichols et al.

thrA34::Tn10

CGSC:4834

thrA1101

CGSC:5343

thrA1015

CGSC:5352

thrA1100

CGSC:5879

thrA3092::Tn10kan

PMID:2540407 PMID:9829956

CGSC:38986

thrA1016

CGSC:73731


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0001

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCGAGTGTTGAAGTTCGGCGG

Primer 2:CCGACTCCTAACTTCCATGAGAG

9E4

Kohara Phage

Genobase

PMID:3038334

15A5

Kohara Phage

Genobase

PMID:3038334

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 28% [6]
Synonyms:car-96::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[5]

est. P1 cotransduction: 12% [6]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10998

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10998

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000987

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945803

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0991

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000008

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThrA

Synonyms

fused aspartokinase I[1], homoserine dehydrogenase I[1], B0002[2][1], ThrD[2][1], ThrA[2][1] , ECK0002, Hs, JW0001, thrA1, thrA2, thrD, b0002

Product description

ThrA[2][3];

Component of aspartate kinase I[2]

Aspartokinase I and homoserine dehydrogenase I, bifunctional[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc (v14.0)

complete

GO:0004072

aspartate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001341

F

Seeded from EcoCyc (v14.0)

complete

GO:0004072

aspartate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011147

F

Seeded from EcoCyc (v14.0)

complete

GO:0004072

aspartate kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018042

F

Seeded from EcoCyc (v14.0)

complete

GO:0004072

aspartate kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.2.4

F

Seeded from EcoCyc (v14.0)

complete

GO:0004412

homoserine dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011147

F

Seeded from EcoCyc (v14.0)

complete

GO:0004412

homoserine dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.3

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0009088

threonine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0791

P

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005106

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0016597

amino acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0050661

NADP or NADPH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005106

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011147

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of aspartate kinase I

could be indirect

Protein

glpD

PMID:16606699

Experiment(s):EBI-1135292

Protein

zwf

PMID:16606699

Experiment(s):EBI-1135292

Protein

ahpF

PMID:16606699

Experiment(s):EBI-1135292
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:4562989, PMID:354697, PMID:7003595, PMID:6777249

EchoLocation:thrA


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA
LPNISDAERI FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA
ALICRGEKMS IAIMAGVLEA RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP
ADHMVLMAGF TAGNEKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV
PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASRD
EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT QSSSEYSISF
CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL
ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL
LEQLKRQQSW LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL
VKEYHLLNPV IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM KFSGILSGSL SYIFGKLDEG MSFSEATTLA
REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE IEPVLPAEFN AEGDVAAFMA
NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK VKNGENALAF
YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV
Length

820

Mol. Wt

89.121 kDa

pI

5.5 (calculated)

Extinction coefficient

51,800 - 53,300 (calc based on 20 Y, 4 W, and 12 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..284

PF00696 Amino acid kinase family

PMID:19920124

Domain

320..379

PF01842 ACT domain

PMID:19920124

Domain

400..457

PF01842 ACT domain

PMID:19920124

Domain

614..811

PF00742 Homoserine dehydrogenase

PMID:19920124

Domain

472..606

PF03447 Homoserine dehydrogenase, NAD binding domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=thrA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16127996

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000008

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00561

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10998

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10998

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945803

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000987

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945803

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0991

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> ===Overview===This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

8.81E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

3789

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

5761

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4395

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thrLABC

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:317..357 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GenExpDB:b0002 (EcoliWiki Page)

Summary of data for thrA from multiple microarray studies

microarray

GEO Profiles:b0002 (EcoliWiki Page)

NCBI GEO profiles for thrA

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to thrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10998

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0991

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0002

EcoGene

EcoGene:EG10998

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000987

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000008

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT4G19710 (score: 1.000; bootstrap: 100%)
  • AT1G31230 (score: 0.669)

From Inparanoid:20070104

Oryza gramene

  • P49079 (score: 1.000; bootstrap: 100%)
  • Q6ZD10 (score: 0.834)
  • Q69LG7 (score: 0.772)
  • P93402 (score: 0.768)
  • P49080 (score: 0.766)
  • Q6ZD09 (score: 0.644)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YJR139C (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC7763 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000017647 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

THRA

From SHIGELLACYC

E. coli O157

THRA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01842 ACT domain

Pfam (EcoliWiki Page)

PF01842 ACT domain

Pfam (EcoliWiki Page)

PF00696 Amino acid kinase family

Pfam (EcoliWiki Page)

PF03447 Homoserine dehydrogenase, NAD binding domain

Pfam (EcoliWiki Page)

PF00742 Homoserine dehydrogenase

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Panther (EcoliWiki Page)

PTHR21499:SF6

Superfamily (EcoliWiki Page)

SUPERFAMILY:53633

Superfamily (EcoliWiki Page)

SUPERFAMILY:55021

Superfamily (EcoliWiki Page)

SUPERFAMILY:55021

Superfamily (EcoliWiki Page)

SUPERFAMILY:55347

EcoCyc

EcoCyc:EG10998

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10998

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000987

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0991

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000008

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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